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Expression of the focal adhesion protein paxillin in lung cancer and its relation to cell motility

Oncogene volume 18, pages 67–77 (1999)Cite this article

Abstract

Lung cancer can lead to abnormalities of the actin cytoskeleton structure which may be important in transformation. In this study, we have investigated the expression of the cytoskeletal associated protein paxillin in lung cancer. Paxillin is a 68 kDa focal adhesion protein, with four tandem LIM domains at the C-terminus, involved in growth factor receptor, integrin and oncogenic signaling such as v-src, BCR/ABL, and E6 of the papilloma virus. In non-small cell lung cancer (NSCLC) cell lines, paxillin localized to the focal adhesions. The possible role of paxillin in lung cancer cells was assessed by overexpressing green fluorescence protein (GFP)-paxillin construct in two separate NSCLC cell lines (Calu-1 and H661). Over the course of 48 h, GFP-paxillin consistently caused the cells to become round and to decrease cell motility as compared to normal controls, GFP-N-terminus paxillin, or GFP-LIM transfected cells. Because some lung cancers may be quite aggressive and metastasize quickly, which may be related to the cytoskeleton, we determined the expression of paxillin in NSCLC and small cell lung cancer (SCLC) cell lines and patient tumor tissues. Expression of paxillin in NSCLC and SCLC cell lines were determined by Northern blot and Western blot analysis. The expression of paxillin was consistently low in SCLC cell lines, whereas there was paxillin expression in NSCLC cell lines. There was a variability of expression of paxillin in NSCLC tumor tissue as compared to normal lung tissue. In contrast, by immunohistochemistry, we show that there was no detectable expression of paxillin in 5/5 SCLC patients. This data suggests that absence or low level of paxillin protein expression may cause certain lung cancers, such as SCLC, to be more motile and possibly more aggressive.

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References

  • Ambros V, Chen L and Buchanan J. . 1975 Proc. Natl. Acad. Sci. USA 72: 3144–3148.

  • Bernal S, Baylin S, Shaper J, Gazdar A and Chen L. . 1983 Cancer Res. 43: 1798–1808.

  • Birge RB, Fajardo JE, Reichman C, Shoelson SE, Songyang Z, Cantley LC and Hanafusa H. . 1993 Mol. Cell. Biol. 13: 4648–4656.

    Article  CAS  Google Scholar 

  • Bockholt SM and Burridge K. . 1993 J. Biol. Chem. 268: 14565–14567.

  • Bockholt SM and Burridge K. . 1995 Cell Adhes. Commun. 3: 91–100.

  • Brown M and Cooper J. . 1996 Biochim. Biophys. Acta - Rev. Ca 1287: 121–149.

  • Carbone D and Kratzke R. . 1996 Lung cancer: Principles and practice. Mitchell J, Johnson D and Turrisi A. (eds).. Lippincott-Raven Publishers: Philadelphia. pp 107–121.

    Google Scholar 

  • Charlesworth A, Broad S and Rozengurt E. . 1996 Oncogene 12: 1337–1345.

  • Coll J, Benzeev A, Ezzell R, Fernandez J, Baribault H, Oshima R and Adamson E. . 1995 Proc. Natl. Acad. Sci. USA 92: 9161–9165.

  • Glenney J and Zokas L. . 1989 J. Cell. Biol. 108: 2401–2408.

  • Kern J and Filderman A. . 1993 Clinics Chest Med. 14: 31–41.

  • Lauffenburger D. . 1996 Nature 383: 390–391.

    Article  CAS  Google Scholar 

  • Lo S and Chen L. . 1994 Cancer Metas. Rev. 13: 9–24.

  • Lo S, Weisberg E and Chen L. . 1994 Bioessays 16: 817–823.

    Article  CAS  Google Scholar 

  • Mazaki Y, Hashimoto S and Sabe H. . 1997 J. Biol. Chem. 272: 7437–7444.

    Article  CAS  Google Scholar 

  • Parker S, Tong T, Bolden S and Wingo P. . 1997 CA Cancer J. Clin. 47: 5–27.

  • Pezzella F, Turley H, Kuzu I, Tungekar MF, Dunnill MS, Pierce CB, Harris A, Gatter KC and Mason DY. . 1997 New Engl. J. Med. 329: 690–694.

  • Prins J, De Vries E and Mulder N. . 1993 Anticancer Res. 13: 1373.

  • Rankin S, Hooshmandrad R, Claessonwelsh L and Rozengurt E. . 1996 J. Biol. Chem. 271: 7829–7834.

    Article  CAS  Google Scholar 

  • Salgia R, Avraham S, Pisick E, Li JL, Raja S, Greenfield EA, Sattler M, Avraham H and Griffin JD. . 1996a J. Biol. Chem. 271: 31222–31226.

