Issue 12, 2010
From the journal:

Organic & Biomolecular Chemistry

Visualization of G-quadruplexes by using a BODIPY-labeled macrocyclic heptaoxazole†

Masayuki Tera,a   Keisuke Iida,a   Kazunori Ikebukuro,a   Hiroyuki Seimiya,b   Kazuo Shin-yac  and  Kazuo Nagasawa*a  

* Corresponding authors

a Department of Biotechnology and Life Science Faculty of Technology, Tokyo University of Agriculture and Technology (TUAT), Koganei, Tokyo 184-8588, Japan
E-mail: knaga@cc.tuat.ac.jp
Fax: +81-42-388-7295
Tel: +81-42-388-7295

b Cancer Chemotherapy Center, Japanese Foundation for Cancer Research, Koto-ku, Tokyo 135-8550, Japan

c Biological Information Research Center National Institute of Advanced Industrial Science and Technology, Koto-ku, Tokyo 135-0064, Japan

Abstract

A BODIPY-labeled macrocyclic heptaoxazole, L1BOD-7OTD, was developed as a fluorescent ligand for G-quadruplexes. The results of the study show that L1BOD-7OTD both selectively induces the formation of intramolecular G-quadruplexes from some G-quadruplex forming oligonucleotides (GFOs). In addition, the labelled macrocyclic heptaozaxole strongly binds to and stabilizes intramolecular G-quadruplexes. Moreover, this substance can be used to directly visualize the G-quadruplexes in the form of green fluorescence. Finally, the possibility that G-quadruplexes form in the cells was demonstrated by using of L1BOD-7OTD.

Graphical abstract: Visualization of G-quadruplexes by using a BODIPY-labeled macrocyclic heptaoxazole

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Article information

DOI
https://doi.org/10.1039/C002117B
Article type
Paper
Submitted
02 Feb 2010
Accepted
30 Mar 2010
First published
22 Apr 2010

Org. Biomol. Chem., 2010,8, 2749-2755

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Visualization of G-quadruplexes by using a BODIPY-labeled macrocyclic heptaoxazole

M. Tera, K. Iida, K. Ikebukuro, H. Seimiya, K. Shin-ya and K. Nagasawa, Org. Biomol. Chem., 2010, 8, 2749 DOI: 10.1039/C002117B

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