Issue 81, 2014

Chemical biology of genomic DNA: minimizing PCR bias

Abstract

The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde-reactive biotinylation probe.

Graphical abstract: Chemical biology of genomic DNA: minimizing PCR bias

Supplementary files

Article information

Article type
Communication
Submitted
03 Jul 2014
Accepted
15 Aug 2014
First published
18 Aug 2014
This article is Open Access
Creative Commons BY license

Chem. Commun., 2014,50, 12047-12049

Author version available

Chemical biology of genomic DNA: minimizing PCR bias

G. R. McInroy, E. Raiber and S. Balasubramanian, Chem. Commun., 2014, 50, 12047 DOI: 10.1039/C4CC05107F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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