    Article  CAS  Google Scholar 

  • Salgia R, Brunkhorst B, Pisick E, Li JL, Lo SH, Chen LB and Griffin JD. . 1995a Oncogene 11: 1149–1155.

  • Salgia R, Li J-L, Ewaniuk D, Pear W, Pisick E, Burky S, Ernst T, Sattler M, Chen L and Griffin J. . 1997 J. Clin. Invest. 100: 46–57.

    Article  CAS  Google Scholar 

  • Salgia R, Li JL, Lo SH, Brunkhorst B, Kansas GS, Sobhany ES, Sun YP, Pisick E, Hallek M, Ernst T, Tantravahi R, Chen LB and Griffin JD. . 1995b J. Biol. Chem. 270: 5039–5047.

    Article  CAS  Google Scholar 

  • Salgia R, Pisick E, Sattler M, Li JL, Uemura N, Wong WK, Burky SA, Hirai H, Chen LB and Griffin JD. . 1996b J. Biol. Chem. 271: 25198–25203.

    Article  CAS  Google Scholar 

  • Salgia R, Uemura N, Okuda K, Li JL, Pisick E, Satter M, de Jong R, Drucker B, Heisterkamp N, Chen LB. et al. 1995c J. Biol. Chem. 270: 29145–29150.

    Article  CAS  Google Scholar 

  • Sambrook J, Fritsch EF and Maniatis T. . 1990 Molecular Cloning: A Laboratory Manual, Second edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.

    Google Scholar 

  • Sattler M and Salgia R. . 1997 Cytokine & Growth Factor Rev. 8: 63–79.

  • Sattler M, Salgia R, Shrikhande G, Verma S, Uemura P, Law SF, Golemis EA and Griffin JD. . 1997 J. Biol. Chem. 272: 14320–14326.

    Article  CAS  Google Scholar 

  • Schaller MD, Otey CA, Hildebrand JD and Parsons JT. . 1995 J. Cell. Biol. 130: 1181–1187.

  • Schaller MD and Parsons JT. . 1994 Curr. Opin. Cell Biol. 6: 705–710.

  • Shapiro G, Edwards C, Kobzik L, Godleski J, Richards W, Sugarbaker D and Rollins B. . 1995 Cancer Res. 55: 505–509.

  • Sidransky D and Hollstein M. . 1996 Ann. Rev. Med. 47: 285–301.

  • Slebos R and Rodenhuis S. . 1992 J. Natl. Cancer Inst. 13: 23–29.

  • Strauss G. . 1997 Hem. Onc. Clinics North Am. 11: 409–434.

  • Tong XA and Howley PM. . 1997 Proc. Natl. Acad. Sci. USA 94: 4412–4417.

  • Turner CE. . 1994 Bioessays 16: 47–52.

    Article  CAS  Google Scholar 

  • Weisberg E, Sattler M, Ewaniuk DS and Salgia R. . 1997 Crit. Rev. Oncogenesis 8: 343–358.

  • Turner CE and Miller JT. . 1994 J. Cell. Sci. 107: 1583–1591.

  • Yamaguchi R, Maki M, Hatanaka M and Sabe H. . 1994 FEBS Letts. 356: 114–116.

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Acknowledgements

This work was partially supported by: Jose Carreras International Leukemia Foundation Fellowship FIJC-95/INT (to MS), NIH grants DK50654 (to JDG) and CA75348 (to RS).

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Authors and Affiliations

  1. Division of Hematologic Oncology, Dana-Farber Cancer Institute and Harvard Medical School, 44 Binney Street, Boston, 02115, Massachusetts, USA

    Ravi Salgia, Jian-Liang Li, Darren S Ewaniuk, Martin Sattler, Wen-Che Chen, Evan Pisick & James D Griffin

  2. Division of Cellular and Molecular Biology, Dana-Farber Cancer Institute and Harvard Medical School, 44 Binney Street, Boston, 02115, Massachusetts, USA

    You-Bin Wang & Lan Bo Chen

  3. Division of Neoplastic Disease Mechanisms, Dana-Farber Cancer Institute and Harvard Medical School, 44 Binney Street, Boston, 02115, Massachusetts, USA

    Geoffrey I Shapiro & Barrett J Rollins

  4. Division of Thoracic Oncology and Adult Oncology, Dana-Farber Cancer Institute and Harvard Medical School, 44 Binney Street, Boston, 02115, Massachusetts, USA

    Ravi Salgia, William Richards, Geoffrey I Shapiro, Barrett J Rollins & David J Sugarbaker

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  1. Ravi Salgia
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Salgia, R., Li, JL., Ewaniuk, D. et al. Expression of the focal adhesion protein paxillin in lung cancer and its relation to cell motility. Oncogene 18, 67–77 (1999). https://doi.org/10.1038/sj.onc.1202273

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