Rab11-dependent recycling of calcium channels is mediated by auxiliary subunit α2δ-1 but not α2δ-3

Meyer, James O.; Dolphin, Annette C.

doi:10.1038/s41598-021-89820-1

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Published: 13 May 2021

Rab11-dependent recycling of calcium channels is mediated by auxiliary subunit α₂δ-1 but not α₂δ-3

Scientific Reports volume 11, Article number: 10256 (2021) Cite this article

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Abstract

N-type voltage-gated calcium channels (Ca_V2.2) are predominantly expressed at presynaptic terminals, and their function is regulated by auxiliary α₂δ and β subunits. All four mammalian α₂δ subunits enhance calcium currents through Ca_V1 and Ca_V2 channels, and this increase is attributed, in part, to increased Ca_V expression at the plasma membrane. In the present study we provide evidence that α₂δ-1, like α₂δ-2, is recycled to the plasma membrane through a Rab11a-dependent endosomal recycling pathway. Using a dominant-negative Rab11a mutant, Rab11a(S25N), we show that α₂δ-1 increases plasma membrane Ca_V2.2 expression by increasing the rate and extent of net forward Ca_V2.2 trafficking in a Rab11a-dependent manner. Dominant-negative Rab11a also reduces the ability of α₂δ-1 to increase Ca_V2.2 expression on the cell-surface of hippocampal neurites. In contrast, α₂δ-3 does not enhance rapid forward Ca_V2.2 trafficking, regardless of whether Rab11a(S25N) is present. In addition, whole-cell Ca_V2.2 currents are reduced by co-expression of Rab11a(S25N) in the presence of α₂δ-1, but not α₂δ-3. Taken together these data suggest that α₂δ subtypes participate in distinct trafficking pathways which in turn influence the localisation and function of Ca_V2.2.

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Introduction

The Ca_V1 and Ca_V2 voltage-gated calcium channels associate with auxiliary α₂δ and ß subunits¹. The α₂δ subunits are glycosyl-phosphatidylinositol-anchored extracellular proteins^2,3,4. There are four known mammalian α₂δ isoforms (α₂δ-1-4) which are thought to have similar functions and topological features, despite a surprising lack of sequence homology between them⁵. The α₂δ subunits have long been known to enhance whole-cell currents through Ca_V channels^6,7,8,9. However, to date there is limited evidence that α₂δs strongly influence single-channel properties of Ca_V channels⁸. As such, α₂δ-mediated enhancement of Ca_V currents has often been attributed primarily to increased channel trafficking and plasma membrane insertion^{8,10,11,12,13}.

The development of functional exofacially-tagged Ca_V2 constructs¹⁰, has allowed us to define populations of plasma membrane-inserted Ca_V2 channels using imaging approaches. This method was previously used to demonstrate that heterologous expression of α₂δ-1 increases cell-surface Ca_V2.2 expression, consistent with earlier models of α₂δ-1 function¹⁰. However, subsequent studies on the proteolytic processing of α₂δ have found that unprocessed α₂δ-1 is unable to enhance macroscopic Ca_V currents despite increasing cell-surface Ca_V2.2 expression in cell lines^14,15. These studies provide convincing evidence that the α₂δ-mediated increase in Ca_V currents is dependent on an increase in cell-surface Ca_V2.2 expression, but also requires a molecular activation switch, triggered by the proteolytic cleavage of α₂δ into α₂ and δ. Presently, it is unclear whether α₂δ isoforms differ significantly in their ability to enhance Ca_V currents, and it remains to be seen if α₂δ subtypes have uniform trafficking mechanisms particularly with regard to their effect on Ca_V localisation.

In a previous study¹⁶, we found that the cell-surface localization of α₂δ-2, following its heterologous expression, was increased by recycling from Rab11a-dependent endosomes back to the plasma membrane. Rab11a belongs to the Rab family of small GTPases which regulate a multitude of intracellular trafficking pathways, facilitating membrane targeting, cargo sorting and vesicle fusion events through recruitment of effectors and direct interactions with cargo proteins¹⁷. Rab11 proteins are particularly involved in trafficking through recycling endosomes^18,19, and inhibition of Rab11a-dependent recycling can be achieved through expression of the dominant-negative mutant Rab11a(S25N), which is locked in an inactive GDP-bound conformation²⁰. Cell-surface expression of α₂δ-2 is reduced in the presence of Rab11a(S25N)¹⁶, and this occludes the effect of the α₂δ ligand gabapentin, which itself reduces cell-surface expression of α₂δ-1 and α₂δ-2^11,16. These data provide a mechanistic explanation for the action of gabapentin, whereby α₂δ bound to gabapentin is prevented from recycling to the plasma membrane via Rab11-positive endosomes, resulting in a loss of α₂δ cell-surface expression¹⁶. Mutational studies have revealed that gabapentin acts by binding to a site including an RRR motif in the α₂ domain, which is present in α₂δ-1 and α₂δ-2^21,22,23,24. However, since α₂δ-3 is also widely distributed in the nervous system, if there were functional differences, for example between α₂δ-1 and α₂δ-3, this might influence the trafficking and localisation of associated Ca_V channels.

