Issue 3, 2012
From the journal:

Analyst

Quantitative analysis of urea in human urine and serum by 1H nuclear magnetic resonance†

Lingyan Liu,a   Huaping Mo,b   Siwei Weic  and  Daniel Raftery*c  

* Corresponding authors

a Weldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Dr, West Lafayette, IN

b Purdue Interdepartmental NMR Facility, Purdue University, West Lafayette, IN

c Department of Chemistry, Purdue University, 560 Oval Dr, West Lafayette, IN
E-mail: raftery@purdue.edu
Fax: (+765) 494-0239
Tel: (+765) 494-6070

Abstract

A convenient and fast method for quantifying urea in biofluids is demonstrated using NMR analysis and the solvent water signal as a concentration reference. The urea concentration can be accurately determined with errors less than 3% between 1 mM and 50 mM, and less than 2% above 50 mM in urine and serum. The method is promising for various applications with advantages of simplicity, high accuracy, and fast non-destructive detection. With an ability to measure other metabolites simultaneously, this NMR method is also likely to find applications in metabolic profiling and system biology.

Graphical abstract: Quantitative analysis of urea in human urine and serum by 1H nuclear magnetic resonance

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C2AN15780B
Article type
Paper
Submitted
26 Aug 2011
Accepted
05 Dec 2011
First published
16 Dec 2011

Analyst, 2012,137, 595-600

Permissions

Request permissions

Quantitative analysis of urea in human urine and serum by 1H nuclear magnetic resonance

L. Liu, H. Mo, S. Wei and D. Raftery, Analyst, 2012, 137, 595 DOI: 10.1039/C2AN15780B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements