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  • Oncogenomics
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Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma

Oncogene volume 22, pages 298–307 (2003)Cite this article

Abstract

An analytic strategy was followed to identify putative regulatory genes during the development of human hepatocellular carcinoma (HCC). This strategy employed a bioinformatics analysis that used a database search to identify genes, which are differentially expressed in human HCC and are also under cell cycle regulation. A novel cell cycle regulated gene (HURP) that is overexpressed in HCC was identified. Full-length cDNAs encoding the human and mouse HURP genes were isolated. They share 72 and 61% identity at the nucleotide level and amino-acid level, respectively. Endogenous levels of HURP mRNA were found to be tightly regulated during cell cycle progression as illustrated by its elevated expression in the G2/M phase of synchronized HeLa cells and in regenerating mouse liver after partial hepatectomy. Immunofluorescence studies revealed that hepatoma up-regulated protein (HURP) localizes to the spindle poles during mitosis. Overexpression of HURP in 293T cells resulted in an enhanced cell growth at low serum levels and at polyhema-based, anchorage-independent growth assay. Taken together, these results strongly suggest that HURP is a potential novel cell cycle regulator that may play a role in the carcinogenesis of human cancer cells.

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Acknowledgements

We thank Mien-Chie Hung (The University of Texas MD Anderson Cancer Center) for critical reading of the manuscript and Kuo-Ji Chen, Yu-Lun Liao, Min-Pey Ding, and Jung-Mao Hsu for their excellent technical assistance. We also thank Dr Anna F-Y Li for her assistance in patho-logical reading. This work was supported in part by grants from ADImmune Corporation, Program for Promoting Academic Excellence of Universities (89-B-FA22-2-4) and National Health Research Institutes (NHRI-EX90-9001-BL) to APT, MJF, and CKC, grant from the National Health Research Institutes to CFH, and grant from National Science Counsel (NSC-91-2323-B075-001) and Molecular Pathology core laboratory (National Yang Ming University) to CWC.

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Author notes

  1. Ann-Ping Tsou, Chu-Wen Yang and Chi-Ying F Huang: These authors contributed equally to this work.

Authors and Affiliations

  1. Institute of Biotechnology in Medicine, National Yang-Ming University, Taipei, 112, ROC, Taiwan

    Ann-Ping Tsou

  2. Institute of Biochemistry, National Yang-Ming University, Taipei, 112, ROC, Taiwan

    Chu-Wen Yang

  3. Division of Molecular and Genomic Medicine, National Health Research Institutes, Nankang, Taipei, 115, ROC, Taiwan

    Chi-Ying F Huang, Ricky Chang-Tze Yu, Yuan-Chii G Lee & Chen-Kung Chou

  4. Institute of Microbiology and Immunology, National Yang-Ming University, Taipei, 112, ROC, Taiwan

    Cha-Wei Chang

  5. Institute of Genetics, National Yang-Ming University, Taipei, 112, ROC, Taiwan

    Bo-Rue Chen & Yu-Fang Chung

  6. Institute of Neuroscience, National Yang-Ming University, Taipei, 112, ROC, Taiwan

    Ming-Ji Fann

  7. Department of Medical Research and Education, Veterans General Hospital-Taipei 112, ROC, Taiwan

    Chin-Wen Chi & Chen-Kung Chou

  8. Institute of Pharamacology, National Yang-Ming University, Taipei, 112, ROC, Taiwan

    Chin-Wen Chi

  9. Institute of Traditional Medicine, National Yang-Ming University, Taipei, 112, ROC, Taiwan

    Jen-Hwey Chiu

  10. Department of Life Science, National Yang-Ming University, Taipei, 112, ROC, Taiwan

    Chen-Kung Chou

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  1. Ann-Ping Tsou
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Correspondence to Chen-Kung Chou.

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Tsou, AP., Yang, CW., Huang, CY. et al. Identification of a novel cell cycle regulated gene, HURP, overexpressed in human hepatocellular carcinoma. Oncogene 22, 298–307 (2003). https://doi.org/10.1038/sj.onc.1206129

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