Abstract
Background:
The combination of temozolomide (TMZ) and irinotecan is a regimen used in neuroblastoma patients with recurrent disease. O6-methylguanine-DNA methyltransferase (MGMT) may have a function in resistance to TMZ. Using neuroblastoma pre-clinical models, we determined whether the inhibition of MGMT by O6-benzylguanine (O6-BG) could enhance the anti-tumour activity of TMZ and irinotecan.
Methods:
The cytotoxicity of TMZ and irinotecan, either alone or in combination, was measured in five neuroblastoma cell lines in the presence or absence of O6-BG with a fluorescence-based cell viability assay (DIMSCAN). Anti-tumour activity was measured in three neuroblastoma xenograft models.
Results:
MGMT mRNA and protein were expressed in 9 out of 10 examined cell lines. Pretreatment of cells with 25 μ M O6-BG decreased MGMT protein expression and enhanced The TMZ cytotoxicity by up to 0.3–1.4 logs in four out of five tested cell lines. TMZ (25 mg kg−1 per day for 5 days every 3 weeks for four cycles) did not significantly improve mice survival, whereas the same schedule of irinotecan (7.5 mg kg−1 per day) significantly improved survival (P<0.0001) in all three xenograft models. Combining O6-BG and/or TMZ with irinotecan further enhanced survival.
Conclusion:
Our in vitro and in vivo findings suggest that irinotecan drives the activity of irinotecan and TMZ in recurrent neuroblastoma. Inhibitors of MGMT warrant further investigation for enhancing the activity of regimens that include TMZ.
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Main
Neuroblastoma is the most common extracranial solid tumour of childhood. Myeloablative chemotherapy followed by hematopoietic stem-cell transplantation and then 13-cis retinoic acid (Matthay et al, 1999) or most recently anti-GD2 antibody and cytokines (Yu et al, 2009) improve survival of children with high-risk neuroblastoma, but many patients still die of progressive disease. Therefore, the development of new therapies is a priority.
Irinotecan, a topoisomerase I inhibitor, is a semi-synthetic water-soluble analogue of camptothecin, a pro-drug that undergoes de-esterification to an active metabolite, SN-38. Irinotecan, as a single agent, has shown activity in vitro (Keshelava et al, 2000) and in xenograft neuroblastoma models (Vassal et al, 1996; Thompson et al, 1997; Furman et al, 1999), and demonstrated some objective responses in neuroblastoma patients treated with various doses and schedules (Furman et al, 1999; Blaney et al, 2001; Kushner et al, 2005; Bomgaars et al, 2006, 2007). Preclinical data from rhabdomyosarcoma and neuroblastoma xenograft models have demonstrated schedule-dependent activity for irinotecan, with superior activity seen when using protracted low-dose administration (Houghton et al, 1995).
Temozolomide (TMZ), a DNA methylating imidazole tetrazinone, inhibits cell growth in neuroblastoma xenografts (Middlemas et al, 2000) and has been shown to have activity in high-risk neuroblastoma patients (De et al, 2006; Rubie et al, 2006). It has been hypothesised that the cytotoxic activity of TMZ is mediated through reactive O6-methylguanine in DNA (Tisdale, 1987); thus, inhibition of O6-methylguanine-DNA methyltransferase (MGMT), a DNA repair protein at the O6-guanine position, may increase the cytotoxicity of TMZ against neuroblastomas (Wagner et al, 2007b). O6-benzylguanine (O6-BG) is a potent suppressor of MGMT activity, acting as a substrate for MGMT (Dolan et al, 1990).
Irinotecan is commonly used in combination with TMZ to treat recurrent high-risk neuroblastomas (Wagner et al, 2009) because of their synergistic interaction in xenograft models (Houghton et al, 2000), and their activity in patients with Ewing sarcoma (Wagner et al, 2007a) and high-grade glioma (Reardon et al, 2005). Clinically, these agents are administered sequentially, starting with TMZ, because of the schedule-dependent activity demonstrated in xenograft models. It is presumed that the placement of an adduct at the O6 position of guanine by an alkylating agent, such as BCNU or TMZ, is critical for achieving synergistic activity with irinotecan (Castellino et al, 2000).
