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Differential immunization identifies PHB1/PHB2 as blood-borne tumor antigens

Oncogene volume 23, pages 7430–7435 (2004)Cite this article

Abstract

Early diagnosis of cancer is crucial for successful treatment. Noninvasive assays for detecting tumor-derived antigens in serum and other bodily fluids have the potential to screen healthy individuals for hitherto undetected cancers. Very few such assays have been successfully developed, in part because identifying potential target antigens remains a challenge. To identify new blood-borne tumor antigens for the purpose of establishing such assays, we have developed a novel technique called differential immunization. Using this method, we have identified PHB1 and PHB2, proteins thought to function as mitochondrial chaperones and transcriptional regulators, as antigens released from colorectal tumors in vivo. Serum from colorectal patients contains significantly higher levels of these antigens compared to serum from healthy volunteers. These data demonstrate that differential immunization is an effective new method for identifying tumor-derived antigens in serum.

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Acknowledgements

We are very grateful to Norma Howells and Selma Huber for excellent assistance with animal experiments, and to Phil Coates for helpful discussions. This work was supported by a grant to JPS by the Association for International Cancer Research (AICR).

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Authors and Affiliations

  1. Forschungszentrum Karlsruhe, Institut für Toxikologie und Genetik, Postfach 3640, Karlsruhe, D-76021, Germany

    Jörg Mengwasser, Angela Piau & Jonathan P Sleeman

  2. Robert-Rössle-Clinic, Humboldt-University, Charité, Lindenberger Weg 80, Berlin, D-13125, Germany

    Peter Schlag

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  1. Jörg Mengwasser
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Correspondence to Jonathan P Sleeman.

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Mengwasser, J., Piau, A., Schlag, P. et al. Differential immunization identifies PHB1/PHB2 as blood-borne tumor antigens. Oncogene 23, 7430–7435 (2004). https://doi.org/10.1038/sj.onc.1207987

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