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An autosomal genomic scan for loci linked to plasma leptin concentration in Pima Indians

International Journal of Obesity volume 24, pages 559–565 (2000)Cite this article

Abstract

OBJECTIVE: To identify chromosomal regions linked to plasma leptin concentrations.

DESIGN: Autosomal genome-wide scan, including 516 microsatellite markers. Sib-pair (Haseman–Elston) and variance components methods were used to assess genetic linkage.

SUBJECTS: 770 Pima Indians comprising 239 nuclear families (for a total of 1199 sibling-pairs).

MEASUREMENTS: Plasma leptin concentrations and body mass index (BMI), adjusted for age and sex.

RESULTS: The strongest evidence for linkage with plasma leptin concentration was on chromosome 6p logarithm of odds (LOD)=2.1 by variance components analysis). There was no evidence for linkage to BMI in this region. Additional regions with marginal evidence for linkage to plasma leptin concentration (LOD ≥ 1.0) were detected on chromosomes 3, 11, 13, 15 and 16.

CONCLUSIONS: The results suggest that a locus on chromosome 6p influences plasma leptin concentrations. Replication studies are needed to exclude the possibility that linkage has been falsely detected.

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Acknowledgements

We thank the members of the Gila River Indian Community for participating in these studies. We thank also Margery Nicolson and colleagues at Amgen Inc., Thousand Oaks, California who performed the assays for plasma leptin concentrations. The pedigree data and DNA were collected by Vickie Andre, Linda Phillips, Sonja Antone and other staff of the Diabetes and Arthritis Epidemiology Section. Microsatellite typings were performed by the Marshfield Clinic and Glaxo Wellcome Inc., with assistance from Derek Traughber, Gopi Gopalam and Madhu Sethi, and Don Holt prepared the genetic maps. Assistance in implementing the variance-components analysis was provided by John Blangero and Laura Almasy.

Some of the results of this paper were obtained with the program package SAGE, which is supported by US Public Health Service Resource Grant 1 P41 RR03655 from the National Center for Research Resources.

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Authors and Affiliations

  1. Phoenix Epidemiology and Clinical Research Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Phoenix, 85016, AZ, USA

    K Walder, RL Hanson, S Kobes, WC Knowler & E Ravussin

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  1. K Walder
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Correspondence to RL Hanson.

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Walder, K., Hanson, R., Kobes, S. et al. An autosomal genomic scan for loci linked to plasma leptin concentration in Pima Indians. Int J Obes 24, 559–565 (2000). https://doi.org/10.1038/sj.ijo.0801197

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