The purpose of the current study was to assess the activity of the TMZ and irinotecan combination in neuroblastoma pre-clinical models and to determine whether inhibition of MGMT by O6-BG significantly enhanced the anti-tumour activity of the combination.
Materials and methods
Drugs and chemicals
Both TMZ and O6-BG were obtained from the Drug Synthesis and Chemistry Branch, Developmental Therapeutics Program, National Cancer Institute (Bethesda, MD, USA). SN-38 was purchased from Abatra Technology Co., Ltd, and irinotecan was purchased from the pharmacy at Childrens Hospital Los Angeles. Fluorescein diacetate was purchased from Eastman Kodak Company (Rochester, NY, USA), and eosin Y was from Sigma Chemical Co. (St Louis, MO, USA). Antibodies were purchased from various manufacturers: anti-MGMT monoclonal MT 3.1 Ab-1 from NeoMarkers (Fremont, CA, USA), caspase-3 (Cat# 9665) and phospho-H2AX (Ser 139, Cat# 9718) rabbit monoclonal antibody from Cell Signaling Technology (Danvers, MA, USA) and F7-26 from Chemicon International (Temecula, CA, USA).
Cell Lines
We used four drug-sensitive (SMS-SAN, CHLA-42, CHLA-15 and SMS-KCNR) and seven multidrug-resistant (CHLA-171, CHLA-136, SK-N-RA, SK-N-BE(2), CHLA-90, CHLA-119 and CHLA-172) cell lines. CHLA-136 and SK-N-RA cell lines carry wild type and transcriptionally active TP53 (Keshelava et al, 2001). SK-N-BE(2), CHLA-90, CHLA-119 and CHLA-172 carry mutant and transcriptionally inactive TP53 (Keshelava et al, 2001). CHLA-171 carries wild type, but transcriptionally inactive TP53, likely because of the MDM2 protein overexpression (Keshelava et al, 2001).
SMS-KCNR cells were grown in RPMI-1640 supplemented with 10% heat-inactivated fetal bovine serum (Omega Scientific, Tarzana, CA, USA). All other cells were grown in Iscove's Modified Dulbecco's Medium (Biowhittaker, Walkersville, MD, USA) supplemented with 20% fetal bovine serum, 3 mM L-Glutamine (Mediatech, Inc., Herndon, VA, USA) and 0.1% premium ITS culture supplement containing 5 μg ml−1 insulin, 5 μg ml−1 transferrin and 5 ng ml−1 selenious acid (VWR Scientific, West Chester, PA, USA). The cell lines were cultured without antibiotics at 37°C in a humidified incubator containing 95% air and 5% CO2 atmosphere. All cell lines tested negative for mycoplasma. Cell lines were not selected for drug resistance in vitro. The identities of all cell lines were confirmed by a short tandem repeat assay (Masters et al, 2001).
TaqMan RT-PCR
TaqMan real-time reverse transcription-PCR (RT-PCR) was used to quantify RNA with the Sequence Detection System (ABI Prism model 7700; Applied Biosystems, Foster City, CA, USA). The probe and primer for MGMT gene were purchased from Applied Biosystems (Cat# Hs00172470).
Reactions were performed with 150 ng of RNA in the Master Mix prepared from the kit, TaqMan one-step RT-PCR Master Mix Reagents (Applied Biosystems, Branchburg, NJ, USA), in a final volume of 25 μl. Three replicates for each reaction were plated into 96-well plates. RT-PCR assay was performed under the following conditions: 2 min at 50°C, 10 min at 95°C, 40 cycles of denaturation at 95°C for 15 s and annealing/extension at 60°C for 1 min. The RNA of each sample was normalised to the level of GAPDH.
