Issue 11, 2017
From the journal:

Chemical Communications

Chemical probing of thiotetronate bio-assembly†

Judith Havemann,a   Marie E. Yurkovich,b   Robert Jenkins,a   Sophia Harringer,a   Weixin Tao,c   Shishi Wen,c   Yuhui Sun,c   Peter F. Leadlayb  and  Manuela Tosin ORCID logo *a  

* Corresponding authors

a Department of Chemistry, University of Warwick, Library Road, UK
E-mail: M.Tosin@warwick.ac.uk
Tel: +44(0) 2476572878

b Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK

c Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, People's Republic of China

Abstract

Chemical ‘chain termination’ probes were utilised for the investigation of thiotetronate antibiotic biosynthesis in the filamentous bacteria Lentzea sp. and Streptomyces thiolactonus NRRL 15439. The use of these tools led to the capture of biosynthetic intermediates involved in the thiotetronate polyketide backbone assembly, providing first insights into substrate specificity and in vivo intermediate processing by unusual iterative synthases.

Graphical abstract: Chemical probing of thiotetronate bio-assembly

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Article information

DOI
https://doi.org/10.1039/C6CC09933E
Article type
Communication
Submitted
14 Dec 2016
Accepted
17 Jan 2017
First published
25 Jan 2017
This article is Open Access
Creative Commons BY license

Chem. Commun., 2017,53, 1912-1915

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Chemical probing of thiotetronate bio-assembly

J. Havemann, M. E. Yurkovich, R. Jenkins, S. Harringer, W. Tao, S. Wen, Y. Sun, P. F. Leadlay and M. Tosin, Chem. Commun., 2017, 53, 1912 DOI: 10.1039/C6CC09933E

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