Issue 4, 2016

Systematic chemoenzymatic synthesis of O-sulfated sialyl Lewis x antigens

Abstract

O-Sulfated sialyl Lewis x antigens play important roles in nature. However, due to their structural complexity, they are not readily accessible by either chemical or enzymatic synthetic processes. Taking advantage of a bacterial sialyltransferase mutant that can catalyze the transfer of different sialic acid forms from the corresponding sugar nucleotide donors to Lewis x antigens, which are fucosylated glycans, as well as an efficient one-pot multienzyme (OPME) sialylation system, O-sulfated sialyl Lewis x antigens containing different sialic acid forms and O-sulfation at different locations were systematically synthesized by chemoenzymatic methods.

Graphical abstract: Systematic chemoenzymatic synthesis of O-sulfated sialyl Lewis x antigens

Supplementary files

Article information

Article type
Edge Article
Submitted
29 Oct 2015
Accepted
14 Dec 2015
First published
17 Dec 2015
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2016,7, 2827-2831

Author version available

Systematic chemoenzymatic synthesis of O-sulfated sialyl Lewis x antigens

A. Santra, H. Yu, N. Tasnima, M. M. Muthana, Y. Li, J. Zeng, N. J. Kenyon, A. Y. Louie and X. Chen, Chem. Sci., 2016, 7, 2827 DOI: 10.1039/C5SC04104J

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements