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Thymic hyperplasia and lung carcinomas in a line of mice transgenic for keratin 5-driven HPV16 E6/E7 oncogenes

Oncogene volume 20, pages 8148–8153 (2001)Cite this article

Abstract

Human Papillomavirus type 16 (HPV-16) is the cause of both benign lesions and ano-genital cancers. In HPV-associated cancers the transforming properties of the expressed viral E6 and E7 proteins have been revealed by a number of different assays. We have generated transgenic mice expressing HPV-16 E6/E7 genes under the control of the murine keratin 5 gene promoter, which should confer cell-type specific expression in the basal cells of squamous stratified epithelia. Transgenic mice developed thymic hyperplasia and lung neoplasia with 100% frequency, the thymus showing a size increase at 2 months and reaching the maximum dimension at 6 months, when lung carcinomas appeared. After this time the size of hyperplastic thymi decreased, while malignant formations invaded the mediastinal area. Hepatic metastasis could be also observed in some of the animals at the autopsy and death invariably occurred around 10–11 months of age.

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Acknowledgements

We thank Dr GP Dotto for providing the K5Ti plasmid, Drs Cesira Galeotti and Barry Howes for careful reading of the manuscript. Alessio Benanchi for technical assistance and Giorgio Corsi for artwork.

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Author notes

  1. Lubbertus CF Mulder

    Present address: Aaron Diamond AIDS Research Center, New York, USA

  2. Sabrina Bertini

    Present address: Divisione Bioline, Amplimedical, Buttigliera Alta, 10090, Torino, Italy

Authors and Affiliations

  1. Department of Physiopathology and Experimental Medicine, University of Siena, 53100, Siena, Via Aldo Moro, Italy

    Laura Carraresi & Mara Rossini

  2. Institute of Pathological Anatomy and Histology, University of Siena, Via Le Scotte 4, 53100, Siena, Italy

    Sergio A Tripodi, Karin Schuerfeld & Marcella Cintorino

  3. Chiron Research Center, Via Fiorentina 1, 53100, Siena, Italy

    Lubbertus CF Mulder, Sabrina Bertini, Sandra Nuti, Giuliano Bensi & Marirosa Mora

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  1. Laura Carraresi
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Correspondence to Mara Rossini.

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Carraresi, L., Tripodi, S., Mulder, L. et al. Thymic hyperplasia and lung carcinomas in a line of mice transgenic for keratin 5-driven HPV16 E6/E7 oncogenes. Oncogene 20, 8148–8153 (2001). https://doi.org/10.1038/sj.onc.1205007

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