Issue 27, 2012
From the journal:

Organic & Biomolecular Chemistry

Incorporation of a fluorous diazirine group into phosphatidylinositol 4,5-bisphosphate to illustrate its interaction with ADP-ribosylation factor 1†

Weigang Huang,a   Wei Sun,a   Zhiquan Song,a   Yanbao Yu,b   Xian Chenb  and  Qisheng Zhang*a  

* Corresponding authors

a Division of Chemical Biology and Medicinal Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
E-mail: qszhang@unc.edu

b Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA

Abstract

Phosphatidylinositides are one family of the most versatile signaling molecules in cells, yet how they interact with different proteins to regulate biological processes is not well understood. Towards a general strategy to identify phosphatidylinositide–protein interactions, a fluorous diazirine group has been incorporated into phosphatidylinositol 4,5-bisphosphate (PIP2). The modified PIP2 was effectively cleaved by phospholipase C, one signaling protein that utilizes PIP2 as its endogenous substrate. Upon light illumination, the PIP2 probe effectively crosslinks with small GTPase ADP-ribosylation 1 to form a complex, suggesting that the probe might be suitable to identify PIP2-interacting proteins on the proteome level.

Graphical abstract: Incorporation of a fluorous diazirine group into phosphatidylinositol 4,5-bisphosphate to illustrate its interaction with ADP-ribosylation factor 1

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Article information

DOI
https://doi.org/10.1039/C2OB25276G
Article type
Communication
Submitted
06 Feb 2012
Accepted
01 Jun 2012
First published
01 Jun 2012

Org. Biomol. Chem., 2012,10, 5197-5201

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Incorporation of a fluorous diazirine group into phosphatidylinositol 4,5-bisphosphate to illustrate its interaction with ADP-ribosylation factor 1

W. Huang, W. Sun, Z. Song, Y. Yu, X. Chen and Q. Zhang, Org. Biomol. Chem., 2012, 10, 5197 DOI: 10.1039/C2OB25276G

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