Cytotoxicity assay
We determined the cytotoxicity of O6-BG, irinotecan and TMZ, as single drugs or in combination, in the CHLA-15, CHLA-42, SMS-KCNR, CHLA-136 and CHLA-90 cell lines by DIMSCAN (Keshelava et al, 2005; Frgala et al, 2007), a semiautomatic fluorescence-based digital image microscopy system that quantifies viable cells in tissue culture multi-well plates on the basis of their selective accumulation of fluorescein diacetate. DIMSCAN is capable of measuring cytotoxicity over a four-log dynamic range by quantifying total fluorescence per well, which is proportional to the number of viable, clonogenic cells after eliminating background fluorescence with digital thresholding and eosin Y quenching (Keshelava et al, 2005; Frgala et al, 2007).
Briefly, cell lines were seeded into 96-well plates in 150 μl of complete medium (1500–3500 cells per well) and incubated overnight. O6-BG (25 μ M) in 50 μl of complete medium was added to each well. After an, additional, 24-h incubation, TMZ (0–50 μg ml−1) and SN-38, an active in vitro metabolite of irinotecan (0–20 ng ml−1), were added. We chose a range of concentrations for TMZ and SN-38 that would encompass drug levels active in pre-clinical models and clinically relevant. Protracted administration of low-dose irinotecan in children resulted in a Cmax of ∼20 ng ml−1 (Ma et al, 2000), a level that was cytotoxic to neuroblastoma cells (Keshelava et al, 2000). The clinically achievable level of TMZ in children is 13±2.8 μg ml−1 (Horton et al, 2007) and in xenograft models, TMZ Cmax is 36 μg ml−1 (Houghton et al, 2000). Each drug concentration was tested in 12 replicate wells. Cell lines were incubated in the presence of these agents for 72 h, after which fluorescein diacetate in 50 μl of 0.5% eosin Y (final concentration of fluorescein diacetate=10 μg ml−1) was added to each well and cells were incubated for an additional 25 min at 37°C. Total fluorescence was then measured with the use of DIMSCAN as previously described (Keshelava et al, 2005; Frgala et al, 2007), and results were expressed as surviving fractions of treated cells compared with control cells that were exposed to vehicle solution alone.
In addition to simultaneous administration of TMZ and SN-38, we also tested sequential administration of these drugs in the CHLA-15, SMS-KCNR and CHLA-90 cell lines. In these experiments, TMZ was added to cells 3 h before irinotecan.
ssDNA damage measurements with F7-26 antibody
Cells were treated with O6-BG (25 μ M, 24 h), TMZ (50 μg ml−1, 6 h), SN-38 (20 ng ml−1, 4 h) or their combinations, and analysed after overnight fixation in methanol–PBS (6:1). Staining with F7-26 monoclonal antibody was performed according to the manufacturer's instructions. Flow cytometry was performed on a BD LSR II flow cytometer (BD Biosciences, San Jose, CA, USA) and data were acquired and analysed using BD FACSDiva software.
Immunoblot analysis of protein expression
Cells from three drug-sensitive cell lines (SMS-KCNR, SMS-SAN and CHLA-42) and seven multidrug-resistant cell lines (CHLA-171, CHLA-136, SK-N-RA, SK-N-BE(2), CHLA-90, CHLA-119 and CHLA-172) were grown to 70% confluence in 75 cm2 flasks and then lysed in immunoprecipitation assay buffer (50 mM NaCl, 50 mM Tris (pH 7.4), 1% Triton X-100, 1% sodium deoxycholate, 0.1% sodium dodecyl sulphate). Total protein (30 μg per lane) was fractionated on 10% tris-glycine precast gels (Novex, San Diego, CA, USA), transferred to nitrocellulose membrane (Protran, Keene, NH, USA), and probed with primary antibodies at a 1 : 1000 dilution, followed by incubation with an HRP-conjugated secondary antibody. The membranes were incubated with SuperSignal West Pico chemiluminescent substrate (Pierce Biotechnology, Inc., Rockford, IL, USA), and protein: antibody complexes were detected by autoradiography (Denville Scientific, Inc., Metuchen, NJ, USA).
H2AX expression, phosphorylated at Ser 139, was assessed in CHLA-15 cells for characterisation of DNA double strand breaks. Similar doses and schedules were tested as with F7-26 monoclonal antibody staining.