In the present study, we wished to determine to what extent the enhancement of plasma membrane Ca_V2.2 expression, a previously-demonstrated feature of α₂δ-1 function, is conserved for α₂δ-3. We then sought to elucidate further the mechanisms through which α₂δs increase cell-surface Ca_V2.2, and to understand how this process influences the localization and function of Ca_V2.2 in cell lines and hippocampal neurons. We find that α₂δ subunits have a differential effect on Ca_V2.2 trafficking; although all α₂δ proteins tested increase Ca_V2.2 cell-surface expression to varying extents, only α₂δ-1 and α₂δ-2 rapidly increase net forward trafficking of Ca_V2.2; by contrast α₂δ-3 does not influence this process, within a 45-min time-frame. Further examination showed that the enhanced forward trafficking of Ca_V2.2 is a Rab11a-dependent effect and that α₂δ-1 and α₂δ-2 participate in Rab11a-dependent recycling, whereas α₂δ-3 does not.

Results

Rab11a-dependent recycling affects α₂δ-1 but not α₂δ-3 cell-surface expression

Trafficking through Rab11a-positive recycling endosomes enhances α₂δ-2 membrane expression, a pathway that is inhibited by gabapentin¹⁶. The α₂ domain of both α₂δ-1 and α₂δ-2 contains a triple RRR motif, the third R of which is required for gabapentin binding, and is absent from the α₂δ-3 and α₂δ-4 sequences^12,25. Here, we considered the possibility that α₂δ-1 and α₂δ-2 enhance cell-surface Ca_V2.2 expression, at least in part, by facilitating the recycling of Ca_V2.2 through Rab11a-positive endosomes. Further to this, we speculated that α₂δ-3 may traffic independently of this pathway.

To test this hypothesis, we have used the dominant-negative Rab11a(S25N) mutant, which we found previously to inhibit cell-surface expression of α₂δ-2¹⁶, and thus a similar result was expected for α₂δ-1. In our previous study¹⁶ we found no effect of co-expressing WT Rab11a on any aspects of α₂δ trafficking or calcium channel function, and thus it was not included as an additional control in the present study. We compared cell-surface expression of HA-tagged α₂δ-1 and α₂δ-3, in the absence and presence of Rab11a(S25N) (Fig. 1A,B). Cell-surface α₂δ-3 immunostaining is typically weaker than that for α₂δ-1 (Fig. 1A), so we used antigen retrieval to maximise available HA signal. We found a reduction in cell-surface α₂δ-1-HA staining of 47%, when co-expressed with Rab11a(S25N) (Fig. 1B). In contrast α₂δ-3 showed no significant difference in cell-surface expression whether expressed with or without Rab11a(S25N) (Fig. 1B). These data provide evidence that α₂δ-1, like α₂δ-2¹⁶, is regulated by Rab11a-dependent recycling and suggest that cell-surface expression of α₂δ-3 is not promoted by trafficking through this endosomal recycling pathway (Fig. 1D). This result was supported by measurement of the intracellular α₂δ levels which were strongly reduced by Rab11a(S25N) in the case of α₂δ-1 but not α₂δ-3 (Fig. 1A,C). This suggests that block of recycling endosome function may lead re-routing of α₂δ-1 to degradation pathways (Fig. 1D), although this will require further experimentation.

Cell-surface Ca_V2.2 expression is reduced by Rab11a(S25N) when α₂δ-1 or α₂δ-2 are present

In our previous study¹⁶, we found that inhibition of α₂δ-2 recycling through Rab11a-positive endosomes reduced whole-cell Ca_V2.2 currents. However, a direct effect on plasma membrane insertion of associated Ca_V channels was not examined. Here we compared the plasma membrane expression of exofacially-tagged Ca_V2.2, containing a bungarotoxin binding site (BBS) tag¹⁰, when co-expressed with ß1b, either alone or together with α₂δ-1, or α₂δ-3. We then examined the effect of Rab11a(S25N) (Fig. 2A). Plasma membrane-inserted Ca_V2.2 was increased by 92% and 38% when co-expressed with α₂δ-1 and α₂δ-3, respectively (Fig. 2B), in agreement with our previous study¹⁴. However, Rab11a(S25N) co-expression reduced the plasma membrane level of Ca_V2.2 by 53% when α₂δ-1 was present, but had no effect in the presence of α₂δ-3, or in the absence of α₂δ (Fig. 2B). Total Ca_V2.2 levels were also reduced by Rab11a(S25N) in the presence of α₂δ-1 but not in the absence of α₂δ or presence of α₂δ-3 (Fig. 2A,C). Following these findings, we also compared the effect of Rab11a(S25N) on plasma membrane Ca_V2.2 in the presence α₂δ-2 (Fig. S1A,B). As expected, cell-surface Ca_V2.2 expressed with α₂δ-2 and β1b was reduced by 35% by Rab11a(S25N) (Fig. S1B).

