An integrated microfluidic biochemical detection system for protein analysis with magnetic bead-based sampling capabilities

Jin-Woo Choi; Kwang W. Oh; Jennifer H. Thomas; William R. Heineman; H. Brian Halsall; Joseph H. Nevin; Arthur J. Helmicki; H. Thurman Henderson; Chong H. Ahn

doi:10.1039/B107540N

An integrated microfluidic biochemical detection system for protein analysis with magnetic bead-based sampling capabilities

Jin-Woo Choi,*^a Kwang W. Oh,^a Jennifer H. Thomas,^b William R. Heineman,^b H. Brian Halsall,^b Joseph H. Nevin,^a Arthur J. Helmicki,^a H. Thurman Henderson^a and Chong H. Ahn^a

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* Corresponding authors

^a Department of Electrical and Computer Engineering and Computer Science, University of Cincinnati, Cincinnati, OH 45221-0030, USA
E-mail: choijw@email.uc.edu
Fax: +1-513-556-7326
Tel: +1-513-556-2229

^b Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221-0030, USA

Abstract

This paper presents the development and characterization of an integrated microfluidic biochemical detection system for fast and low-volume immunoassays using magnetic beads, which are used as both immobilization surfaces and bio-molecule carriers. Microfluidic components have been developed and integrated to construct a microfluidic biochemical detection system. Magnetic bead-based immunoassay, as a typical example of biochemical detection and analysis, has been successfully performed on the integrated microfluidic biochemical analysis system that includes a surface-mounted biofilter and electrochemical sensor on a glass microfluidic motherboard. Total time required for an immunoassay was less than 20 min including sample incubation time, and sample volume wasted was less than 50 μl during five repeated assays. Fast and low-volume biochemical analysis has been successfully achieved with the developed biofilter and immunosensor, which is integrated to the microfluidic system. Such a magnetic bead-based biochemical detection system, described in this paper, can be applied to protein analysis systems.

Article information

https://doi.org/10.1039/B107540N

Article type

Paper

Submitted

20 Aug 2001

Accepted

02 Nov 2001

First published

06 Dec 2001

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Lab Chip, 2002,2, 27-30

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An integrated microfluidic biochemical detection system for protein analysis with magnetic bead-based sampling capabilities

J. Choi, K. W. Oh, J. H. Thomas, W. R. Heineman, H. B. Halsall, J. H. Nevin, A. J. Helmicki, H. T. Henderson and C. H. Ahn, Lab Chip, 2002, 2, 27 DOI: 10.1039/B107540N

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An integrated microfluidic biochemical detection system for protein analysis with magnetic bead-based sampling capabilities

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