Issue 12, 2009
From the journal:

Lab on a Chip

Selective and tunable gradient device for cell culture and chemotaxis study†

Dongshin Kim,‡ab   Mary A. Lokuta,c   Anna Huttenlochercd  and  David J. Beebe*ab  

* Corresponding authors

a Department of Mechanical Engineering, University of Wisconsin, Madison, WI, USA

b Department of Biomedical Engineering, University of Wisconsin, 2142 Engineering Centers Building, 1550 Engineering Drive, Madison, WI, USA
E-mail: djbeebe@wisc.edu
Tel: (+608) 262-2260

c Department of Pediatrics, 4205 Microbial Sciences Building, 1550 Linden Dr, Madison, WI, USA

d Department of Pharmacology, University of Wisconsin, Madison, WI

Abstract

This article describes a microfluidic device for cell culture and chemotaxis studies under various temporal and spatial concentration gradients of the medium or chemoattractant. Vertical membranes formed using in situ fabrication are employed to avoid fluid flow inside the cell observation chamber. Thus, the medium and chemoattractants are primarily provided by diffusion, maintaining cell–cell communicationvia secreted factors. Neutrophils were used to demonstrate the capability of the device for chemotaxis research. Experiments exhibited successful migration up a concentration gradient of interleukin 8.

Graphical abstract: Selective and tunable gradient device for cell culture and chemotaxis study

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Article information

DOI
https://doi.org/10.1039/B901613A
Article type
Technical Note
Submitted
27 Jan 2009
Accepted
16 Mar 2009
First published
26 Mar 2009

Lab Chip, 2009,9, 1797-1800

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Selective and tunable gradient device for cell culture and chemotaxis study

D. Kim, M. A. Lokuta, A. Huttenlocher and D. J. Beebe, Lab Chip, 2009, 9, 1797 DOI: 10.1039/B901613A

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