Together, these data suggest that α₂δ-1 and α₂δ-2 enhance cell-surface Ca_V2.2 expression, in part through Rab11a-dependent recycling to the plasma membrane, which Ca_V2.2 is unable to access independently of these α₂δ subunits. In contrast, although α₂δ-3 produced a 38% increase in Ca_V2.2 cell-surface expression (Fig. 2B), this was independent of a Rab11a-dependent recycling pathway, suggesting that α₂δ-3 is only able to promote cell-surface Ca_V2.2 trafficking through another pathway, such as direct trafficking from the Golgi apparatus.

Rab11a(S25N) reduces Ca_V2.2 expression in neurites of cultured hippocampal neurons when co-expressed with α₂δ-1 but not α₂δ-3

We have previously demonstrated that Ca_Vß subunits are able to increase plasma membrane expression of Ca_V2.2 in non-neuronal cells, even in the absence of α₂δ, although there is generally an approximately twofold increase in the presence of α₂δ-1^10,14 (see Fig. 2B). However, in primary hippocampal neuronal cultures, we have found the presence of α₂δ to be critical for localisation of Ca_V2.2 to neurites¹⁴. Here, we examined whether Ca_V2.2 expression on the surface of hippocampal neurites is also influenced by Rab11a in an α₂δ-dependent manner. To do this we used an exofacially HA-tagged and N-terminally GFP-tagged Ca_V2.2 construct²⁶, which allowed us to quantify cell-surface and total Ca_V2.2 expression in non-permeabilised neurons. We expressed GFP-Ca_V2.2-HA plus β1b, with or without α₂δ-1 or α₂δ-3, and with or without Rab11a(S25N) (Fig. 3A). In accordance with our previous studies¹⁴, expression of Ca_V2.2 on the cell-surface of neurites (which had the appearance of dendrites, see Methods) was extremely low in the absence of α₂δ (Fig. 3A,B), and we found no effect of co-expression of Rab11a(S25N) on this. We observed a ~ fourfold increase in cell-surface Ca_V2.2-HA expression with α₂δ-1, and ~ 3.5-fold increase with α₂δ-3, relative to control (Fig. 3B). Expression of GFP in the neurites, representing total Ca_V2.2, was also elevated by co-expression of both α₂δ-1 and α₂δ-3, by 2.1-fold and 1.9-fold, respectively (Fig. 3C). Consistent with our findings in non-neuronal cells, cell-surface Ca_V2.2-HA was reduced by co-expression of Rab11a(S25N) in the presence of α₂δ-1 (by ~ 44%), but was not affected in the presence of α₂δ-3 (Fig. 3B). However, we saw no change in total Ca_V2.2-GFP expression in the neurites with Rab11a(S25N), either in the absence or presence of α₂δ-1 or α₂δ-3 (Fig. 3C). Together these data suggest that Rab11a-dependent recycling enhances neurite Ca_V2.2 cell-surface expression in an α₂δ-selective manner.

Rab11a(S25N) reduces whole-cell Ca_V2.2 currents when co-expressed with α₂δ-1 but not α₂δ-3

Here we investigated whether the reduction in cell-surface Ca_V2.2 expression by Rab11a(S25N) correlates to a change in whole-cell Ca_V currents and whether any change we observe is α₂δ-dependent. We used patch-clamp recording to measure whole-cell Ba²⁺ currents in tsA201 cells expressing Ca_V2.2/β1b/α₂δ, with or without Rab11a(S25N) (Fig. 4A). We found that the peak Ba²⁺ current density was reduced by 59% at + 10 mV, when α₂δ-1 and Rab11a(S25N) were co-expressed, compared to α₂δ-1 expressed alone (Fig. 4A,B). Corresponding maximum conductance (G_max) values were reduced from 1.4 ± 0.2 to 0.7 ± 0.1 nS/pF by the presence of Rab11a(S25N) (Fig. 4C). We saw no significant changes to steady-state inactivation under the two conditions (Fig. S2A), supporting the view that current density changes occurred as a result of reduced plasma membrane Ca_V2.2 expression rather than changes to biophysical properties of the channels.

Having established that Rab11a(S25N) reduced both α₂δ-1-mediated Ca_V2.2 plasma membrane expression and Ca_V2.2 current enhancement to similar extents, it was necessary to determine whether Rab11a(S25N) would produce a reduction in α₂δ-3-mediated Ca_V2.2 current enhancement. In contrast with the effect of α₂δ-1, we found no difference in mean current density (Fig. 4D,E), G_max (Fig. 4F) or steady-state inactivation (Fig. S2B) when Rab11a(S25N) was co-expressed with α₂δ-3, compared to α₂δ-3 alone. This result is consistent with our observation that cell-surface expression of Ca_V2.2 is unaffected by Rab11a-dependent recycling when expressed with α₂δ-3.

