Abstract
The cell division cycle is regulated through both transcriptional and post-transcriptional mechanisms. The altered expression of a number of genes at the mRNA level is known to be essential for progression through the cell cycle, however, a comprehensive gene expression profile of human cells remains to be completed. Here we sought to monitor the differential gene expression of genes after the transition of G2 cells into G1 prior to the restriction point. GeneChip containing microarrays of oligonucleotides corresponding to over 12 000 human genes were employed to profile differential gene expression in G1 and G2. After three independent experiments the resultant data was filtered and a set of genes was compiled based on at least threefold-altered expression, no background noise in determining expression and observation in all experiments. Our analysis identified 154 genes that were elevated in G2 phase of cells as compared to early G1 phase including 15 novel genes. This number included mRNAs whose upregulation is known to occur in G2 phase. Surprisingly only 19 genes were upregulated in G1 phase, among these six genes were novel. Some of these genes are candidates for transition through early G1. This gene inventory for G1 and G2 phases of cell cycle will provide the basis for understanding of cell cycle delay as a result of DNA damage.
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References
Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD . 1994 Molecular Biology of the Cell Robertson, M (ed) New York: Garland p 369
Cho RJ, Campbell MJ, Winzeler EA, Steinmetz L, Conway A, Wodicka L, Wolfsberg TG, Gabrielian AE, Landsman D, Lockhart DJ, Davis RW . 1998 Mol. Cell 2: 65–73
Cho RJ, Huang M, Campbell MJ, Dong H, Steinmetz L, Sapinoso L, Hampton G, Elledge SJ, Davis RW, Lockhart DJ . 2001 Nat. Genet. 27: 48–54
Claverie J-M . 2001 Science 291: 1255–1257
DeRisi JL, Iyer VR, Brown PO . 1997 Science 278: 680–686
Eisen MB, Spellman PT, Brown PO, Botstein D . 1998 Proc. Natl. Acad. Sci. USA 95: 14863–14868 http://rana.stanford.edu/software
Ghosh S, Paweletz N, Schroeter D . 1998 Exp. Cell Res. 242: 1–9
Heintz N . 1993 Trends Biochem. Sci. 18: 15–17
Hitomi M, Stacey DW . 2001 FEBS Lett. 490: 123–131
Kato J . 1999 Front. Biosci. 1: 787–79
Kel AE, Kel-Margoulis OV, Farnham PJ, Bartley SM, Wingender E, Zhang MQ . 2001 J. Mol. Biol. 309: 99–120
Kong M, Barnes EA, Ollendorff V, Donoghue DJ . 2000 EMBO J. 19: 1378–1388
Lee SE, Mitchell RA, Cheng A, Hendrickson EA . 1997 Mol. Cell. Biol. 17: 1425–1433
Maity A, McKenna WG, Muschel RJ . 1994 Radiother. Oncol. 31: 1–13
Maity A, McKenna WG, Muschel RJ . 1995 EMBO J. 14: 603–609
Matsushime H, Quelle DE, Shurtleff SA, Shibuya M, Sherr CJ, Kato JY . 1994 Mol. Cell. Biol. 14: 2066–2076
Morgan DO . 1995 Nature 374: 131–134
Murray AW, Marks D . 2001 Nature 409: 844–846
Nakanishi M, Ando H, Watanabe N, Kitamura K, Ito K, Okayama H, Miyamoto T, Agui T, Sasaki M . 2000 Genes Cells 5: 839–847
Pines J . 1991 Cell Growth Differ. 2: 305–310
Sakai N, Ohtsu M, Fujita H, Koike T, Kuzumaki N . 1999 Exp. Cell. Res. 251: 224–233
Schena M, Dhalon D, Davis RW, Brown PO . 1995 Science 270: 467–470
Schena M, Shalon D, Heller R, Chai A, Brown PO, Davis RW . 1996 Proc. Natl. Acad. Sci. USA 93: 10614–10619
Spellman PT, Sherlock G, Zhang MQ, Iyer VR, Anders K, Eisen MB, Brown PO, Botstein D, Futcher B . 1998 Mol. Biol. Cell. 9: 3273–3297
Toyoshima-Morimoto F, Taniguchi E, Shinya N, Iwamatsu A, Nishida E . 2001 Nature 410: 215–220
Vidal A, Koff A . 2000 Gene 247: 1–15
Vindelov LL, Christensen IJ . 1990 Cytometry 11: 753–770
Wang W, Caldwell MC, Lin S, Furneaux H, Gorospe M . 2000 EMBO J. 19: 2340–2350
Werner T . 2001 Pharmacogenomics 2: 25–36
Yang J, Kornbluth S . 1999 Trends Cell. Biol. 9: 207–210
Acknowledgements
This work was supported by NIH grant PO1 CA75138 and RO1 GM47439. The authors wish to thank Jennifer Hartman, Elizabeth Keiper and Vincent Bakanauskas for technical assistance.
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Chaudhry, M., Chodosh, L., McKenna, W. et al. Gene expression profiling of HeLa cells in G1 or G2 phases. Oncogene 21, 1934–1942 (2002). https://doi.org/10.1038/sj.onc.1205264
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DOI: https://doi.org/10.1038/sj.onc.1205264
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