Issue 50, 2012
From the journal:

Chemical Communications

Isobaric cross-sequence labeling of peptides by using site-selective N-terminus dimethylation†

Hongqiang Qin,a   Fangjun Wang,a   Yi Zhang,a   Zhengyan Hu,a   Chunxia Song,a   Ren'an Wu,a   Mingliang Yea  and  Hanfa Zou*a  

* Corresponding authors

a CAS Key Laboratory of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, China
E-mail: hanfazou@dicp.ac.cn

Abstract

Highly site-selective dimethyl labeling of N-terminus of peptides has been obtained by adjusting the acidic strength of the reaction solution. And this selective labeling strategy combined with the use of different isotope formaldehyde reagents has been successfully used in the proteome quantification by using the isobaric peptide cross-sequence labeling method, which would play increasingly important roles in the clinical diagnosis, especially in the discovery of biomarkers for diseases.

Graphical abstract: Isobaric cross-sequence labeling of peptides by using site-selective N-terminus dimethylation

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Article information

DOI
https://doi.org/10.1039/C2CC31705B
Article type
Communication
Submitted
07 Mar 2012
Accepted
01 May 2012
First published
01 May 2012

Chem. Commun., 2012,48, 6265-6267

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Isobaric cross-sequence labeling of peptides by using site-selective N-terminus dimethylation

H. Qin, F. Wang, Y. Zhang, Z. Hu, C. Song, R. Wu, M. Ye and H. Zou, Chem. Commun., 2012, 48, 6265 DOI: 10.1039/C2CC31705B

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