Abstract
The gravity is necessary for living organisms to operate various biological events including hippocampus-related functions of learning and memory. Until now, it remains inconclusive how altered gravity is associated with hippocampal functions. It is mainly due to the difficulties in generating an animal model experiencing altered gravity. Here, we demonstrate the effects of hypergravity on hippocampus-related functions using an animal behavior and electrophysiology with our hypergravity animal model. The hypergravity (4G, 4 weeks) group showed impaired synaptic efficacy and long-term potentiation in CA1 neurons of the hippocampus along with the poor performance of a novel object recognition task. Our studies suggest that altered gravity affects hippocampus-related cognitive functions, presumably through structural and functional adaptation to various conditions of gravity shift.
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Introduction
Gravity shift renders living organisms to change their physiological properties and become evolved and adapted for stabilization to the current environment. Gravitational change such as microgravity (MG) and hypergravity (HG) is the most influential stressor to terrestrial and aquatic creatures from Animalia to Plantae1,2,3,4,5,6,7. As for human, astronauts who experience both MG (in outer space) and HG (in launch and re-entry)8 suffer from various adverse symptoms9,10,11. Gravity shift from the space to Earth can be considered much larger HG to astronauts, and they are likely to spend a long time under intensive HG. Also, the level of HG used for the study on enhancement of drug effect is massive12,13,14,15. Under this condition, the central nervous system (CNS) is susceptible to gravity in the context of movement control, sensory integration, locomotion, balance, circadian rhythm, and hormone release16,17,18,19,20,21,22,23,24,25,26,27,28,29.
Hippocampus is the essential part of CNS in light of learning and memory30,31,32,33,34,35,36,37,38. The effect of gravity on the hippocampus is investigated in various methods of behavioral, molecular, and electrophysiological aspects. MG causes the impaired discrimination of a new spatial arrangement39,40 and alteration in neural proteome39,41, gene expression42 and cytomorphometry43. Interestingly, short-term exposure of HG (4G, 48 h) results in enhanced LTP on CA144 while long-term exposure of HG (4G, 3 weeks) impairs spatial learning performance45. Various effects of gravity on hippocampus-related functions from genetic to molecular, behavioral, and electrophysiological levels are summarized in Table 139,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55, based on the duration and induction method of MG and HG. However, synaptic mechanism and behavioral consequence of HG on hippocampal functions still remain undetermined.
The aim of current study is to reveal a certain relationship between gravity and the hippocampal synaptic mechanisms underlying a cognitive function. For this, we employed a centrifugal system for gravity conditioning and electrophysiology (EPG) for observing synaptic responses of hippocampal CA1 neurons along with the behavior test of novel object recognition (NOR). Our results show that the ability to discriminate a novel object from the familiar one is impaired in the long-term exposure of HG (4 weeks) but not in the short-term exposure of HG (1 day). Furthermore, postsynaptic responses were reduced under long-term HG, largely due to the impairment of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs) and N-methyl-d-aspartate receptors (NMDARs). Here, we demonstrate that the long-term HG impairs hippocampus-related synaptic functions, therein suggesting the cellular mechanism of a HG-induced cognitive deficit and a therapeutic strategy.
Experimental procedures
Animals
About 11 weeks old male Sprague–Dawley (SD) rats were used for electrophysiology and animal behavior test. All animal handling procedures were approved by the Institutional Animal Care and Use Committee of Inha University (INHA 180105-533) and Incheon National University (INU-ANIM-2017-08), and all experiments were performed in accordance with relevant guidelines and regulations.
Hypergravity exposure
SD rats were conditioned by HG (4G) induced in a gravitational force simulator with two horizontal rotatory arms (50 cm long each). When the arms were rotated, centrifugal force was delivered to the animal cage locating at the end of arms. When the arms rotated at a speed of 65 rpm, rats in the cage were exposed to 4G. A high-resolution camera inside the cage was used to observe whether rats were able to move freely and access food and water. The rats were exposed to HG for 23 h and took an hour rest under normal gravity (1G). The conditioning process was repeated for 1 day (HG1day) or 4 weeks (HG4weeks) (Fig. 1a). The behavior test and EPG were conducted in 24 h right after the rats were released from HG (Fig. 1b).
