Abstract
Early invasive growth along specific anatomical structures, especially the white matter tract, is regarded as one of the main causes of poor therapeutic outcome of people with gliomas. We show that some glioma stem cells (GSCs) are preferentially located along white matter tracts, which exhibit a demyelinated phenotype, at the invasive frontier of glioma tissues. These GSCs are CD133+Notch1+, whereas the nerve fibers express the Notch ligand Jagged1. The Notch-induced transcription factor Sox9 promotes the transcription of SOX2 and the methylation level of the NOTCH1 promoter is attenuated by the upregulation of SOX2 to reinforce NOTCH1 expression in GSCs. This positive-feedback loop in a cohort of glioma subjects is correlated with a poor prognosis. Inhibition of Notch signaling attenuates the white-matter-tract tropism of GSCs. These findings provide evidence indicating that the NOTCH1-SOX2 positive-feedback loop controls GSC invasion along white matter tracts.
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Data Availability
All routine analysis methods are included in Methods. The data that support the findings of this study are presented in the paper and supplementary materials and all raw data are available from the corresponding author upon reasonable request. The five published mRNA expression data sets (Glioblastoma-TCGA-540; Glioma-French-284, GSE16011; Glioblastoma-TCGA-395; Glioblastoma Stemcells-20, GSE15209 and GSE67089) used in this study were obtained from R2, an Affymetrix analysis and visualization platform developed at the Department of Human Genetics at the Academic Medical Center, University of Amsterdam.
Change history
22 March 2019
The original and corrected figures are shown in the accompanying Author Correction.
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Acknowledgements
We thank C.-J. Zhao, S.-M. Yang and X.-D. Liu for their constructive suggestions. This study was supported by grants from the National Key Research and Development Program of China (2016YFA0202104, 2016YFA0101200), the National Natural Science Foundation of China (81570131, 81572880, 61327902), and the Key Clinical Research Program of Southwest Hospital, Army Medical University (Third Military Medical University) of China (SWH2016ZDCX1005, SWH2017ZDCX1003).
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J.W. acquired, analyzed and interpreted data and drafted the manuscript. S.L.X., J.J.D. and L.Y. acquired, analyzed and interpreted data. Y.F.G., Y.S., L.L., Z.Y.Y., X.M.L., J.C., H.L.X. and L.Y. acquired data. Y.Q.Z. performed statistical analysis. H.W., J.N.Z., S.Q.L., Q.K.Y., X.J.Y., T.J. and X.Z. analyzed and interpreted data. X.W.B. analyzed and interpreted data, critically revised the manuscript for intellectual content, obtained funding and supervised the study. S.C.Y. initiated the study concept and design, acquired, analyzed and interpreted data, drafted of the manuscript, critically revised the manuscript for intellectual content, performed statistical analysis, obtained funding, and supervised the study. All authors read and approved the manuscript for publication.
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Supplementary Figure. 1 Spatial relationship between GSCs and nerve fibers.
(a) Schematic diagram of the spatial distribution analysis. (b-c) Spatial relationship between GSCs (PBK+, CDC6+, and CD44+, red). and nerve fibers (NF200+, green) at the invasive frontier of glioma patients as detected by immunofluorescence staining. Three. samples, 3 male, aged from 46 to 57, 3 sections/sample, 10 high-power fields/section. Representative laser confocal microscopy. images of nuclei stained with DAPI (blue) are shown. Scale bar=20 μm (b). Quantitative analysis of the spatial orientation of every GSC. (CDC6, n=445 independent positive cells; PBK, n=426 independent positive cells; CD44, n=450 independent positive cells) to the. nearest nerve fibers (c)
Supplementary Figure 2 Migration, adherence, and invasion of GSCs in vitro and in vivo.