Animals
Athymic (nu/nu) female mice, 6–7 weeks old (Harlan Laboratories, Inc., San Diego, CA, USA) were used in the study. Mice were housed in an air-conditioned room and fed the standard laboratory chow. All procedures were performed in compliance with the Childrens Hospital Los Angeles Institutional Animal Care and Use Committee-approved protocols.
Mouse models of mass disease
We used the SMS-KCNR, CHLA-136 and CHLA-119 neuroblastoma cell lines for the xenograft experiments. These cell lines were chosen to represent neuroblastomas that were: (a) drug-sensitive (SMS-KCNR), (b) multidrug-resistant with wild-type and transcriptionally active TP53 (CHLA-136) and (c) multidrug-resistant with mutant and transcriptionally inactive TP53 (CHLA-119) (Keshelava et al, 2001). Treatments began when tumours reached 200 mm3. Tumour measurements were made twice a week. Once tumour reached 1500 mm3, mice were humanely sacrificed.
Treatment protocol
Drugs were given as follows: O6-BG (30 mg kg−1 delivered through intraperitoneal injections) was followed 1 h later by TMZ (25 mg kg−1 given by oral gavage) and 1 h later by irinotecan (7.5 mg kg−1 given by tail vein injection). O6-BG was dissolved in 4% DMSO, 30% PEG-400 and 66% saline, TMZ was dissolved in water, and irinotecan in saline. Control mice were administered sterile carrier solutions. Mice were treated on a 1-week on (5 days)-2 weeks off schedule for four cycles. The doses of administered drugs were chosen following determination of the maximum tolerated dose. The administered drugs at the doses reported here were well tolerated; no weight loss, diarrhea or abnormal behaviour was observed. White blood cell count was not monitored.
Data analysis
IC90 (drug concentrations that were cytotoxic or growth inhibitory for 90% of the cell population), and dose reduction index values were calculated using CalcuSyn software (Biosoft, Cambridge, UK) (Chou and Talalay, 1984).
The association between log-transformed RT-PCR values for MGMT RNA expression and immunoblot values for MGMT protein expression was assessed by calculating the Pearson's correlation coefficient. All P-values are two-sided. The computations of the Pearson's correlation coefficient were performed with the use of STATA software (version 9.0; College Station, TX, USA). A P-value of less than or equal to 0.05 was considered statistically significant.
The effect of TMZ pre-treatment was studied by comparing dose–response curves of the TMZ and irinotecan combination, in the presence or absence of O6-BG, with those drugs administered simultaneously or sequentially. The difference in the cytotoxic effect was examined by comparing the area between the dose–response curve and the 100% survival line. Statistical analysis assumed that the difference between the untreated controls and the highest drug concentration was 1. The fixed ratio design of the cytotoxicity experiments allowed the concentration increments to be quantitated at 1/8, 1/4, 1/2 and 1. The area between the survival curves for each treatment group and the 100% survival line was viewed as the sum of areas of four trapezoids, computed as:
where \(\overline{F}\)jk is the mean fraction of logs of cell kill after treatment at the jth dose (0th, 1st, 2nd, 3rd and 4th drug conditions). The linear combination:
then tested the difference in treatment effects between two dose–response curves (designated as i and j). Data were analysed with software SAS Version 9.1 (College Station, TX, USA).
For xenograft studies, the main effects of the three drugs (O6-BG, TMZ and irinotecan) on the length of survival of mice, as well as their second- and third-order interactions (e.g., enhancement of the effect of one drug with addition of another) were evaluated using censored regression models as implemented in the CNREG routine of STATA 9.0 (StataCorp LP, College Station, TX, USA). Days of survival were assumed to have a lognormal distribution, and the days were transformed to the natural log scale before analyses were carried out. O6-BG, TMZ and irinotecan, as well as their second- and third-order interaction terms were included in the initial regression model. For main drug effects, a P-value less than or equal to 0.05 was considered statistically significant. For test of interaction effect between drugs (e.g., enhancement in the effect of one drug with addition of another), a P-value less than or equal to 0.01 was considered statistically significant. P-values from likelihood ratio tests are presented. To compare the length of survival between any two groups of mice, a censored regression model was first fit using data from all the treatment groups with the CNREG routine, then difference in mice survival between two treatment groups was evaluated by testing the appropriate linear combinations using the STATA LINCOM command. Data from the three cell lines was analysed separately. All P-values reported were two-sided.
