Issue 7, 2020

Engineering a chemoenzymatic cascade for sustainable photobiological hydrogen production with green algae

Abstract

Photobiological hydrogen generation is among the most promising routes for the mass production of hydrogen energy. However, the cost and sustainability of photobiological methods largely hamper their large-scale commercial production. Here, we design an anaerobic environment with a constant near-neutral pH for the sustainable induction of green algae flocculation by engineering a robust chemoenzymatic cascade system consisting of glucose, glucose oxidase, catalase, and magnesium hydroxide. We found that Chlamydomonas reinhardtii could stably produce hydrogen in this engineered photobiological system for nearly a month, with an average rate of 0.44 μmol H2 h−1 (mg chlorophyll)−1. This study thus opens a new avenue to photobiological hydrogen production at industrial scales for promising “liquid sunshine” applications.

Graphical abstract: Engineering a chemoenzymatic cascade for sustainable photobiological hydrogen production with green algae

Supplementary files

Article information

Article type
Communication
Submitted
30 Mar 2020
Accepted
12 Jun 2020
First published
12 Jun 2020

Energy Environ. Sci., 2020,13, 2064-2068

Engineering a chemoenzymatic cascade for sustainable photobiological hydrogen production with green algae

J. Chen, J. Li, Q. Li, S. Wang, L. Wang, H. Liu and C. Fan, Energy Environ. Sci., 2020, 13, 2064 DOI: 10.1039/D0EE00993H

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