Net forward trafficking of Ca_V2.2 is enhanced by α₂δ-1 and α₂δ-2, but not α₂δ-3

Having provided evidence that α₂δ-3 enhances steady-state plasma membrane Ca_V2.2 expression, albeit to a smaller degree than α₂δ-1 (Fig. 2B), we considered two possible explanations for this effect: (1) α₂δ subunits increase forward trafficking of Ca_V2.2 to the cell-surface, or (2) α₂δ subunits reduce the rate of Ca_V2.2 endocytosis from the cell-surface. Previously, we found that the rate of Ca_V2.2 endocytosis was not altered by expression of α₂δ-1^10,27. In the present study, we used an α-bungarotoxin (BTX) live-labelling approach to compare the rates of net forward trafficking of Ca_V2.2-BBS expressed with β1b either alone or together with α₂δ-1, α₂δ-2 or α₂δ-3 (Fig. 5A). When compared to control conditions, we found that co-expression of α₂δ-1 and α₂δ-2 produced significantly higher insertion of Ca_V2.2 into the plasma membrane, at each time point tested, starting at 15 min (Fig. 5B). In contrast, the appearance of Ca_V2.2 on the cell-surface did not differ significantly between the control and α₂δ-3 conditions up to 45 min, but there was a statistically significant increase of 23% at 60 min (Fig. 5B), which is consistent with our observation of its effect on steady-state Ca_V2.2 expression (Fig. 2B). Estimates for initial rates of net forward Ca_V2.2 trafficking were made using the slope value of a straight line between 0 and 15 min. While there was a 2.7-fold increase in initial rate of Ca_V2.2 appearance on the cell-surface with α₂δ-1 co-expression compared to control (Fig. 5C), we found no significant difference in initial Ca_V2.2 trafficking rates for the α₂δ-3 condition. Together these data support a role for α₂δ-1 in enhancing rapid plasma membrane-insertion of Ca_V2.2 by increasing the rate of forward trafficking, whereas α₂δ-3 had no clear effect on the initial rate of forward Ca_V2.2 trafficking (Fig. 5C).

Dominant-negative Rab11a reduces net forward trafficking of α₂δ-1

Next, we compared net forward trafficking rates of α₂δ-1-BBS in the presence or absence of Rab11a(S25N) (Fig. 6A). We found that net forward trafficking of α₂δ-1-BBS was reduced by co-expression of Rab11a(S25N), with a 41% reduction in cell-surface α₂δ-1-BBS after 30 min (Fig. 6B), consistent with the reduction in cell-surface α₂δ-1-HA induced by Rab11a(S25N) under steady-state conditions (Fig. 1B). We then compared net forward trafficking of Ca_V2.2-BBS, when expressed with or without α₂δ-1 and with or without Rab11a(S25N), at two time points, 10 and 30 min (Fig. 6C–E). As expected, α₂δ-1 enhanced net-forward trafficking of Ca_V2.2, and Rab11a(S25N) abolished this increase at both time points. In sum, these data support the conclusion that α₂δ-1 membrane expression is enhanced by forward-trafficking from Rab11a-positive recycling endosomes, and that Ca_V2.2 can traffic through this pathway when co-expressed with Rab11a-sensitive α₂δ-1, but not with α₂δ-3 (Fig. 1D).

Discussion

In this study we have shown that α₂δ subunits differentially enhance plasma membrane expression of Ca_V2.2 in both cell lines and primary neuronal cultures, and have implicated Rab11a-dependent recycling in this process. Rab11 proteins are particularly involved in trafficking through recycling endosomes^18,19, and here we inhibited this route through expression of the dominant-negative mutant Rab11a(S25N)²⁰. Our data indicate that α₂δ-1 and α₂δ-2 promote both the steady-state cell-surface Ca_V2.2 level and net forward trafficking of Ca_V2.2 through a Rab11a-dependent mechanism (Fig. 1D). Blockade of this pathway leads to a reduction in cell-surface Ca_V2.2 and α₂δ-1 and may lead to increase in degradation, as judged by the reduction in their intracellular levels. However, α₂δ-3 does not appear to participate in the Rab11a-dependent recycling pathway, and does not increase net forward Ca_V2.2 trafficking in the short-term (Fig. 5B,C). Despite this, α₂δ-3 consistently increases plasma membrane-inserted Ca_V2.2 under steady-state conditions¹⁴; and the present study, although to a smaller extent than α₂δ-1. Inhibition of Rab11a-dependent recycling also reduces whole-cell Ca_V2.2 currents, when expressed with α₂δ-1, but has no effect with α₂δ-3. Our results indicate that one of the main drivers of α₂δ-1-mediated enhancement of Ca_V2.2 plasma membrane-insertion is recycling back to the plasma membrane via recycling endosomes. In primary hippocampal neurites, we find that Ca_V2.2 membrane expression is largely dependent on α₂δ, and inhibition of Rab11a-dependent recycling also lowers cell-surface Ca_V2.2 in neurites in the presence of α₂δ-1. The neurites examined here were of dendritic appearance, 100–150 μm from the soma, and in future it will be of interest to also examine trafficking in axons, because of the differential distribution of α₂δ subunits in neurons^28,29.