Results
MGMT RNA and protein expression
Around 10 neuroblastoma cell lines (SMS-SAN, SMS-KCNR, CHLA-42, CHLA-171, CHLA-136, SK-N-RA, SK-N-BE(2), CHLA-90, CHLA-119 and CHLA-172) were tested for MGMT RNA and protein expression by TaqMan RT-PCR and immunoblotting, respectively (Figure 1A and B). All cell lines, except CHLA-90, expressed MGMT. MGMT RNA expression correlated significantly with MGMT protein expression (r=0.67, P<0.03). Thus, MGMT is expressed in neuroblastomas and may be a pharmacological target for therapeutic regimens that include TMZ, as previously suggested (Wagner et al, 2007b).
Cytotoxicity of O6-BG, TMZ and irinotecan in neuroblastoma cell lines
Next, we examined whether O6-BG can increase the activity of TMZ or the TMZ and irinotecan combination in neuroblastomas using three drug-sensitive (CHLA-15, CHLA-42 and SMS-KCNR) and two multidrug-resistant (CHLA-90 and CHLA-136) cell lines, including the one that does not express MGMT (CHLA-90) (Figures 2 and 3). For these in vitro experiments, O6-BG was tested at 25 μ M, a concentration chosen on the basis of the studies demonstrating significant reduction of MGMT enzymatic activity in vitro by O6-BG at 10 μ M (Bacolod et al, 2002). Depletion of MGMT protein by 75 μ M O6-BG has also been demonstrated (Murakami et al, 2007). Using western blotting, we confirmed reduced protein expression following treatment with 25 μ M O6-BG in the SMS-KCNR and CHLA-136 cell lines (data not shown).
Figure 2 shows the dose–response curves obtained from the DIMSCAN assay. We calculated the concentrations that were growth inhibitory or cytotoxic for 90% of treated cells (IC90) for single drugs or drug combinations using the dose–response curves for TMZ, SN-38 and TMZ and SN-38 in the presence or absence of 25 μ M O6-BG.
The single-agent IC90 values for TMZ ranged from 27 to >50 μg ml−1 (Table 1). Addition of 25 μ M O6-BG enhanced the growth inhibitory effect of TMZ by up to 1.4 logs in CHLA-15, 0.6 logs in CHLA-42, 0.9 logs in SMS-KCNR and 0.3 logs in CHLA-136 cells (Figure 2). The single-agent IC90 values for SN-38 ranged from <2.5 to >20 ng ml−1 (Table 1). Addition of 25 μ M O6-BG marginally enhanced growth inhibitory effect of SN-38 in the CHLA-42 cell line. The TMZ and SN-38 anti-neuroblastoma activity was tested at a fixed ratio of 2.5 : 1. The growth inhibitory activity of the combination was SN-38 driven in all tested lines. Addition of 25 μ M O6-BG increased TMZ and SN-38-mediated growth inhibition by up to 0.6 logs in CHLA-42 and 0.5 logs in SMS-KCNR cell lines (Figure 2).
As TMZ is given before irinotecan to neuroblastoma patients, we examined whether administration of TMZ 3 h before SN-38 exposure would result in higher cytotoxicity in representative neuroblastoma cell lines: CHLA-15, SMS-KCNR and CHLA-90. Comparison of the dose–response curves of TMZ and SN-38 in the presence or absence of O6-BG, with the drugs administered simultaneously or sequentially, did not reach statistical significance (Figure 3).
DNA damage in vitro
Single- or double-strand (ss or ds) DNA breaks caused by the tested drugs and drug combinations were measured in a representative neuroblastoma cell line (CHLA-15), selected for its propensity to grow in single-cell suspension that does not clump excessively. F7-26 monoclonal antibody staining to single-stranded DNA was used to quantify ssDNA damage (Figure 4A and B). We have previously shown that this method distinguishes ssDNA breaks caused by exogenous stimuli from DNA strand breaks resulting from apoptosis (Grigoryan et al, 2007). Phosphorylation of the H2A histone variant, H2AX, at serine-139 was used to quantify dsDNA damage (Figure 4C).
