Issue 7, 2006
From the journal:

Lab on a Chip

Grafting epoxy-modified hydrophilic polymers onto poly(dimethylsiloxane) microfluidic chip to resist nonspecific protein adsorption†

Dapeng Wu,ab   Baoxia Zhao,c   Zhongpeng Dai,a   Jianhua Qina  and  Bingcheng Lin*a  

* Corresponding authors

a 457 Zhongshan Road, Dalian Institute of Chemical Physics, Dalian, China
E-mail: bclin@dicp.ac.cn
Fax: +0086-411-8437-9065
Tel: +0086-411-8437-9065

b Graduate School of the Chinese Academy of Sciences, Dalian, China

c College of Laboratory Medicine, Dalian Medical University, Dalian, China

Abstract

In order to achieve a simple covalent hydrophilic polymer coating on poly(dimethylsiloxane) (PDMS) microfluidic chip, epoxy modified hydrophilic polymers were synthesized in aqueous solution with a persulfate radical initiation system, and crosslinked onto PDMS pretreated by oxygen plasma and silanized with 3-aminopropyl-triethoxysilanes (APTES). Glycidyl methacrylate (GMA) was copolymerized with acrylamide (poly(AAM-co-GMA)) or dimethylacrylamide (poly(DAM-co-GMA)), and graft polymerized with polyvinylpyrrolidone (PVP-g-GMA) or polyvinylalcohol (PVA-g-GMA). The epoxy groups in the polymers were determined by UV spectra after derivation with benzylamine. Reflection absorption infrared spectroscopy (RAIRS) confirmed covalent grafting of GMA-modified polymers onto PDMS surface. Electroosmotic flow (EOF) in the polymer grafted microchannel was strongly suppressed within the range pH 3–11. Surface adsorption of lysozyme and bovine serum albumin (BSA) was reduced to less than 10% relative to that on the native PDMS surface. On the GMA-modified polymer coated PDMS microchip, basic proteins, peptides, and sodium dodecyl sulfate (SDS) denatured proteins were separated successfully.

Graphical abstract: Grafting epoxy-modified hydrophilic polymers onto poly(dimethylsiloxane) microfluidic chip to resist nonspecific protein adsorption

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Article information

DOI
https://doi.org/10.1039/B600765A
Article type
Paper
Submitted
18 Jan 2006
Accepted
21 Apr 2006
First published
05 May 2006

Lab Chip, 2006,6, 942-947

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Grafting epoxy-modified hydrophilic polymers onto poly(dimethylsiloxane) microfluidic chip to resist nonspecific protein adsorption

D. Wu, B. Zhao, Z. Dai, J. Qin and B. Lin, Lab Chip, 2006, 6, 942 DOI: 10.1039/B600765A

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