Earlier work from this laboratory has shown that α₂δ-2 participates in recycling through Rab11a-positive recycling endosomes and that this pathway is interrupted by gabapentin¹⁶. The gabapentin-binding RRR motif is positioned just upstream of the VWA domain in both α₂δ-1 and α₂δ-2, and it has been hypothesised that gabapentinoids displace an endogenous ligand in the binding pocket that includes the RRR motif^11,21,30. Previous studies using the α₂δ-1(R217A) or α₂δ-2(R282A) mutants, which show much lower affinity for binding gabapentin, found them to have lower ability to enhance Ca_V2 currents compared to their wild-type counterparts, as well as reduced endosomal recycling^11,16,21. These findings are consistent with the hypothesis that binding of an endogenous ligand in the gabapentin binding site is important for association of α₂δ-1 and α₂δ-2 with endosomal sorting partners. As α₂δ-3 lacks a gabapentin binding site, we hypothesised that it would not participate in Rab11a-dependent recycling and would thereby be unable to promote forward trafficking of Ca_V2.2 through this pathway, although the direct route via the Golgi apparatus would still be available¹⁶. In agreement with this, we find inhibition of Rab11a-dependent recycling did not influence α₂δ-3 trafficking, unlike for α₂δ-1 or α₂δ-2. Presently it is unclear why α₂δ-3 does not participate in Rab11a-dependent recycling, although α₂δ-3 shares only 25.7% sequence homology with α₂δ-1 (ClustalO), which may result in conformational differences that preclude an interaction with a Rab11a effector.

Since Rab11a is a membrane-associated cytoplasmic protein, and α₂δ-1 is extracellular and therefore present in the lumen of recycling endosomes, any functional interaction between the two will involve other proteins. There are several studies that have examined the interaction of α₂δs with other proteins; for review see³¹. For example, we have demonstrated an interaction between α₂δ-1 and Low-Density Lipoprotein (LDL) Receptor-related Protein-1 (LRP1), a multifunctional receptor which mediates cargo trafficking. LRP1 and its chaperone Rap, promote α₂δ-1 maturation and trafficking to the cell-surface, and also enhance Ca_V2.2 currents³². Since LRP1 has multiple intracellular and extracellular protein ligands, and contains several cytoplasmic adaptor protein binding motifs³³, it may play a role in α₂δ-1 trafficking via recycling endosomes.

In another study, neurexin-1α enhanced Ca_V2.1 currents when expressed with α₂δ-1 but had no effect in the presence of α₂δ-3³⁴, although no selective and specific interaction with neurexin-1α could be demonstrated, in contrast to a previous study³⁵. α₂δ-1 has also been reported to interact with thrombospondins (TSPs) 1–4, which are extracellular matrix proteins³⁶. More recently, another study³⁷ showed a weak interaction between α₂δ-1 and TSP4, but was unable to demonstrate any interaction between cell-surface expressed α₂δ-1 and TSP4, concluding that α₂δ-1 and TSP4 may only interact intracellularly at high concentrations. A further study showed a low affinity interaction between α₂δ-1 and TSP4, but not other TSPs³⁸. However, any interaction of α₂δ subunits with TSPs is unlikely to be involved in their Rab11-dependent recycling.

A number of studies have also linked Rab11a function to growth cone development^39,40,41. Since we have demonstrated distinct trafficking pathways for α₂δ-1 and α₂δ-2 compared to α₂δ-3 in relation to Rab11a-dependent recycling, it is possible that other demonstrated subtype-specific functions of α₂δ proteins^28,42 may also involve distinct trafficking pathways.

Materials and methods

Molecular biology

The following cDNAs were used: Ca_V2.2 (rabbit, D14157), Ca_V2.2-HA and Ca_V2.2-BBS¹⁰, GFP-Ca_V2.2-HA²⁶, β1b (rat, X61394)⁴³, α₂δ-1 (rat, M86621)⁴⁴, α₂δ-1-HA^12,45, α₂δ-1-BBS, in which the BBS tag from α₂δ-2¹⁶ was inserted into α₂δ-1 in the same position as the HA tag, α₂δ-3 (AJ010949), α₂δ-3-HA¹⁴. mCherry⁴⁶ or CD8⁴⁷ were included as a transfection markers where stated. Human Rab11a (AF000231) containing the S25N mutation^16,48, was obtained from From Prof. T. Hébert, McGill University.

