Bright fluorogenic squaraines with tuned cell entry for selective imaging of plasma membrane vs. endoplasmic reticulum

Mayeul Collot; Rémy Kreder; Anatoliy L. Tatarets; Leonid D. Patsenker; Yves Mely; Andrey S. Klymchenko

doi:10.1039/C5CC06094J

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Bright fluorogenic squaraines with tuned cell entry for selective imaging of plasma membrane vs. endoplasmic reticulum†

Mayeul Collot,*^a Rémy Kreder,^a Anatoliy L. Tatarets,^b Leonid D. Patsenker,^b Yves Mely^a and Andrey S. Klymchenko*^a

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* Corresponding authors

^a Laboratoire de Biophotonique et Pharmacologie, UMR 7213 CNRS, Université de Strasbourg, Faculté de Pharmacie, 74, Route du Rhin, Illkirch, France
E-mail: andrey.klymchenko@unistra.fr, mayeul.collot@unistra.fr

^b The State Scientific Institution “Institute for Single Crystals” of the National Academy of Sciences of Ukraine, 60, Lenin Ave., Kharkiv, Ukraine

Abstract

A rational design of squaraine dyes with lipophilic and zwitterionic groups tunes cell entry, allowing for selective far-red/near-infrared imaging of plasma membrane vs. endoplasmic reticulum. They exhibit up to 110-fold fluorescence enhancement in biomembranes and enable cellular imaging at 1 nM concentration, which make them the brightest membrane probes to date.

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Article information

DOI: https://doi.org/10.1039/C5CC06094J
Article type: Communication
Submitted: 21 Jul 2015
Accepted: 29 Sep 2015
First published: 29 Sep 2015
This article is Open Access

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Chem. Commun., 2015,51, 17136-17139

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Bright fluorogenic squaraines with tuned cell entry for selective imaging of plasma membrane vs. endoplasmic reticulum

M. Collot, R. Kreder, A. L. Tatarets, L. D. Patsenker, Y. Mely and A. S. Klymchenko, Chem. Commun., 2015, 51, 17136 DOI: 10.1039/C5CC06094J

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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Chemical Communications

Bright fluorogenic squaraines with tuned cell entry for selective imaging of plasma membrane vs. endoplasmic reticulum†

Abstract

Supplementary files

Article information

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Bright fluorogenic squaraines with tuned cell entry for selective imaging of plasma membrane vs. endoplasmic reticulum

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