Issue 6, 2008
From the journal:

Molecular BioSystems

Control of bacterial biofilms with marine alkaloid derivatives†‡

Robert W. Huigens III,a   Luyan Ma,b   Christopher Gambino,a   Peter D. R. Moeller,c   Anne Basso,a   John Cavanagh,d   Daniel J. Wozniakb  and  Christian Melander*a  

* Corresponding authors

a Department of Chemistry, North Carolina State University, Raleigh, USA
E-mail: christian_melander@ncsu.edu

b Department of Microbiology and Immunology, Wake Forest University School of Medicine, Winston-Salem, USA

c Center for Coastal Environmental Health and Biomolecular Research, National Oceanic and Atmospheric Administration National Ocean Service, Hollings Marine Laboratory, Charleston, SC, USA

d Department of Molecular and Structural Biochemistry, North Carolina State University, Raleigh, USA

Abstract

Bacterial biofilms are defined as a community of surface-attached bacteria that are protected by an extracellular matrix of biomolecules. We have recently reported the synthesis of a small molecule, denoted TAGE, based on the natural product bromoageliferin and demonstrated that TAGE has anti-biofilm activity against Pseudomonas aeruginosa. Herein we demonstrate that TAGE: (1) does not have selective toxicity against cells within the biofilm state, (2) will inhibit biofilm development under flow conditions, indicating that the CV staining protocol correlates with the ability to be active under biomimetic conditions, and (3) will disperse preformed P. aeruginosa biofilms. We also present preliminary toxicity work that indicates that TAGE is devoid of cytotoxicity in rat and mice cell lines. Advanced derivatives of TAGE have generated compounds shown to be exceedingly effective as biofilm inhibitors against the γ-proteobacteria in this study (P. aeruginosa strains PAO1, PA14, PDO300, and Acinetobacter baumannii). TAGE derivatives also possessed anti-biofilm activity against the β-proteobacterium Bordetella bronchiseptica (Rb50) and the Gram-positive bacterium Staphylococcus aureus;TAGE derivatives inhibited the formation of biofilms, however, some of this activity is attributed to microbicidal activity. The TAGE derivatives presented in this study, however, do not disperse pre-formed biofilms with the same efficiency as TAGE.

Graphical abstract: Control of bacterial biofilms with marine alkaloid derivatives

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Article information

DOI
https://doi.org/10.1039/B719989A
Article type
Paper
Submitted
03 Jan 2008
Accepted
30 Jan 2008
First published
21 Feb 2008

Mol. BioSyst., 2008,4, 614-621

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Control of bacterial biofilms with marine alkaloid derivatives

R. W. Huigens III, L. Ma, C. Gambino, P. D. R. Moeller, A. Basso, J. Cavanagh, D. J. Wozniak and C. Melander, Mol. BioSyst., 2008, 4, 614 DOI: 10.1039/B719989A

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