Behavior test of a novel object recognition
NOR test comprised two phases. Each phase with 10 min duration was separated by a 6-h inter-phase interval. During the first phase of the familiarization period, the rat was presented in a pair of identical, familiar objects (F1, F2) in a white acrylic open field box (60 cm width × 60 cm length × 60 cm height) to be habituated to environmental factors including the place of objects. The two objects were placed in a diagonal position about 5 cm away from the white wall (Fig. 2a). The exploratory movement of the rat during the phase was recorded with a video camera installed at the top of the apparatus. In completing the first phase, the rat and familiar objects (F1, F2) were removed from the apparatus for 6 h. In the second phase, the test phase, the rat explored a third copy of the familiar object (F3) and a novel object (N) in the apparatus. Rats used to explore the novel object (N) more than the familiar one (F3). To eliminate a certain variation by emotional instability, rats with excessive freezing behavior more than 60% (> 360 s) out of the whole period of test phase (10 mins) were excluded from analysis (Exclusion : 1G—3 out of 10 rats; HG1day, HG4weeks—each 1 out of 5 rats). Object exploration was defined when the nose of the rats directed towards the object at a distance below 2 cm and measured by the discrimination index which indicates the difference of time spent between a novel (TN) and familiar object (TF3). It was calculated with the total amount of time spent with both objects in the test phase [Discrimination Index = (TN—TF3)/(TN + TF3)].
Brain slice preparation
Age matched conditioned (HG group) and unconditioned (1G group) rats were deeply anesthetized with 2% isoflurane. Motionless rats were decapitated by a guillotine, and the brain was isolated quickly. The isolated brain was transferred to the ice-cold dissection buffer containing the following ingredients (in mM): 25 glucose, 75 sucrose, 87 NaCl, 2.5 KCl, 1.3 NaH2PO4, 25 NaHCO3, 7.0 MgCl2, and 0.5 CaCl2 bubbled with a mixture of 5% CO2 and 95% O2. The hippocampus including Schaffer collaterals (SC) was isolated from the whole brain and transversely sectioned into 400 μm thick slices using Leica VT1200S vibratome (Leica Biosystems, Wetzlar, Germany) filled with the same buffer. The slices were incubated for 12 min at 32 °C and recovered for 1 h at room temperature, submerged in the artificial cerebral spinal fluid (aCSF) containing the following ingredients (in mM): 25 glucose, 125 NaCl, 2.5 KCl, 1.3 NaH2PO4, 25 NaHCO3, 1.0 MgCl2, and 2.0 CaCl2 bubbled with a mixture of 5% CO2 and 95% O2.
In vitro field recording
Slices were transferred to a recording chamber where aCSF flowed (31 ± 0.5 °C; 1–2 ml/min). A slice harp anchored the slices in order to stabilize the recording position. A bipolar stimulating electrode was placed on the SC to evoke field excitatory postsynaptic potential (fEPSP) which were recorded on the stratum radiatum (SR) of CA1 by glass electrodes filled with aCSF. All responses were acquired using Axon Digidata 1550B 8-Channel Digitizer (Molecular Devices, San Jose, CA) and amplified using MultiClamp 700B Microelectrode amplifier (Molecular Devices, San Jose, CA, USA). The maximum slope of fEPSPs was identified in the establishment of input–output (I/O) relationship. Half maximal fEPSPs were used for high frequency stimulation (HFS: 100 Hz, 1 s) for LTP induction.
Data analysis
All electrophysiological data were presented numerically using Axon pCLAMP11 Electrophysiology Data Acquisition and Analysis Software (Molecular Devices, San Jose, CA). The difference in behavior assessment was measured by One-way analysis of variance (ANOVA) of the Bonferroni post-hoc test. Two-way ANOVA was used to assess statistical significance for the differences between HG and 1G groups in EPG. Every statistical process was performed on SPSS Statics 25 (IBM, Armonk, NY). At least p < 0.05 was interpreted statistically significant (*p < 0.05, **p < 0.01, ***p < 0.001). All Graphs were prepared by GraphPad Prism 7 (GraphPad Software Inc., La Jolla, CA, USA) and final arrangement and labeling were carried out using Adobe Illustrator CC 2019 (Adobe Inc., San Jose, CA, USA). All data are presented in mean ± standard error of the mean (SEM). N indicates the number of animals in the NOR test and slices in EPG.
