Issue 3, 2012
From the journal:

Molecular BioSystems

A C-di-GMP–proflavine–hemin supramolecular complex has peroxidase activity—implication for a simple colorimetric detection†

Shizuka Nakayama,a   Kevin Roelofs,b   Vincent T. Leeb  and  Herman O. Sintim*a  

* Corresponding authors

a Department of Chemistry and Biochemistry, University of Maryland, College Park, USA
E-mail: hsintim@umd.edu
Fax: +1 3013149121
Tel: +1 301-405-0633

b Department of Molecular and Cell Biology, University of Maryland, College Park, USA

Abstract

Herein, we demonstrate that the bacterial signaling molecule, c-di-GMP, can enhance the peroxidation of hemin when proflavine is present. The c-di-GMP–proflavine–hemin nucleotidezyme can oxidize the colorless compound 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), ABTS, to the colored radical cation ABTS˙+ and hence provides simple colorimetric detection of c-di-GMP at low micromolar concentrations.

Graphical abstract: A C-di-GMP–proflavine–hemin supramolecular complex has peroxidase activity—implication for a simple colorimetric detection

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Article information

DOI
https://doi.org/10.1039/C2MB05430B
Article type
Communication
Submitted
18 Oct 2011
Accepted
03 Jan 2012
First published
18 Jan 2012

Mol. BioSyst., 2012,8, 726-729

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A C-di-GMP–proflavine–hemin supramolecular complex has peroxidase activity—implication for a simple colorimetric detection

S. Nakayama, K. Roelofs, V. T. Lee and H. O. Sintim, Mol. BioSyst., 2012, 8, 726 DOI: 10.1039/C2MB05430B

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