Issue 47, 2017
From the journal:

Organic & Biomolecular Chemistry

Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies†

Pietro Dallabernardina,ab   Colin Ruprecht,a   Peter J. Smith,c   Michael G. Hahn, ORCID logo c   Breeanna R. Urbanowicz ORCID logo c  and  Fabian Pfrengle ORCID logo *ab  

* Corresponding authors

a Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476 Potsdam, Germany
E-mail: Fabian.Pfrengle@mpikg.mpg.de

b Freie Universität Berlin, Institute of Chemistry and Biochemistry, Arnimallee 22, 14195 Berlin, Germany

c Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA

Abstract

We report the automated glycan assembly of oligosaccharides related to the plant cell wall hemicellulosic polysaccharide xyloglucan. The synthesis of galactosylated xyloglucan oligosaccharides was enabled by introducing p-methoxybenzyl (PMB) as a temporary protecting group for automated glycan assembly. The generated oligosaccharides were printed as microarrays, and the binding of a collection of xyloglucan-directed monoclonal antibodies (mAbs) to the oligosaccharides was assessed. We also demonstrated that the printed glycans can be further enzymatically modified while appended to the microarray surface by Arabidopsis thaliana xyloglucan xylosyltransferase 2 (AtXXT2).

Graphical abstract: Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies

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Article information

DOI
https://doi.org/10.1039/C7OB02605F
Article type
Communication
Submitted
23 Oct 2017
Accepted
20 Nov 2017
First published
21 Nov 2017
This article is Open Access
Creative Commons BY license

Org. Biomol. Chem., 2017,15, 9996-10000

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Automated glycan assembly of galactosylated xyloglucan oligosaccharides and their recognition by plant cell wall glycan-directed antibodies

P. Dallabernardina, C. Ruprecht, P. J. Smith, M. G. Hahn, B. R. Urbanowicz and F. Pfrengle, Org. Biomol. Chem., 2017, 15, 9996 DOI: 10.1039/C7OB02605F

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