Issue 34, 2017, Issue in Progress
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RSC Advances

Graphene oxide reinforced hydrogels for osteogenic differentiation of human adipose-derived stem cells†

Myungkyung Noh,‡a   Su-Hwan Kim,‡ab   Jiyong Kim,‡a   Ju-Ro Lee,a   Gun-Jae Jeong,a   Jeong-Kee Yoon,a   Seokyung Kang,a   Suk Ho Bhang,c   Hee Hun Yoon,a   Jong-Chan Lee,a   Nathaniel S. Hwang ORCID logo *abd  and  Byung-Soo Kim*abd  

* Corresponding authors

a School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Republic of Korea
E-mail: nshwang@snu.ac.kr, byungskim@snu.ac.kr
Fax: +82-2-880-1604
Tel: +82-2-880-1635

b Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 151-744, Republic of Korea

c School of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Republic of Korea

d N-Bio Institute, Institute of Chemical Processes, Seoul National University, Seoul 151-744, Republic of Korea

Abstract

Polyethylene glycol (PEG)-based hydrogels are attractive biomaterials for stem cell culture due to their tunable material properties and mechanical strength. However, the lack of cell adhesion sites has been one of the major obstacles in generating functional tissue constructs using PEG-based hydrogels. To overcome this limitation, we designed graphene oxide (GO)-functionalized polyethylene glycol diacrylate (PEGDA) hydrogels to assign cell adhesion-dependent biofunctionality. The incorporation of GO into three-dimensional PEGDA networks improved cell attachment, engaged focal adhesion, and activated focal adhesion kinase (FAK) signaling of hydrogel-encapsulated human adipose-derived stem cells (hADSCs). Compared to the control PEGDA hydrogel, GO functionalized PEGDA hydrogel (PEGDA-GO) resulted in enhanced cell viability and survival. When subsequently cultured under osteoinductive condition, PEGDA-GO enhanced osteogenic differentiation and stimulated osteogenic phenotypes compared to those in its PEGDA counterpart. Taken together, GO could serve as an effective biofunctionalizing moiety to modulate stem cell adhesion and differentiation.

Graphical abstract: Graphene oxide reinforced hydrogels for osteogenic differentiation of human adipose-derived stem cells

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Article information

DOI
https://doi.org/10.1039/C7RA02410J
Article type
Paper
Submitted
27 Feb 2017
Accepted
22 Mar 2017
First published
11 Apr 2017
This article is Open Access
Creative Commons BY license

RSC Adv., 2017,7, 20779-20788

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Graphene oxide reinforced hydrogels for osteogenic differentiation of human adipose-derived stem cells

M. Noh, S. Kim, J. Kim, J. Lee, G. Jeong, J. Yoon, S. Kang, S. H. Bhang, H. H. Yoon, J. Lee, N. S. Hwang and B. Kim, RSC Adv., 2017, 7, 20779 DOI: 10.1039/C7RA02410J

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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