SN-38 induced more prominent single-strand (4.3-fold higher F7-26 staining) or double-strand (84-fold higher expression of pS139-H2AX) DNA damage, compared with TMZ (ssDNA=two-fold, dsDNA=11-fold) or O6-BG (ssDNA=1 and dsDNA=4). Significantly more ssDNA damage was achieved by any of the two drug combinations (O6-BG and TMZ, O6-BG+SN-38 and TMZ and SN-38, P-values <0.001) relative to simple addition of single drug actions as measured by linear regression analysis. Synergistic induction of ssDNA breaks was not demonstrated for the triple drug combination. Thus, the data show that increased DNA damage by the two- and three-drug combinations results mostly from the breakage induced by SN-38, although TMZ and/or O6-BG further enhanced DNA breaks.
Activity in the mouse xenograft models
First, we confirmed that the planned dosing schedule achieved the desired pharmacodynamic effect on the xenograft tumours. SMS-KCNR mice were treated with O6-BG or/and TMZ for 1 week and sacrificed. Tumour tissues were obtained and MGMT depletion by O6-BG was demonstrated by immunoblotting (Figure 5A), whereas induction of apoptosis was verified by cleavage of caspase 3 (Figure 5B).
Formal factorial design was applied to our xenograft studies for comprehensive assessment of contributions of single drugs or two-drug combinations to the anti-tumour activity obtainable by the O6-BG, TMZ and irinotecan combination (Table 2).
TMZ as a single agent did not have any significant effect on survival of either SMS-KCNR (P=0.21), CHLA-136 (P=0.81) or CHLA-119 (P=0.9) xenografts; however, addition of O6-BG resulted in significant evidence of enhancement of TMZ effect in SMS-KCNR xenografts (P=0.002). This enhancement was marginally significant in CHLA-119 (P=0.018) xenografts.
O6-BG, as a single agent, significantly improved survival of the CHLA-136 xenografts (P=0.007). When administered as single agents, TMZ and O6-BG did not show significant difference in mean survival days in SMS-KCNR (P=0.29) or CHLA-119 (P=0.13) xenografts, but showed significant difference in CHLA-136 (P=0.028).
Irinotecan delivered the most activity among the treated groups (Figure 6). As a single agent, irinotecan significantly improved the survival rate (P<0.0001) in all three xenograft models. Addition of TMZ (Figure 6) or O6-BG did not significantly improve irinotecan activity. In contrast, addition of O6-BG to the TMZ and irinotecan combination significantly increased survival days of SMS-KCNR (P=0.017), CHLA-136 (P=0.033) and CHLA-119 (P=0.017) xenografts (Figure 6). Notably, 60% of the CHLA-119 xenografts were still alive at 400 days, indicating a significant effect on survival of the O6-BG, TMZ and irinotecan combination.
Discussion
The combination of TMZ, a DNA methylating agent, with irinotecan, a topoisomerase I inhibitor, had emerged as a promising regimen for recurrent neuroblastoma (Kushner et al, 2006; Wagner et al, 2009). However, recent evidence suggests that the effectiveness of TMZ in neuroblastomas can be diminished by MGMT-mediated DNA damage repair (Middlemas et al, 2000; Wagner et al, 2007b). MGMT is frequently expressed in neuroblastoma tumours and cell lines (Middlemas et al, 2000; Wagner et al, 2007b). MGMT methylation in the promoter region has also been examined in neuroblastomas as promoter hypermethylation is associated with decreased MGMT expression. In those studies, promoter hypermethylation ranged from 0 to 27% in the examined tumours and cell lines (Harada et al, 2002; Gonzalez-Gomez et al, 2003; Lazcoz et al, 2007). Depletion of MGMT activity by the selective inhibitor, O6-BG, reportedly enhanced the cytotoxicity of TMZ in human brain tumour (Bobola et al, 1996) and melanoma (Wedge et al, 1997) xenografts. O6-BG also enhanced the anti-tumour activity of the TMZ and irinotecan combination in a model of disseminated neuroblastoma (Wagner et al, 2007b) and glioma xenograft models (Friedman et al, 2002).