Result
Impaired NOR in rats under HG
We wondered whether the gravity affects memory-related behavior according to an exposure time of HG. The NOR task was conducted with rats under normal gravity, HG1day, and HG4weeks in open field box (Fig. 2a,b). Rats showed similar preference for each object regardless of HG in the familiarization phase (Fig. 2c; 1G—F1: 31.50 ± 5.64, F2: 37.58 ± 9.40, p = 0.590, HG1day—F1: 31.32 ± 4.79, F2: 30.40 ± 5.73, p = 0.906, HG4weeks—F1: 59.28 ± 10.82, F2: 52.37 ± 10.46, p = 0.662). 1G and HG1day had a preference for novel objects in the test phase but not HG4weeks rats (Fig. 2d; 1G—F3: 27.20 ± 3.12, N: 51.28 ± 4.06, p < 0.001, HG1day—F3: 22.46 ± 1.80, N: 47.38 ± 10.14, p = 0.052, HG4weeks—F3: 45.19 ± 7.56, N: 43.09 ± 5.59, p = 0.831). Discriminating competence differed between groups (Fig. 2e; 1G—0.31 ± 0.03, n = 7 rats, HG1day—0.33 ± 0.06, n = 4 rats, HG4weeks—0.02 ± 0.04, n = 4 rats, F2,12 = 18.224, p < 0.001). The 1G and HG1day groups preferred a novel object whereas the HG4weeks group showed the similar preference toward both objects (posttest: 1G vs. HG1day—p = 1.000, 1G vs. HG4weeks—p < 0.001, HG1day vs. HG4weeks—p = 0.001). To determine if HG influences motivation and locomotion for rats to explore, total exploration time was measured (Fig. 2f; 1G—78.47 ± 6.91, n = 7 rats, HG1day—69.84 ± 11.74, n = 4 rats, HG4weeks—88.28 ± 12.78, n = 4 rats, F2,12 = 0.727, p = 0.503), showing no significant difference between all groups. It indicates that HG4weeks impairs the ability to discriminate a novel object from the familiar one.
Reduced postsynaptic, but not presynaptic transmission under HG
In order to examine whether HG affects synaptic events in the hippocampal CA1 network, we tested the synaptic transmission of CA1 pyramidal cells in response to SC stimulation. The fiber volley (FV), the indicator of Ca2+ influx into the presynaptic axon terminal, was considered as the input while the slope of postsynaptic fEPSPs, mostly AMPARs-mediated responses, was taken as the output. As predicted, the slope of fEPSPs increased as the amplitude of FVs increased in both groups (Fig. 3a,b; 1G—0.2 mV: 0.15 ± 0.02, n = 27 slices, 0.4 mV: 0.38 ± 0.03, n = 30 slices, 0.6 mV: 0.66 ± 0.14, n = 14 slices; HG—0.2 mV: 0.08 ± 0.01, n = 30 slices, 0.4 mV: 0.23 ± 0.06, n = 22 slices, 0.6 mV: 0.34 ± 0.11, n = 10 slices). However, the significant deficit of synaptic transmission was observed in the HG group when it is compared to that in the 1G group (F1,127 = 14.747, p < 0.001). A post-hoc Student’s t tests showed the significant difference in output at 0.2 and 0.4 mV (t0.2 mV = 3.091, p0.2 mV = 0.004, t0.4 mV = 2.217, p0.4 mV = 0.033, t0.6 mV = 1.766, p0.6 mV = 0.091). Next, to test whether the response difference is attributed to altered function of NMDARs, NMDAR response as the function of FVs was measured in the presence of 10 μM NBQX, an AMPAR antagonist, and 0 mM MgCl2, an NMDAR-enhancing chemical. We found that the HG group had the significant reduction in NMDAR response when compared with that of the 1G group (Fig. 3c; 1G—0.2 mV: 0.32 ± 0.04, n = 10 slices, 0.4 mV: 0.60 ± 0.10, n = 12 slices, 0.6 mV: 1.19 ± 0.04, n = 4 slices; HG—0.2 mV: 0.18 ± 0.02, n = 5 slices, 0.4 mV: 0.35 ± 0.05, n = 10 slices, 0.6 mV: 0.57 ± 0.10, n = 11 slices; F1,46 = 32.989, p < 0.001). A post-hoc Student’s t tests showed the significant difference at all intensities of FVs (t0.2 mV = 2.399, p0.2 mV = 0.032, t0.4 mV = 3.704, p0.4 mV = 0.001, t0.6 mV = 5.779, p0.6 mV < 0.001). Also, the amplitude of FVs was measured over various intensities in order to test whether HG affects presynaptic transmission. In the HG group, there was no deficit of FV responses over the increasing intensities. (Fig. 3d; 1G—10μA: 0.08 ± 0.01, n = 8 slices, 25 μA: 0.11 ± 0.03, n = 8 slices, 50μA: 0.16 ± 0.04, n = 8 slices, 75 μA: 0.28 ± 0.04, n = 8 slices, 100 μA: 0.31 ± 0.05, n = 8 slices, 125 μA: 0.35 ± 0.05, n = 8 slices, 150 μA: 0.40 ± 0.07, n = 7 slices, 175 μA: 0.45 ± 0.07, n = 7 slices, 200 μA: 0.49 ± 0.07, n = 7 slices; HG—10 μA: 0.05 ± 0.02, n = 7 slices, 25 μA: 0.13 ± 0.03, n = 7 slices, 50 μA: 0.20 ± 0.07, n = 7 slices, 75 μA: 0.20 ± 0.06, n = 7 slices, 100 μA: 0.28 ± 0.07, n = 7 slices, 125 μA: 0.29 ± 0.10, n = 7 slices, 150 μA: 0.36 ± 0.11, n = 7 slices, 175 μA: 0.40 ± 0.11, n = 7 slices, 200 μA: 0.42 ± 0.13, n = 7 slices; F1,114 = 1.152, p = 0.285). Our data demonstrate that HG likely damages the postsynaptic function in the SC-CA1 synapse.
Altered LTP in HG
To test whether HG has an effect on synaptic plasticity, we examine short-term and long-term synaptic plasticity in both the 1G and HG groups. When SC was activated by the paired pulse with various intervals, differential paired pulse facilitation (PPF) was observed as the slope of the second fEPSPs (P2) over the first fEPSPs (P1) in both groups (Fig. 4a). There was no statistical significance between the two groups (1G—50 ms: 1.98 ± 0.23, n = 9 slices, 100 ms: 1.77 ± 0.17, n = 9 slices, 250 ms: 1.24 ± 0.07, n = 9 slices, 500 ms: 1.10 ± 0.07, n = 9 slices, HG—50 ms: 1.95 ± 0.20, n = 14 slices, 100 ms: 1.89 ± 0.20, n = 16 slices, 250 ms: 1.22 ± 0.06, n = 13 slices, 500 ms: 1.09 ± 0.06, n = 15 slices; F1,86 = 0.011, p = 0.915). It is remarkable to observe that LTP was diminished in the HG group (Fig. 4b; 1G—baseline: 101.28 ± 2.15, post HFS: 158.71 ± 4.91, n = 11 slices; HG—baseline: 102.27 ± 2.13, post HFS: 110.65 ± 2.87, n = 13 slices; F1,220 = 73.070, p = 0.001). Our results suggest that the gravity shift plays a critical role in the long-term synaptic plasticity.
Discussion
It has been well studied that the vestibular system is greatly affected by altered gravity21,22,56,57,58. The previous study implied that the vestibular organ was the main area influenced by HG in the brain48. Notably, the hippocampus, especially CA1, had electrophysiological and anatomical connections with the vestibular system59,60,61,62,63,64,65,66. For example, lesioned hippocampus aggravated HG-induced motion sickness53. The fact that HG has adverse effects on the brain triggered us to investigate a hippocampal function after the HG conditioning. Our current findings are as follows (1) HG4weeks causes the behavioral deficit in the NOR test; (2) HG4weeks impairs AMPARs/NMDARs-mediated synaptic transmission; (3) HG4weeks group shows abnormal postsynaptic (but normal presynaptic) responses; and (4) HG4weeks alters LTP.
