Issue 3, 2004
From the journal:

Lab on a Chip

Embryonic development in the mouse is enhanced via microchannel culture

Stephanie Raty,a   Eric M. Walters,ab   John Davis,b   Henry Zeringue,c   David J. Beebe,c   Sandra L. Rodriguez-Zasa  and  Matthew B. Wheeler*ab  

* Corresponding authors

a Department of Animal Sciences, University of Illinois, Urbana-Champaign, IL, USA

b Beckman Institute for Advanced Science and Technology, University of Illinois, Urbana-Champaign, IL, USA

c Department of Biomedical Engineering, University of Wisconsin, Madison, WI, USA

Abstract

Microfluidic devices (microchannels) have been fabricated and tested for embryo culture. Three different microfabrication materials (silicon, polydimethylsiloxane (PDMS), and borosilicate) were used to fabricate the microchannels. The objective of this study was to determine if static microchannels permitted culture of mouse embryos to the blastocyst stage. Groups of 10 two-cell ICR × B6SJL/F1 mouse embryos were cultured for 96 hours in 4 different physical culture systems: 1) silicon/borosilicate microchannels, 2) PDMS/borosilicate microchannels, and 3) standard microdrops. Embryos cultured in the silicon/borosilicate and PDMS/borosilicate microchannels exhibited a faster rate of cleavage (P < 0.05), and produced more blastocysts (P < 0.01) than control microdrops. Furthermore, microchannels had a lower percentage of degenerated embryos than control embryos (P < 0.01). The results suggest that the microchannel culture systems may provide a culture environment that more closely mimics the in vivo environment.

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Article information

DOI
https://doi.org/10.1039/B316437C
Article type
Paper
Submitted
16 Dec 2003
Accepted
27 Feb 2004
First published
18 Mar 2004

Lab Chip, 2004,4, 186-190

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Embryonic development in the mouse is enhanced via microchannel culture

S. Raty, E. M. Walters, J. Davis, H. Zeringue, D. J. Beebe, S. L. Rodriguez-Zas and M. B. Wheeler, Lab Chip, 2004, 4, 186 DOI: 10.1039/B316437C

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