We undertook this large-scale study to formally assess the anti-tumour activities of O6-BG, TMZ and irinotecan, as well as their second- and third-order interactions (such as enhancement of one-drug effect with addition of another) in neuroblastoma pre-clinical models. To our knowledge, such extensive assessment has not been carried out previously.
In our study, 10 neuroblastoma cell lines were examined for MGMT RNA and protein expression. All expressed MGMT, except the multidrug-resistant cell line, CHLA-90, which carries a TP53 mutation (Keshelava et al, 2001). This is consistent with a report showing that promoter methylation of the MGMT gene (which results in low or absent MGMT expression) is associated with an increased frequency of TP53 mutations (Nakamura et al, 2001).
We have evaluated the anti-neuroblastoma activity of O6-BG, TMZ and irinotecan in vitro and in vivo. Drug levels used for O6-BG decreased MGMT protein expression both in vitro (data not shown) and in vivo (Figure 5A). Our in vitro data confirmed that O6-BG enhanced the cell sensitivity to TMZ. Pre-treatment with 25 μ M O6-BG sensitised four out of the five tested neuroblastoma cell lines to TMZ by 0.3–1.4 logs (Figure 2); sensitising effect was not demonstrated in the highly drug-resistant CHLA-90 cells that lack MGMT expression. In xenografts, O6-BG as a single agent had a significant effect on the survival of CHLA-136 tumour-bearing mice (P=0.007). O6-BG significantly enhanced TMZ activity in SMS-KCNR (P=0.002) and marginally in CHLA-119 (P=0.018) mice.
Temozolomide, either alone (Rubie et al, 2006) or in combination with irinotecan (Wagner et al, 2009), is being actively investigated as a possible first-line regimen for recurrent neuroblastomas. In our study, single agent TMZ did not have a significant effect on the survival of either SMS-KCNR, CHLA-136 or CHLA-119 xenografts at the administered dose and schedule. We carefully selected the drug levels for our in vitro (0–50 μg ml−1) and xenograft experiments (25 mg kg−1 per day), on the basis of the previously reported pre-clinical and clinical pharmacokinetic data of TMZ. The pharmacokinetic studies by Stevens et al, and Houghton et al, conducted in mice, demonstrated that oral administration of 20 mg kg−1 or 66 mg kg−1 temozolomide resulted in a Cmax of 19.6 μg ml−1 (Stevens et al, 1987) or 36 μg ml−1 (Houghton et al, 2000), respectively. The TMZ Cmax levels achievable in children are in the range of 5.6±1.8 μg ml−1 (Baruchel et al, 2006) to 13±2.8 μg ml−1 (Horton et al, 2007). Thus, administration of 25 mg kg−1 per day TMZ in mice would result in a systemic exposure well within the clinically relevant drug levels. By demonstrating caspase 3 activation, a hallmark of apoptosis (Figure 5B), in SMS-KCNR xenografts, we confirmed the suitability of the administered drug dose.
Irinotecan, as a single-agent, had substantial activity in vitro in four neuroblastoma cell lines, achieving 1.1–2.8-log cytotoxicity at 20 ng ml−1 (achievable in patients, Vassal et al, 2003), and in our xenograft models. Our results are in agreement with other pre-clinical investigations of irinotecan that demonstrated activity against neuroblastomas (Komuro et al, 1994; Vassal et al, 1996; Thompson et al, 1997; Furman et al, 1999). However, phase II clinical trials of single-agent irinotecan have only shown limited activity in neuroblastoma patients (Bomgaars et al, 2007; Vassal et al, 2008). Such divergence could be attributed to the disparate metabolism of irinotecan into its active metabolite SN-38 in different species. It is estimated that mouse liver and kidney carboxylesterase more actively hydrolyses irinotecan than either human carboxylesterase1 (hCE1) (173 vs 2.5 pmol mg−1 min−1 activity) (Xie et al, 2003) or hCE2, a more potent activator of irinotecan than human carboxylesterase1 (Humerickhouse et al, 2000). Nonetheless, the strong anti-neuroblastoma activity achieved by irinotecan in all our three xenograft models warrants further testing of irinotecan with additional agents to identify synergistic combinations against recurrent neuroblastomas.
