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Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters

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Abstract

The fluorescence of silver clusters encapsulated by single stranded oligo-DNA (24 cytosine base pairs, C24:Agn) was used to monitor the transfection of this new silver/DNA fluorophore inside living HeLa cells. For this, the C24:Agn molecules were complexed with a commercially available transfection reagent Lipofectamine and the internalization of C24:Agn was followed with confocal fluorescence microscopy. Bright near-infrared fluorescence was observed from inside the transfected HeLa cells, when exciting with 633 nm excitation, opening up the possibility for the use of these C24:Agn clusters for biological labelling and imaging of living cells and for monitoring the transfection process with limited harm to the living cells.

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Authors and Affiliations

  1. Department of Chemistry, Katholieke Universiteit Leuven, Celestijnenlaan 200F, 3001, Heverlee, Belgium

    Yasuko Antoku, Jun-ichi Hotta, Hideaki Mizuno, Johan Hofkens & Tom Vosch

  2. School of Chemistry and Biochemistry and Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 901 Atlantic Drive, Atlanta, GA, 30332-0400, USA

    Robert M. Dickson

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  1. Yasuko Antoku
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  2. Jun-ichi Hotta
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  3. Hideaki Mizuno
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  4. Robert M. Dickson
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  5. Johan Hofkens
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Correspondence to Johan Hofkens or Tom Vosch.

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Antoku, Y., Hotta, Ji., Mizuno, H. et al. Transfection of living HeLa cells with fluorescent poly-cytosine encapsulated Ag nanoclusters. Photochem Photobiol Sci 9, 716–721 (2010). https://doi.org/10.1039/c0pp00015a

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