Issue 23, 2003
From the journal:

Organic & Biomolecular Chemistry

Heterologous expression in Saccharopolyspora erythraea of a pentaketide synthase derived from the spinosynpolyketide synthase†

Christine J. Martin,*a   Máire C. Timoney,ad   Rose M. Sheridan,a   Steven G. Kendrew,a   Barrie Wilkinson,a   James Stauntonac  and  Peter F. Leadlayab  

* Corresponding authors

a Biotica Technology Ltd, Building 5, Chesterford Research Park, Little Chesterford, Nr Saffron Walden, Essex, UK
E-mail: Christine.Martin@biotica.co.uk

b Cambridge Centre for Molecular Recognition and Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, UK

c Cambridge Centre for Molecular Recognition and Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK

d Pfizer Global Pharmaceuticals, Walton Oaks, Dorking Road, Tadworth, UK

Abstract

A truncated version of the spinosyn polyketide synthase comprising the loading module and the first four extension modules fused to the erythromycin thioesterase domain was expressed in Saccharopolyspora erythraea. A novel pentaketide lactone product was isolated, identifying cryptic steps of spinosyn biosynthesis and indicating the potential of this approach for the biosynthetic engineering of spinosyn analogues. A pathway for the formation of the tetracyclic spinosyn aglycone is proposed.

Graphical abstract: Heterologous expression in Saccharopolyspora erythraea of a pentaketide synthase derived from the spinosyn polyketide synthase

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Article information

DOI
https://doi.org/10.1039/B310740J
Article type
Communication
Submitted
05 Sep 2003
Accepted
14 Oct 2003
First published
29 Oct 2003

Org. Biomol. Chem., 2003,1, 4144-4147

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Heterologous expression in Saccharopolyspora erythraea of a pentaketide synthase derived from the spinosyn polyketide synthase

C. J. Martin, M. C. Timoney, R. M. Sheridan, S. G. Kendrew, B. Wilkinson, J. Staunton and P. F. Leadlay, Org. Biomol. Chem., 2003, 1, 4144 DOI: 10.1039/B310740J

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