Issue 99, 2013
From the journal:

Chemical Communications

Sortase A-mediated chemoenzymatic synthesis of complex glycosylphosphatidylinositol-anchored protein†

Zhimeng Wu,a   Xueqing Guo,a   Jian Gaob  and  Zhongwu Guo*ab  

* Corresponding authors

a Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, Michigan 48202, USA
E-mail: zwguo@chem.wayne.edu
Fax: +1-313-577-8822
Tel: +1-313-577-2557

b National Glycoengineering Research Center, Shandong University, Jinan 250100, China

Abstract

Green fluorescent protein and a glycosylphosphatidylinositol (GPI) anchor containing the common core structure and a lipid chain were synthesized and then coupled together in the promotion of bacterial sortase A (SrtA), which was the first example for the synthesis of a full-size GPI-anchored protein by SrtA, demonstrating that this can be a generally useful method for GPI-anchored protein synthesis.

Graphical abstract: Sortase A-mediated chemoenzymatic synthesis of complex glycosylphosphatidylinositol-anchored protein

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Article information

DOI
https://doi.org/10.1039/C3CC47229A
Article type
Communication
Submitted
22 Sep 2013
Accepted
21 Oct 2013
First published
22 Oct 2013

Chem. Commun., 2013,49, 11689-11691

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Sortase A-mediated chemoenzymatic synthesis of complex glycosylphosphatidylinositol-anchored protein

Z. Wu, X. Guo, J. Gao and Z. Guo, Chem. Commun., 2013, 49, 11689 DOI: 10.1039/C3CC47229A

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