Issue 1, 2013
From the journal:

Chemical Science

Expanding DNAzyme functionality through enzyme cascades with applications in single nucleotide repair and tunable DNA-directed assembly of nanomaterials†

Yu Xiang,a   Zidong Wang,b   Hang Xinga  and  Yi Lu*ab  

* Corresponding authors

a Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

b Department of Material Science, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
E-mail: yi-lu@illinois.edu
Fax: +1 217-244-3186
Tel: +1 217-333-2619

Abstract

Many biological functions require two or more enzymes working together in cascades. While many examples of protein and RNA enzyme cascades are known, few enzyme cascades containing solely DNAzymes have been reported. Herein we demonstrate the combination of an 8–17 DNAzyme with RNA cleavage activity and an E47 DNAzyme with DNA ligation activity to achieve a new function of single ribonucleotide repair in DNA while maintaining the integrity of the original DNA sequence, which is difficult for a single DNAzyme to achieve. In addition, this method is applied to modify the sequences of DNA strands immobilized on the surface of nanoparticles to control the DNA-directed assembly selectively and sequentially. Such an approach can be applied to other DNAzymes with different activities to expand the functions of DNAzymes and the scope of their applications.

Graphical abstract: Expanding DNAzyme functionality through enzyme cascades with applications in single nucleotide repair and tunable DNA-directed assembly of nanomaterials

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Article information

DOI
https://doi.org/10.1039/C2SC20763J
Article type
Edge Article
Submitted
15 Jun 2012
Accepted
19 Sep 2012
First published
21 Sep 2012

Chem. Sci., 2013,4, 398-404

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Expanding DNAzyme functionality through enzyme cascades with applications in single nucleotide repair and tunable DNA-directed assembly of nanomaterials

Y. Xiang, Z. Wang, H. Xing and Y. Lu, Chem. Sci., 2013, 4, 398 DOI: 10.1039/C2SC20763J

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