Differential effects of HG on pre- and post-synaptic neurons
Neurotransmitter release is determined by the incidence and pattern of action potentials, depolarization of nerve terminals, and release probability of vesicle machinery67,68,69,70. The previous study investigating the synaptosome in cerebral hemispheres showed that HG alters neurotransmitter release by modulation of neurotransmitter reuptake, indicating a role of HG in a presynaptic mechanism71. However, in our study, there was no significant difference of presynaptic FV amplitude over stimulation intensities and PPF representing presynaptic Ca2+ influx and neurotransmitter release-probability, respectively. It demonstrates that HG does not alter the presynaptic activity at least in CA3–CA1 network. Instead, the strong reliance on postsynaptic AMPAR/NMDAR responses under HG condition depicts a postsynaptic mechanism. This finding is consistent with our early study that HG causes the impaired function of postsynaptic AMPAR and metabotropic glutamate receptors (mGluRs) subtype 1 in the cerebellum72.
A role of HG in cognitive behaviors and plasticity
Previous studies showed that HG causes various physiological changes, such as vestibular function73, signaling pathway in muscles74, and bone formation75 which can lead to altered behaviors. As expected, our research group also observed that HG causes an abnormality of cerebellum-dependent motor coordination72. Now, our view is expanded to investigate a role of HG in cognitive behaviors with a memory test. HG-driven poor performance in NOR may be affected by a defect of memory function because NOR is dominantly dependent on the hippocampus76,77,78,79,80. Our result suggests that HG directly triggers the dysfunction of the hippocampus-dependent cognitive behavior. Given our and other results, HG could accompany multiple, parallel processing of various physiological systems such as HG to vestibular/motor behaviors and HG to cognitive behaviors.
It is previously well known that NMDARs are deeply involved in neural plasticity and often behaviors32,36,81,82,83. A previous study described an increased LTP under short-term exposure of 4G (48 h)44. Meanwhile, we observed that the long-term, but not short-term, exposure of 4G (4 weeks) impairs a cognitive behavior followed by LTP deficit. Prior studies (various gravity levels, 3 weeks) revealed that HG-induced abnormality in various behaviors was sustained even after 15 days from centrifugation45. In our experimental condition, it seems to be worth testing how long the HG effect lasts.
Therapeutic strategy for HG
This study provides the scientific aspect of physiological effects by HG on hippocampus. Long-term gravity shift can cause the impairment of electrophysiological property in the hippocampus and the behavior in the NOR task, and it could be due to a defect of postsynaptic receptors. Therefore, HG-induced impairment may have the potential to be rescued by restoring the function of postsynaptic receptors. We have previously proposed transient potassium channels as a therapeutic target for various brain disorders. It is because the transient potassium channels are electrically counteracting channels to NMDARs and have clinical benefits of minimal interference in a normal synaptic transmission which can be impaired under the pharmacological modulation of NMDARs84,85. A pharmacological approach to enhance an NMDAR function can be further investigated under the condition of altered gravity.
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Acknowledgements
We thank Sungchil Yang for constructive comments on this research. This research was supported by Incheon National University (International Cooperative) Research Grant and the Ministry of Education (no. 2016R1D1A1B04930938) for Sunggu Yang, National Research Foundation of Korea (NRF) funded by the Ministry of Education (No. 2018R1A6A1A03025523) for Kyu-Sung Kim.
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S.Y. and K.-s.K. designed and conceptualized the work. J.L., D.J., and H.J. performed electrophysiological and behavioral experiments and analyzed data. S.Y., K.-s.K., and J.L. wrote and revised the manuscript. All authors reviewed the manuscript.
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Lee, J., Jang, D., Jeong, H. et al. Impairment of synaptic plasticity and novel object recognition in the hypergravity-exposed rats. Sci Rep 10, 15813 (2020). https://doi.org/10.1038/s41598-020-72639-7
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DOI: https://doi.org/10.1038/s41598-020-72639-7
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