Skip to main content
SpringerLink
Log in

Optical manipulation of animal behavior using a ruthenium-based phototrigger

  • PAPER
  • Published:
Photochemical & Photobiological Sciences Aims and scope Submit manuscript

Abstract

A visible-light activatable caged compound based on a ruthenium-polypyridine complex was used to elicit the feeding response of the freshwater cnidarian Hydra vulgaris. The phototrigger delivers L-arginine in a clean reaction under irradiation with blue or green light. The synthesis, characterization and application mode of this caged arginine are described. A combination of fiber-optics setup and a high absor-bance medium allows the precise control of uncaging in the submillimetric range, needed to address the zone where activation takes place.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. S. R. Adams and R. Y. Tsien, Annu. Rev. Physiol., 1993, 55, 755–784.

    Article  CAS  Google Scholar 

  2. G. C. R. Ellis-Davies, Nat. Methods, 2007, 4(8), 619–628.

    Article  CAS  Google Scholar 

  3. C. Brieke, F. Rohrbach, A. Gottschalk, G. Mayer and A. Heckel, Angew. Chem., Int. Ed., 2012, 51, 8446–8476.

    Article  CAS  Google Scholar 

  4. J. Kaplan, B. Forbush III and J. Hoffman, Biochemistry, 1978, 17(10), 1929–1935.

    Article  CAS  Google Scholar 

  5. J. W. Walker, J. A. McCray and G. P. Hess, Biochemistry, 1986, 25, 1799–1805.

    Article  CAS  Google Scholar 

  6. O. Filevich and R. Etchenique, Photochem. Photobiol. Sci., 2013, 12, 1565–1570.

    Article  CAS  Google Scholar 

  7. L. Lameijer, D. Ernst, S. Hopkins, M. Meijer, S. Askes, S. Le Dévédec and S. Bonnet, Angew. Chem., Int. Ed., 2017, 56, 11549–11553.

    Article  CAS  Google Scholar 

  8. A. Bahreman, J. Cuello-Garibo and S. Bonnet, Dalton Trans., 2014, 43, 4494.

    Article  CAS  Google Scholar 

  9. J. Knoll, B. Albani and C. Turro, Acc. Chem. Res., 2015, 48, 2280–2287.

    Article  CAS  Google Scholar 

  10. G. Borth, T. Gallavardin, D. Odgen and P. I. Dalko, Angew. Chem., Int. Ed., 2013, 52, 4526–4537.

    Article  Google Scholar 

  11. R. Araya, V. Andino-Pavlovsky, R. Yuste and R. Etchenique, ACS Chem. Neurosci., 2013, 4(8), 1163–1169.

    Article  CAS  Google Scholar 

  12. K. Glauber, C. E. Dana and R. E. Steele, Curr. Biol., 2010, 20, 964–965.

    Article  Google Scholar 

  13. B. Galliot, Int. J. Dev. Biol., 2012, 56, 411–423.

    Article  CAS  Google Scholar 

  14. C. Dupre and R. Yuste, Curr. Biol., 2017, 27, 1085–1097.

    Article  CAS  Google Scholar 

  15. N. Ji and S. W. Flavell, Curr. Biol., 2017, 27, 297–317.

    Article  Google Scholar 

  16. W. R. Loomis, Annals of New York Academic of Sciences, 1955, 62(9), 211–227.

    Article  Google Scholar 

  17. K. Hanai, J. Comp. Physiol., 1981, 144, 503–508.

    Article  CAS  Google Scholar 

  18. R. Kulkarni and S. Galandes, J. Visualized Exp., 2014, (93), e52178.

    Google Scholar 

  19. L. Zayat, O. Filevich, L. M. Baraldo and R. Etchenique, Philos. Trans. R. Soc., A, 2013, 371, 20120330.

    Article  Google Scholar 

  20. D. Pinnick and B. Durham, Inorg. Chem., 1984, 23, 1440–1445.

    Article  CAS  Google Scholar 

  21. B. Quinn, F. Gagné and C. Blaise, Int. J. Dev. Biol., 2012, 56, 613–625.

    Article  CAS  Google Scholar 

  22. J. A. Carter, C. Hyland, R. E. Steele and E. M. S. Collins, Biophys. J., 2016, 110(5), 1191–1201.

    Article  CAS  Google Scholar 

  23. H. Lenhoff, J. Gen. Physiol., 1961, 45(2), 331–344.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by the National Agency for Science and Technology Promotion, CONICET, and the University of Buenos Aires. R. E. is a member of CONICET.

Author information

Authors and Affiliations

  1. Departamento de Química Inorgánica, Analítica y Química Física, INQUIMAE, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, CONICET, Intendente Güiraldes 2160, Ciudad Universitaria, Pabellón 2, Buenos Aires, C1428EGA, Argentina

    Yeraldith Rojas Pérez & Roberto Etchenique

Authors
  1. Yeraldith Rojas Pérez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Roberto Etchenique
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Roberto Etchenique.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Pérez, Y.R., Etchenique, R. Optical manipulation of animal behavior using a ruthenium-based phototrigger. Photochem Photobiol Sci 18, 208–212 (2019). https://doi.org/10.1039/c8pp00467f

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1039/c8pp00467f

Search

Navigation