Antibodies and other materials

Antibodies (Abs) used were: anti-HA Ab (rat monoclonal, Roche), anti-Ca_V2.2 II-III loop Ab (rabbit polyclonal)⁴⁹. The following secondary Abs (raised in goat) were used: anti-rat or anti-rabbit IgG-Alexa Fluor (AF) 488 or 594 (ThermoFisher), biotin-labelled anti-rat IgG (Sigma). Other reagents used were BTX-AF488 (Invitrogen) and streptavidin-AF633 (Invitrogen).

Cell lines: culture and transfection

tsA-201 cells were grown in Dulbecco's Modified Eagle Medium (DMEM) (Invitrogen) supplemented with 10% Foetal Bovine Serum, 50 U/ml penicillin/streptomycin and 1% GlutaMAX (Invitrogen). Cells were maintained in tissue culture flasks at 37 °C in a humidified atmosphere of 5% CO₂ and grown up to 70–90% confluence prior to transfection or further passage. Neuro2a (N2a) cells were cultured in 50% DMEM (with high glucose and L-glutamine) and 50% Opti-MEM (with L-glutamine), supplemented with 50 U/ml penicillin/streptomycin, 5% FBS, and 1% GlutaMAX (Life Technologies). The N2a cells were cultured to 80% confluence in a 5% CO₂ incubator at 37 °C and passaged every 3 to 4 d.

For transient expression, N2a or tsA-201 cells were transfected using either PolyJet (SignaGen Laboratories) or Fugene 6 (Promega) in a 3:1 ratio with cDNA mix, according to the manufacturers’ instructions. A typical cDNA mix was 2 μg total DNA per 35 mm culture dish at a molar ratio of 3:2:2 of Ca_V2.2: β1b: α₂δ. In experiments using Rab11a(S25N) the cDNA mix was 3:2:2:1 for Ca_V2.2: β1b: α₂δ: Rab11a(S25N). In conditions lacking a given construct, the DNA mix was supplemented with the appropriate empty vector to have the same total DNA. For fluorescence imaging experiments in either tsA-201 or N2a cells, all cDNAs were in a pcDNA3, pRK5 or pCMV vector. In electrophysiological experiments using tsA-201 cells, all cDNAs were in a pMT2 vector, with the exception of Rab11a(S25N) (pCMV).

Hippocampal neuronal culture and transfection

Hippocampal neurons were isolated from the hippocampus of P0 rats, which were killed under a UK Home Office Schedule 1 procedure. The cerebrum was cut into two, and the hippocampi were extracted from each hemisphere in ice cold HBSS with 10 mM HEPES. The hippocampi were then cut into small segments and digested gently in Papain solution (7 U/ml Papain, 0.2 mg/ml L-Cysteine, 0.2 mg/ml Bovine Serum Albumin (BSA), 5 mg/ml glucose, 10 mM HEPES in HBSS with 30 Kunitz/ml DNase) in a shaking water bath for 40 min at 37 °C. The cells were washed twice with the growth medium (Neurobasal, 2% B27 supplement, 1 unit/ml penicillin, 1 μg/ml streptomycin, 1% GlutaMAX, 0.1% β-mercaptoethanol), and triturated gently. The cells were plated onto 18 mm circular coverslips that are coated with poly-L-lysine and laminin at 750 cells/μl, 100 μl per coverslip. The entire growth medium was changed after 2 h of plating, and half of the medium was then changed every 3–4 d.

Hippocampal neurons were transfected after 7 d in culture, using cDNAs cloned into pCAGGS⁵⁰. 2 h prior to transfection, the growth medium was replaced with a mixture of 50% conditioned, and 50% fresh growth medium. The transfection process was as follows: 4 μg cDNA was mixed in a 50 μl total with Opti-MEM (Thermo Fisher), 2 μl Lipofectamine 2000 (Thermo Fisher) was mixed in a 50 μl total volume of Opti-MEM in separate tubes by pipetting; mixing was done by gentle pipetting. DNA and transfection reagent mixes were then combined and incubated for 5 min at room temperature before being added dropwise to cultures. Hippocampal neurons were cultured for a further 7 d after transfection before fixation and immunostaining.

Immunocytochemistry

Cells were plated onto either 20 mm square coverslips, or glass-bottomed dishes (MatTek Corporation) which were coated with poly-L-lysine prior to transfection, and cultured in a 5% CO₂ incubator at 37 °C. After 36–48 h expression, cells were fixed with ice cold 4% paraformaldehyde (PFA), 4% sucrose in PBS, pH 7.4 at room temperature for 5 min. Where antigen retrieval was used, fixed samples were incubated in pH 6 citrate buffer at 98˚C for 10 min¹⁰. For labelling the HA epitope on the cell-surface in non-permeabilised conditions, cells were incubated with primary Ab with 2% BSA and 10% goat serum in PBS at room temperature for 1 h for cell lines or overnight at 4 °C for neurons (Fig. 3). Using the same protocol, no detectable permeabilization by PFA fixation in hippocampal neurons was observed in a previous study⁵¹. When permeabilization was required, cells were incubated with 0.2% Triton X-100 in PBS for 5 min¹⁰. The secondary Ab was added with 2.5% BSA and 10% goat serum in PBS and incubated for 1 h at room temperature. Cell nuclei were stained with 0.5 µM 4′,6′-diamidino-2-phenylindole (DAPI) in PBS for 10 min. The coverslips were mounted onto glass slides using Vectashield mounting medium (Vector Laboratories).

