Issue 22, 2008
From the journal:

Organic & Biomolecular Chemistry

Selectivity and affinity of DNA triplex forming oligonucleotides containing the nucleoside analogues2′-O-methyl-5-(3-amino-1-propynyl)uridine and 2′-O-methyl-5-propynyluridine†

Hong Li,a   Paul S. Millerb  and  Michael M. Seidman*a  

* Corresponding authors

a Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, Baltimore, Maryland 21224, USA
E-mail: seidmanm@grc.nia.nih.gov
Fax: +1-410-558-8157
Tel: +1-410-558-8565

b Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA
E-mail: pmiller@jhsph.edu
Fax: +1-410-955-2926
Tel: +1-410-955-3489

Abstract

Triplex forming oligonucleotides (TFOs) containing the nucleoside analogues 2′-O-methyl-5-propynyluridine (1) and 2′-O-methyl-5-(3-amino-1-propynyl)uridine (2) were synthesized. The affinity and selectivity of triplex formation by these TFOs were studied by gel shift analysis, Tm value measurement, and association rate assays. The results show that the introduction of 1 and 2 into TFOs can improve the stability of the triplexes under physiological conditions. Optimized distribution of 1 or 2 in the TFOs combined with a cluster of contiguous nucleosides with 2′-aminoethoxy sugars resulted in formation of triplexes with further enhanced stability and improved selectivity.

Graphical abstract: Selectivity and affinity of DNA triplex forming oligonucleotides containing the nucleoside analogues 2′-O-methyl-5-(3-amino-1-propynyl)uridine and 2′-O-methyl-5-propynyluridine

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/B810709B
Article type
Paper
Submitted
24 Jun 2008
Accepted
13 Aug 2008
First published
23 Sep 2008

Org. Biomol. Chem., 2008,6, 4212-4217

Permissions

Request permissions

Selectivity and affinity of DNA triplex forming oligonucleotides containing the nucleoside analogues 2′-O-methyl-5-(3-amino-1-propynyl)uridine and 2′-O-methyl-5-propynyluridine

H. Li, P. S. Miller and M. M. Seidman, Org. Biomol. Chem., 2008, 6, 4212 DOI: 10.1039/B810709B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements