Electron transfer rate analysis of a site-specifically wired copper oxidase

Orr Schlesinger; Mor Pasi; Rambabu Dandela; Michael M. Meijler; Lital Alfonta

doi:10.1039/C8CP00041G

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Electron transfer rate analysis of a site-specifically wired copper oxidase†

Orr Schlesinger,

^a Mor Pasi,

^a Rambabu Dandela,‡*^b Michael M. Meijler

*^b and Lital Alfonta

*^a

Author affiliations

* Corresponding authors

^a Department of Life Sciences and Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, PO Box 653, Beer-Sheva, Israel
E-mail: alfontal@bgu.ac.il

^b Department of Chemistry and National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, PO Box 653, Beer-Sheva, Israel
E-mail: meijler@bgu.ac.il

Abstract

Electron transfer kinetic parameters of site-specifically wired copper oxidase were investigated. The enzyme's orientation towards the electrode was controlled by incorporation of propargyl-L-lysine as a site-specific anchoring point. Herein, we demonstrate the importance of immobilization orientation and how it affects electron transfer efficiency and kinetics to each of the enzyme's two active sites.

This article is part of the themed collection: 2018 PCCP HOT Articles

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Article information

DOI: https://doi.org/10.1039/C8CP00041G
Article type: Paper
Submitted: 03 Jan 2018
Accepted: 13 Feb 2018
First published: 13 Feb 2018

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Phys. Chem. Chem. Phys., 2018,20, 6159-6166

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Electron transfer rate analysis of a site-specifically wired copper oxidase

O. Schlesinger, M. Pasi, R. Dandela, M. M. Meijler and L. Alfonta, Phys. Chem. Chem. Phys., 2018, 20, 6159 DOI: 10.1039/C8CP00041G

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Physical Chemistry Chemical Physics

Electron transfer rate analysis of a site-specifically wired copper oxidase†

Abstract

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Electron transfer rate analysis of a site-specifically wired copper oxidase

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