In experiments probing for both cell-surface and intracellular HA epitopes (Fig. 1), cells were fixed and blocked as described above, then immunostained for 1 h with rat anti-HA Ab (1:500), followed by 1 h incubation with biotin-labelled anti-rat IgG (1:1000). Following this, cells were permeabilised with 0.2% Triton-X100 for 5 min and re-probed with rat anti-HA Ab for 1 h. Cells were then incubated with anti-rat-AF594 Ab (1:500) and streptavidin-AF633 (1:500), to visualise intracellular and extracellular HA epitopes, respectively. In experiments probing for cell surface BBS tag in Ca_V2.2 (Fig. 2, Fig. S1), cells were live-labelled with BTX-AF488 (10 µg/ml, Invitrogen) for 30 min at 17 °C. Cells were then washed, fixed, permeabilised and immunostained, using a rabbit Ab against the II-III loop to detect total Ca_V2.2, followed by secondary anti-rabbit-AF594 Ab.

Net forward trafficking assay

Transfected cells were plated onto 22 × 22 mm glass coverslips coated with poly-L-lysine, and cultured in a 5% CO₂ incubator at 37 °C. N2a cells were used for Ca_V2.2-HA experiments and tsA-201 cells for α₂δ-1 experiments, where higher expression levels were required to observe live labelling. After 40 h expression, cells were washed twice with Krebs–Ringer-HEPES (KRH) buffer and incubated with 10 μg/ml unlabelled BTX (Life technologies) for 30 min at 17 °C. The unbound BTX was washed off with KRH, and the cells were then incubated with BTX-AF488 (10 µg/ml) in KRH at 37 °C. To terminate the reaction, cells were washed twice with cold KRH and then fixed with 4% PFA, 4% sucrose in PBS at specified times for the kinetic assay. After fixation, cells were permeabilised and intracellular expression markers and/or nuclei were labelled as described above. The coverslips were then mounted onto glass slides using Vectashield mounting medium.

Confocal microscopy and analysis

All images were acquired using an LSM 780 scanning confocal microscope (Zeiss), equipped with a Plan-Apochromat 63x/1.4 or 40x/1.3 DICII oil immersion objective lens, in 16-bit mode. For experiments in which both AF594 and AF633 were used, sequential scanning was performed with 633 nm and 594 nm laser lines, with emission filters 638–747 nm or 597–695 nm, with beam splitters (MBS) 488/543/633 and MBS 458/514/594, respectively. For each experiment, the laser power, gain and acquisition settings were kept constant between images that were used for quantification, although laser power and gain settings were adjusted between experiments depending on expression and staining level of the samples. Where possible, the region of interest was determined by identifying cells with expression of a transfection marker or intracellular staining of the protein of interest (e.g. GFP, HA), without selecting for the cell-surface immunostaining, to avoid bias. In experiments where an appropriate intracellular expression marker was absent, nuclear staining with DAPI was used to identify viable cells (having an intact nucleus); cell-surface measurements were made for all viable cells per field of view. In addition, a 2 × 2 or 3 × 3 tile scan was performed to further remove the bias in selecting cells with high expression. For cell-surface expression analysis, images were taken usually with 1 μm optical section. Confocal images were imported and analysed in ImageJ (National Institutes of Health). The plasma membrane fluorescence was quantified using the freehand brush tool with a selection width of 0.66 μm and tracing the membrane region manually. If any cells show signs of intracellular staining for the extracellular epitope, because of damage-induced permeabilization, they are excluded from analysis. Total or intracellular fluorescence was quantified using the freehand selection tool, omitting the signal intensity from the nuclei. The background fluorescence in each channel was measured and subtracted from mean cell-surface or intracellular fluorescence measurements in image analysis.

For analysis of neurite expression in hippocampal neurons, an average of 10–15 cells were selected per condition for an individual experiment. Neurons were selected based on expression of free mCherry as a transfection marker. Between 2 and 5 neurites (with the appearance of dendrites) were measured per mCherry-positive cell to reduce over-representation of highly branched neurons. The freehand brush tool was used to manually trace neurites of lengths between 25 and 60 µm, and width of 2 µm, beginning at a distance of 100 µm from the cell soma. Neurites were selected and traced using mCherry to avoid bias, measurements were then taken in channels for expression of HA and/or GFP tags on expressed Ca_V2.2 constructs. Background fluorescence measurements were taken for each condition and subtracted from mean fluorescence values during image analysis. Mean neurite signal intensity for each channel was calculated for neurons of a given condition and normalized to the stated controls. Normalized data were then pooled between experiments with a minimum of three separate experiments.

