Issue 81, 2016
From the journal:

Chemical Communications

A robust and quantitative assay platform for multiplexed, high throughput screening of protein kinase inhibitors†

Jieon Lee,a   Il-Soo Park,a   Ginam Park,a   Kyukwang Cho,b   Hee-Sung Parkb  and  Dal-Hee Min*a  

* Corresponding authors

a Center for RNA Research, Institute for Basic Science, Department of Chemistry, Seoul National University, Seoul, Korea
E-mail: dalheemin@snu.ac.kr
Fax: +82-2-889-1568

b Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea

Abstract

We present a new platform for multiplexed protein kinase activity assay using TiO2 decorated graphene oxide (GO), which is applicable to high throughput inhibitor screening. On the basis of the strong affinity of TiO2 for the phosphate group and the fluorescence quenching capability of GO, phosphorylation of substrates by protein kinases was quantitatively measured in a short time.

Graphical abstract: A robust and quantitative assay platform for multiplexed, high throughput screening of protein kinase inhibitors

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Article information

DOI
https://doi.org/10.1039/C6CC05834E
Article type
Communication
Submitted
15 Jul 2016
Accepted
08 Sep 2016
First published
08 Sep 2016

Chem. Commun., 2016,52, 12112-12115

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A robust and quantitative assay platform for multiplexed, high throughput screening of protein kinase inhibitors

J. Lee, I. Park, G. Park, K. Cho, H. Park and D. Min, Chem. Commun., 2016, 52, 12112 DOI: 10.1039/C6CC05834E

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