Issue 22, 2002
From the journal:

Journal of the Chemical Society, Perkin Transactions 1

Polysaccharide–polynucleotide complexes. Part 12.† Enhanced affinity for various polynucleotide chains by site-specific chemical modification of schizophyllan‡

Kazuya Koumoto,a   Taro Kimura,b   Masami Mizu,a   Toyoki Kunitake,a   Kazuo Sakurai*a  and  Seiji Shinkai*c  

* Corresponding authors

a Department of Chemical Processes and Environments, Faculty of Environmental Engineering, The University of Kitakyushu, 1-1 Hibikino, Wakamatsu-ku, Fukuoka 808-0135, Japan

b Fukuoka Industrial Technology Center, Biotechnology and Food Research Institute, 1465-5 Aikawa Kurume, Fukuoka 839-0861, Japan

c Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Fukuoka 812-8581, Japan

Abstract

Recently, we found that schizophyllan (SPG), which is a natural and neutral polysaccharide, can form macromolecular complexes with certain polynucleotides. In order to increase the stability and the affinity of the complexes, we synthesized a 2-aminoethanol-appended SPG (N-s-SPG) by utilizing periodate oxidation and reductive amination. Although the modification level was only 2.4 ± 0.3%, the melting temperature of the poly(C)–N-s-SPG complex was increased by 8 °C comparing with the poly(C)–s-SPG complex. In addition, the conformation of the complex was scarcely perturbed by the introduction of amino groups. When we formed complexes of N-s-SPG and other polynucleotides, the melting temperature of their complexes increased without exception. Moreover, the amino modification induced complexation with poly(U), which does not form a complex with SPG. The affinity for the short chain nucleotide was also enhanced. These results indicate that the present modification method is quite useful to improve complex stability and we believe, therefore, that this strategy will make it possible to apply SPG to a novel gene carrier.

Graphical abstract: Polysaccharide–polynucleotide complexes. Part 12. Enhanced affinity for various polynucleotide chains by site-specific chemical modification of schizophyllan

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Article information

DOI
https://doi.org/10.1039/B207553A
Article type
Paper
Submitted
01 Aug 2002
Accepted
09 Sep 2002
First published
24 Oct 2002

J. Chem. Soc., Perkin Trans. 1, 2002, 2477-2484

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Polysaccharide–polynucleotide complexes. Part 12. Enhanced affinity for various polynucleotide chains by site-specific chemical modification of schizophyllan

K. Koumoto, T. Kimura, M. Mizu, T. Kunitake, K. Sakurai and S. Shinkai, J. Chem. Soc., Perkin Trans. 1, 2002, 2477 DOI: 10.1039/B207553A

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