Issue 7, 2008
From the journal:

Lab on a Chip

An integrated optofluidic platform for Raman-activated cell sorting†

Adrian Y. Lau,ab   Luke P. Leeb  and  James W. Chan*ac  

* Corresponding authors

a Applied Physics and Biophysics Division, Lawrence Livermore National Laboratory, P.O. Box 808, L-211, Livermore, CA, USA
E-mail: chan19@llnl.gov

b Department of Bioengineering, University of California–Berkeley, Berkeley, CA, USA

c NSF Center for Biophotonics Science and Technology, University of California–Davis, Sacramento, CA, USA

Abstract

We report on integrated optofluidic Raman-activated cell sorting (RACS) platforms that combine multichannel microfluidic devices and laser tweezers Raman spectroscopy (LTRS) for delivery, identification, and simultaneous sorting of individual cells. The system allows label-free cell identification based on Raman spectroscopy and automated continuous cell sorting. Two optofluidic designs using hydrodynamic focusing and pinch-flow fractionation are evaluated based on their sorting design and flow velocity effect on the laser trapping efficiency at different laser power levels. A proof-of-principle demonstration of the integrated optofluidic LTRS system for the identification and sorting of two leukemia cell lines is presented. This functional prototype lays the foundation for the development of a label-free cell sorting platform based on intrinsic Raman markers for automated sampling and sorting of a large number of individual cells in solution.

Graphical abstract: An integrated optofluidic platform for Raman-activated cell sorting

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Article information

DOI
https://doi.org/10.1039/B803598A
Article type
Paper
Submitted
06 Mar 2008
Accepted
24 Apr 2008
First published
28 May 2008

Lab Chip, 2008,8, 1116-1120

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An integrated optofluidic platform for Raman-activated cell sorting

A. Y. Lau, L. P. Lee and J. W. Chan, Lab Chip, 2008, 8, 1116 DOI: 10.1039/B803598A

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