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Increased susceptibility to carcinogen-induced mammary tumors in MMTV-Cdc25B transgenic mice

Oncogene volume 18, pages 5159–5166 (1999)Cite this article

Abstract

Cdc25 phosphatases activate cyclin-dependent kinases (Cdks) by dephosphorylating critical phospho-tyrosine and phospho-threonine residues on these proteins. Several types of studies indicate that Cdc25s can enhance cell proliferation and oncogenesis. Furthermore, overexpression of Cdc25A and/or B have been detected in several types of primary human cancers, including breast cancers. To further assess the oncogenic capacity of Cdc25B in vivo, we have generated transgenic mice that overexpress Cdc25B in the mammary epithelium, driven by the MMTV – LTR promoter. Although these mice are grossly normal for up to 18 months, the ectopic expression of Cdc25B in their mammary glands increases the susceptibility of these mice to induction of mammary tumors by the carcinogen 9,10-dimethyl-1,2-benzanthracene (DMBA).

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Acknowledgements

We are grateful for the excellent administrative assistance provided by Barbara Castro. We thank Dr David Beach for the Cdc25B2 cDNA and Dr Giorgio Cattoretti for technical assistance and helpful discussions. This work was supported by National Institutes of Health Grant RO1 CA/ES 63467, and an award from the National Foundation for Cancer Research (to Dr I Bernard Weinstein). ED Slosberg was supported by NCI Cancer Training Grant T32CA09503 and a NIH Pre-Doctoral Training Grant T32GM07088.

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Author notes

  1. Yao Yao and Eric D Slosberg: Yao Yao and Eric D Slosberg contributed equally to the present work

Authors and Affiliations

  1. Herbert Irving Comprehensive Cancer Center, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, NY 10032, USA

    Yao Yao, Eric D Slosberg, Wang-Qiu Xing, Regina M Santella & I Bernard Weinstein

  2. Integrated Program in CMBS, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, NY 10032, USA

    Yao Yao & Lei Wang

  3. Department of Genetics, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, NY 10032, USA

    Eric D Slosberg & I Bernard Weinstein

  4. Department of Pathology, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, NY 10032, USA

    Hanina Hibshoosh

  5. Division of Environmental Health Sciences, School of Public Health, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, NY 10032, USA

    Yu-Jing Zhang, Regina M Santella & I Bernard Weinstein

  6. Department of Medicine, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, NY 10032, USA

    I Bernard Weinstein

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  1. Yao Yao
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Yao, Y., Slosberg, E., Wang, L. et al. Increased susceptibility to carcinogen-induced mammary tumors in MMTV-Cdc25B transgenic mice. Oncogene 18, 5159–5166 (1999). https://doi.org/10.1038/sj.onc.1202908

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  • DOI: https://doi.org/10.1038/sj.onc.1202908

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