In contrast to previous pre-clinical investigations of TMZ and irinotecan in neuroblastoma xenograft models (Houghton et al, 2000), our studies did not demonstrate synergy between these drugs. The difference may be attributable to the use in the studies by Houghton et al of dose levels of irinotecan and TMZ that were unlikely to induce complete responses when administered alone. In our study, standardised dose-schedule for irinotecan and TMZ were used to mimic the clinical application of O6-BG, TMZ and irinotecan in children. In addition, the 5-day schedule for irinotecan instead of the (dx5(x2)) schedule used by Houghton et al may have also contributed to the different responses seen in these two pre-clinical studies.
Previous studies have examined the effect of O6-BG on the anti-tumour activity of the TMZ and irinotecan combination in neuroblastomas (Wagner et al, 2007b) and in gliomas (Friedman et al, 2002; Quinn et al, 2009). A recent phase I clinical trial in adults with recurrent malignant glioma evaluated the combination of O6-BG, TMZ and irinotecan, administered once on 21-day schedule (Quinn et al, 2009). Although the primary goal of the study was to define the maximum tolerated dose and dose-limiting toxicity of the evaluated drugs, it did not demonstrate any responses. The lack of responses was hypothesized to be due to subtherapeutic doses or timing of administered doses. Our in vivo studies, conducted in a mouse model of mass disease, demonstrated significantly improved survival for xenografts treated with the TMZ and irinotecan combination in the presence of O6-BG, in agreement with the findings by Wagner et al in a mouse model of metastatic disease (Wagner et al, 2007b). Our data suggest that the enhanced anti-neuroblastoma activity by the triple combination resulted from significant irinotecan activity together with the added benefit of statistically significant O6-BG (in CHLA-136) or O6-BG and TMZ (in SMS-KCNR and CHLA-119) activities. This is confirmed by the DNA studies where single- or double-stranded DNA damage was mostly due to SN-38 and further enhanced with the addition of TMZ and/or O6-BG, emphasising the critical cytotoxic effect of irinotecan in the combination. Moreover, we observed improved survival at 400 days of the multidrug-resistant xenograft CHLA-119 in contrast to the study by Wagner et al, in which the apparent advantage of adding O6-BG to TMZ and irinotecan seen at 100 days was lost at 300 days. The difference between these two studies can be explained by the use of four cycles of therapy in our study rather than two as employed by Wagner et al, or it may be attributable to the utilisation of the different strain of mice.
Our extensive in vitro and in vivo investigations using formal factorial design allowed us to convincingly demonstrate in neuroblastoma pre-clinical models that (a) irinotecan administered on a protracted schedule has remarkable anti-tumour activity; (b) MGMT inhibition enhances the anti-tumour activity of TMZ, confirming MGMT as a relevant pharmacological target. These data suggest that inhibition of MGMT should be further investigated especially in regimens containing TMZ and identification of new drug combinations for irinotecan are warranted for the treatment of high-risk neuroblastomas.
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Acknowledgements
The authors thank Tsen-Yin (Jackie) Lin and Michael A Sheard for their help with flow cytometry analysis, Martine Torres for her critical review of the paper. This work was supported in part by awards from the National Cancer Institute, CA081403 and CA82830, and by the Neil Bogart Memorial Laboratories of the TJ Martell Foundation for Leukemia, Cancer and AIDS Research.
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Cai, W., Maldonado, N., Cui, W. et al. Activity of irinotecan and temozolomide in the presence of O6-methylguanine-DNA methyltransferase inhibition in neuroblastoma pre-clinical models. Br J Cancer 103, 1369–1379 (2010). https://doi.org/10.1038/sj.bjc.6605927
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DOI: https://doi.org/10.1038/sj.bjc.6605927