Abstract
Anaplastic large-cell lymphoma (ALCL) is a distinct biological and cytogenetic entity with a broad spectrum of morphological features (common type, small-cell variant and lymphohistiocytic variant). Few cell lines of ALCL are available and they all originate from primary tumors demonstrating the common type morphology (ie large-sized lymphoma cells). We established a new ALCL cell line (COST) from the peripheral blood of a patient with a small-cell variant of ALCL, at diagnosis. Cells growing in vitro and in SCID mice consisted of two populations, that is, small- and large-sized cells as seen in the patient's tumor. Both large and small malignant cells were positive for CD43/MT1 T-cell associated antigen, perforin, granzyme B and TIA-1, but negative for CD2, CD3, CD5, CD7, CD4 and CD8 antigens. Standard cytogenetic studies as well as multiplex FISH confirmed the presence of the canonical t(2;5)(p23;q35) translocation, but also revealed additional numerical and structural abnormalities. The COST cell line is the first ALCL small-cell variant cell line, and thus provides a potentially useful tool for further functional and molecular studies that should improve our understanding of the small-cell variant of ALCL, which is more frequently complicated by a leukemic phase.
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Acknowledgements
We thank Michel March for his excellent technical work performing immunohistochemistry, Daniel Roda and Jeanine Boye. We acknowledge the paediatrician, Dr Alain Robert for providing us clinical data. This work was supported by the ‘Ligue Contre le Cancer’, Comités de la Haute-Garonne et de l'Aveyron.
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Lamant, L., Espinos, E., Duplantier, M. et al. Establishment of a novel anaplastic large-cell lymphoma-cell line (COST) from a ‘small-cell variant’ of ALCL. Leukemia 18, 1693–1698 (2004). https://doi.org/10.1038/sj.leu.2403464
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DOI: https://doi.org/10.1038/sj.leu.2403464
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