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Expression and function of human steroid receptor RNA activator in prostate cancer cells: role of endogenous hSRA protein in androgen receptor-mediated transcription

Prostate Cancer and Prostatic Diseases volume 9, pages 173–178 (2006)Cite this article

Abstract

Steroid receptor RNA activator (SRA) was first isolated as a steroid receptor co-activator that functioned as an RNA transcript. Later, we demonstrated that SRA needs to be translated in order to co-activate androgen receptor (AR). Here, we showed that three isoforms of human SRA enhanced AR activities. Small interfering RNA against SRA suppressed AR activities in PC-3 cells transfected with pSG5AR and in LNCaP cells that have an endogenous mutated-AR. Western blot showed that SRA protein was expressed at a higher level in PC-3 than in LNCaP cells, suggesting that SRA may be related to hormone-independent growth of prostate cancer.

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Abbreviations

SRA:

steroid receptor RNA activator

hSRA:

human steroid receptor RNA activator

AR:

androgen receptor

siRNA:

small interfering RNA

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Acknowledgements

We thank Dr Chawnshang Chang for providing pSG5AR. We also thank NV Organon and Nippon Kayaku Corp. for the materials. We thank Keiko Onishi and Sakae Masaki for technical assistance. This work was supported by grants from the Ministry of Education, Science, and Culture of Japan, and Osaka City University Medical Research Foundation.

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Authors and Affiliations

  1. Department of Urology, Osaka City University Graduate School of Medicine, Osaka, Japan

    T Kurisu, T Tanaka, J Ishii, K Matsumura, K Sugimura, T Nakatani & H Kawashima

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  1. T Kurisu
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  2. T Tanaka
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  3. J Ishii
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Correspondence to H Kawashima.

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Kurisu, T., Tanaka, T., Ishii, J. et al. Expression and function of human steroid receptor RNA activator in prostate cancer cells: role of endogenous hSRA protein in androgen receptor-mediated transcription. Prostate Cancer Prostatic Dis 9, 173–178 (2006). https://doi.org/10.1038/sj.pcan.4500867

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