Correction to: Scientific Reports https://doi.org/10.1038/s41598-021-00407-2, published online 28 October 2021
In the original version of this Article a previous rendition of Figure 2 was published.
The original Figure 2 and accompanying legend appear below.
The fluorescence of the induced fluorescent pseudopupil originates from the rhabdomeres. (a) Diagram of a single ommatidium of a dipteran compound eye. Dotted lines denote the plane where optical cross sections of the eye in (b–d) were taken. From top to bottom: corneal facet (C), pseudocone (PC) and distal tip of the rhabdomeres (Rh). (b,c) Eristalis tenax compound eye after application of Lucifer Yellow and scanned with a confocal microscope, with a 63× glycerol objective. Scale bar 100 µm. The fluorescence observed at the surface of the eye (b) originates from the fluorescent rhabdomere tips as no other cells or parts of the photoreceptors are fluorescent when we focus below the cornea to the plane of the rhabdom tips (c). (d) Magnified view from a single ommatidium, showing the distinctive trapezoidal shape formed by the distal tips of 7 adjacent rhabdomeres, typical of dipteran flies. Scale bar 5 µm. (e) Maximum intensity projection of a z-stack of the left eye of a female Eristalis tenax. When the dye (in this case Neurobiotin 488) had been left in the head for more than 3 h we experienced glowing in the entire eye. Image acquired with a Leica SP8 DLS confocal microscope and a 2.5× air objective lens (see also Supplementary Information video 1, part 2).
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Rigosi, E., Warrant, E.J. & O’Carroll, D.C. Publisher Correction: A new, fluorescence-based method for visualizing the pseudopupil and assessing optical acuity in the dark compound eyes of honeybees and other insects. Sci Rep 11, 22227 (2021). https://doi.org/10.1038/s41598-021-01737-x
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DOI: https://doi.org/10.1038/s41598-021-01737-x
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