In cell-surface expression and forward trafficking experiments, where relevant, all cells chosen for analysis contained intracellular Ca_V2.2 expression. For the forward trafficking assays, the membrane fluorescent intensities were fitted to the single exponential association. All experiments were repeated n = 3–5 times, and approximately 30–50 cells were analysed for each experiment. All data are presented as pooled cells, except for the trafficking initial rates, which are averages of separate experiments.

Electrophysiology

tsA-201 cells were transfected with cDNA mix containing Ca_V2.2, α₂δ-1, β1b, and CD8 at a ratio of 3:2:2:0.8 using a Fugene transfection protocol. After 40 h expression, cells were replated in cell culture medium at 1 in 3 or 1 in 5 dilution depending on their confluency. Transfected cells were identified by CD8 expression, detected with CD8 Dynabeads (Life Technologies). Whole-cell currents were recorded in voltage-clamp mode in the following solutions; intracellular solution (mM): 140 Cs-aspartate, 5 EGTA, 2 MgCl₂, 0.1 CaCl₂, 2 K₂ATP, 20 HEPES, pH 7.2, 310 mOsm; extracellular solution (mM): 1 BaCl₂, 3 KCl, 1 NaHCO₃, 1 MgCl₂, 10 HEPES, 4 D-glucose, 160 tetraethylammonium bromide, pH 7.4, 320 mOsm. The borosilicate glass electrode resistance was between 1.5 and 4 MΩ. Cell capacitance and series resistance were compensated to 60–70%. Whole-cell currents were recorded on Axopatch-200B amplifier using pClamp 9 or 10 (Molecular Devices). The cells were held at − 90 mV, and 50 ms pulses were applied in + 10 mV steps between − 50 mV and + 50 mV. To correct for the leak current, P/8 leak subtraction protocol was applied. Recordings were made at 20 kHz sampling frequency and filtered at 5 kHz (lowpass 4-pole Bessel filter) in the amplifier. The digital low-pass 8-pole Bessel filter with 1 kHz 3 dB cut-off was applied in Clampfit 10.7 (Molecular Devices) before the current amplitudes were determined. Average peak currents were taken between 8 and 13 ms after the test potentials were applied and normalized to the cell capacitance to obtain current density. IV relationships were fit by a modified Boltzmann equation as follows: I = G_max*(V-V_rev)/(1 + exp(-(V-V_{50, act})/k)), where I is the current density (in pA/pF), G_max is the maximum conductance (in nS/pF), V_rev is the apparent reversal potential (mV), V_{50, act} is the midpoint voltage for current activation (mV), and k is the slope factor.

Statistical analysis

The number of samples (n) in most of the experiments in this study is the total number of individual cells analysed, which are pooled from a minimum of three separate experiments unless stated otherwise. Data from individual experiments were normalized to their control conditions prior to being pooled to account for inter-experimental variance. Mean values and standard error of mean (SEM) were calculated for normalized, pooled data. The only exception is for the experiments determining the rates of Ca_V2.2 trafficking, where the mean and SEM values were determined from the mean of repeated experiments. Statistical analysis was performed using Student’s t test or one-way ANOVA with appropriate post-hoc test as stated, in GraphPad Prism 7.

Ethical approval

The use of neonatal mice for primary hippocampal cultures was carried out according to methods covered by UK Home Office Schedule I procedures. Ethical approval for Schedule I licence 1285 was obtained from the local UCL Animal Welfare Ethical Review Body (AWERB) and followed ARRIVE guidelines and regulations.

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Acknowledgements

The authors thank Wendy S Pratt and Kanchan Chaggar for technical support, and Dr. Laurent Ferron and Kjara Pilch for hippocampal cultures. We thank Drs. Karen Page and Manuela Nieto-Rostro for commenting on the manuscript.

Funding

This research was funded, in part, by the Wellcome Investigator award to ACD (098360/Z/12/Z)]. For the purpose of Open Access, the corresponding author has applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission. JOM was supported in part by the UCL Grand Challenges PhD program.

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Department of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, London, WC1E 6BT, UK
James O. Meyer & Annette C. Dolphin

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James O. Meyer
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J.O.M. performed all experimental work, and analysed data. A.C.D. analysed data. J.O.M. and A.C.D. conceived the study, co-wrote the manuscript and prepared the Figures.

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Correspondence to Annette C. Dolphin.

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Meyer, J.O., Dolphin, A.C. Rab11-dependent recycling of calcium channels is mediated by auxiliary subunit α₂δ-1 but not α₂δ-3. Sci Rep 11, 10256 (2021). https://doi.org/10.1038/s41598-021-89820-1

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Received: 01 March 2021
Accepted: 16 April 2021
Published: 13 May 2021
DOI: https://doi.org/10.1038/s41598-021-89820-1

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