Issue 3, 2013
From the journal:

Molecular BioSystems

Fluorogenic label to quantify the cytosolic delivery of macromolecules†

Tzu-Yuan Chao‡a  and  Ronald T. Raines*ab  

* Corresponding authors

a Department of Biochemistry, University of Wisconsin–Madison, 433 Babcock Drive, Madison, WI, USA
E-mail: rtraines@wisc.edu

b Department of Chemistry, University of Wisconsin–Madison, 1101 University Avenue, Madison, WI, USA

Abstract

The delivery of a macromolecule to the cytosol of human cells is assessed by using a pendant di-O-glycosylated derivative of fluorescein. Its fluorescence is unmasked by Escherichia coli β-galactosidase installed in the cytosol. Background is diminished by using RNAi to suppress the expression of GLB1, which encodes a lysosomal β-galactosidase. This strategy was used to quantify the cytosolic entry of a highly cationic protein, ribonuclease A.

Graphical abstract: Fluorogenic label to quantify the cytosolic delivery of macromolecules

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Article information

DOI
https://doi.org/10.1039/C3MB25552B
Article type
Communication
Submitted
04 Dec 2012
Accepted
10 Jan 2013
First published
10 Jan 2013

Mol. BioSyst., 2013,9, 339-342

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Fluorogenic label to quantify the cytosolic delivery of macromolecules

T. Chao and R. T. Raines, Mol. BioSyst., 2013, 9, 339 DOI: 10.1039/C3MB25552B

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