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Stem Cell Sources

Ancestim (recombinant human stem cell factor, SCF) in association with filgrastim does not enhance chemotherapy and/or growth factor-induced peripheral blood progenitor cell (PBPC) mobilization in patients with a prior insufficient PBPC collection

Bone Marrow Transplantation volume 34, pages 683–691 (2004)Cite this article

Summary:

Up to a third of autologous transplantation candidates fail to mobilize hematopoietic progenitors into the peripheral blood with chemotherapy and/or growth factor treatment, thus requiring innovative mobilization strategies. In total, 20 cancer patients unable to provide adequate PBPC products after a previous mobilization attempt were treated with ancestim (20 μg/kg/day s.c.) and filgrastim (10 μg/kg/day s.c.). In 16 patients, the pre-study mobilization was with filgrastim alone. Eight patients underwent single large volume leukapheresis (LVL) and 12 multiple standard volume leukaphereses (SVL) in both mobilizations. Pairwise comparison of peripheral blood CD34+ cell concentrations on the day of first leukapheresis failed to document synergism – median CD34+/μl of 3.2 (<0.1 to 15.4) and 4.5 (1–28.56) for the pre-study and on-study mobilizations (P=0.79, sign test), and 4.2 (<0.1–15.4) and 5 (1–28.56), respectively, for the 16 patients previously mobilized with filgrastim alone (P=1, sign test). The number of CD34+ cells/kg collected per unit of blood volume (BV) processed was similar in both mobilizations – median 0.1 × 106/kg/BV and 0.09 × 106/kg/BV, respectively (P=1, sign test). In this phase II study, the combination of ancestim and filgrastim did not allow adequate PBPC mobilization and collection in patients with a previous suboptimal PBPC collection.

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Authors and Affiliations

  1. Bone Marrow Transplantation Unit, Instituto Português de Oncologia de Francisco Gentil, Lisboa, Portugal

    M G da Silva, A Machado, N Miranda, F Leal da Costa & J L Passos-Coelho

  2. Bone Marrow Transplantation Unit, Instituto Português de Oncologia de Francisco Gentil, Porto, Portugal

    P Pimentel, A Carvalhais, I Barbosa, F Campilho, S R Sousa, A Campos & C P Vaz

  3. AMGEN, Portugal

    J Antas

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  1. M G da Silva
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Correspondence to M G da Silva.

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da Silva, M., Pimentel, P., Carvalhais, A. et al. Ancestim (recombinant human stem cell factor, SCF) in association with filgrastim does not enhance chemotherapy and/or growth factor-induced peripheral blood progenitor cell (PBPC) mobilization in patients with a prior insufficient PBPC collection. Bone Marrow Transplant 34, 683–691 (2004). https://doi.org/10.1038/sj.bmt.1704602

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