Issue 77, 2020
From the journal:

Chemical Communications

Intracellular delivery of therapeutic proteins through N-terminal site-specific modification†

Si Si Liew,a   Chengwu Zhang,b   Jie Zhang,c   Hongyan Sun, ORCID logo c   Lin Li ORCID logo *b  and  Shao Q. Yao ORCID logo *a  

* Corresponding authors

a Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore
E-mail: chmyaosq@nus.edu.sg

b Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University, Nanjing 211816, P. R. China
E-mail: iamlli@njtech.edu.cn

c Department of Chemistry and COSDAF (Centre of Super-Diamond and Advanced Films), City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong, China
E-mail: hongysun@cityu.edu.hk

Abstract

A versatile strategy for the intracellular delivery of functional proteins/antibodies was developed using N-terminal site-specific modification. Adopting orthogonal dual-labeling strategies, a cell-permeable RNase A prodrug was designed complementing N-terminal site-specific modification with lysine labeling. Upon successful cytosolic uptake, the prodrug showed reactive oxygen species (ROS)-dependent targeted cancer therapy.

Graphical abstract: Intracellular delivery of therapeutic proteins through N-terminal site-specific modification

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Article information

DOI
https://doi.org/10.1039/D0CC04728G
Article type
Communication
Submitted
08 Jul 2020
Accepted
19 Aug 2020
First published
19 Aug 2020

Chem. Commun., 2020,56, 11473-11476

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Intracellular delivery of therapeutic proteins through N-terminal site-specific modification

S. S. Liew, C. Zhang, J. Zhang, H. Sun, L. Li and S. Q. Yao, Chem. Commun., 2020, 56, 11473 DOI: 10.1039/D0CC04728G

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