(a) Schematic diagram of the migration assay in vitro. (b) Quantitative analysis of GSC (CD133+, derived from U87, GBM1,and GBM2). migration under high-power field light microscopy showing representative results from 4 independent expriments, the data shown are. the mean±SD. A two-sided Student’s t test was used to generate P values (CS vs. CM. U87-GSC: n=10 independent high-power fields, df=18, t=0.44; GBM1-GSC: n=10 independent high-power fields, df=18, t=0.42; GBM1-GSC: n=10 independent high-power fields, df=18, t=0.79;). CS, culture supernatants; CM, culture media. (c) Adherence of GSCs (CD133+) and non-GSCs (CD133-) to the neurites of neurons cultured in vitro. Representative results from 4 independent expriments, the data shown are the mean±SD. A twosided Student’s t test was used to generate P values (CD133- vs. CD133+, n=25 independent high-power fields, df=48, t=-13). (d, e). The adherence of GSCs (CD133+) to the neurites of neurons cultured in vitro after treatment of Decoy (d) and Notch1-siRNA (e). Representative results from 4 independent exprements, the data shown are the mean±SD. A two-sided Student’s t test was used to. generate P values (PBS vs. Decoy, n=30 independent high-power fields, df=58, t=16; Mock vs. NOTCH1-siRNA, n=30 independent. high-power fields, df=58, t=11). (f) Immunofluorescent staining of CD44 (green) in xenografts of the control group to reveal the invasive. growth of glioma cells (red) along nerve fibers (blue). Two weeks after orthotopic implantation of 1×105 mCherry-labeled GL261 GSCs. into the right striatum adjacent to the corpus callosum of Thy1-EGFP transgenic mice. Five mice per group, 3 sections per mouse, 3-6. high-power fields per section, n=54 high-power fields. Representative laser confocal microscopy images of nuclei stained with DAPI. (gray) are shown. Scale bar=20 μm. (G) 3D diagram of the orthotopic xenograft. View of the horizontal plane and view angle with an. 80º horizontal plane. 3D diagram of mouse brain derived from Brain explore 2 (Allen Institute for Brain Science). The red circle denotes. the site of implantation
Supplementary Figure. 3 Relationship between SOX2 and Notch receptors (NOTCH1–4) and transcription factors (SOX9, HEY1, HEY2, and HES1) expression.
The data were derived from 3 Affymetrix DNA microarray datasets (Tumor Glioblastoma - TCGA - 540, n= 540 biologically independent. patients; Tumor Glioma - French - 284, n= 284 biologically independent patients; and Tumor Glioblastoma - TCGA – 395, n= 395. biologically independent patients). Pearson correlation analysis (2-tailed) was used to generate P values and correlation coefficients (r)
Supplementary Figure 4 The mechanism underlying NOTCH1 upregulation of the transcription of SOX2.
(a) Luciferase reporter assay of the 5’-promoter of SOX2. NOTCH1OE, GBM2 non-GSC cells stably overexpressing NOTCH1 via. pcDNA 3.1-NOTCH1; Vector, GBM2 non-GSC cells stably transfected with pcDNA3.1 empty vector; n=3 biologically independent. samples, the data are the mean±SD. (b) The similarity of the Sox2 promoter region between mouse and human. (c) The putative. binding sites of RBP-Jκ located at the promoter of the human SOX2 gene. (d) Chromatin. immunoprecipitation (ChIP) analysis in GSCs. derived from GBM2 cells. PCR primers were designed to surround predicted RBP-Jκ-binding sites 1 and 2 on the SOX2 promoter. Nonspecific IgG and anti-RNA polymerase II were used as controls. Three times these experiments were repeated independently with similar results. m, DNA ladder
Supplementary Figure 5 Immunofluorescent staining of Notch1, Sox9, and Sox2 in GSCs derived from GBM 1-3.
Representative lase confocal microscopy images of nuclei stained with hoechst33342 are shown. Scale bar=20 μm. (A) Double. immunofluorescent staining of Notch1 (red), Sox9 (red or green), and Sox2 (red or green) in GSCs derived from GBM 2 and GBM 3 cell. lines. Right panel: expanded view of the inset regions (white box) shown in the left panel. Inset1-3, scale bar=10 μm; Inset4-6, scale. bar=2 μm._Three times these experiments were repeated independently with similar results. (B) Triple immunofluorescent staining of. Notch1 (green), Sox9 (blue), and Sox2 (red) in GSCs derived from GBM 1 and GBM3. Scale bar=20 μm. Three times these. experiments were repeated independently with similar results
Supplementary Figure 6
Schematic diagram of orthotopic xenografts
Supplementary Figure 7 Luciferase reporter assays of the enhancers of NOTCH1.
(A) The putative enhancer binding sites of Sox2 located in the introns and exons of the NOTCH1 gene. (B) Luciferase activity of GBM2. non-GSC cells after different fragments containing the putative enhancers were cloned into a minimal promoter of the pGL-4 luciferase. vector and then co-transfected with pcDNA 3.1-SOX2, n=3 biologically independent samples. Center line represents the median, lower. and upper limits of the box represent the 25th and 75th percentiles, and whiskers show maximum and minimum
Supplementary Figure 8 Schematic diagram of the reciprocal activation between SOX2 and Notch signaling determining the WM tract tropism of GSCs.
CD133 and Notch1 double-positive GSCs were preferentially located along the Notch ligand Jagged1 expressed WM tracts, which. exhibited a demyelinated phenotype, at the invasive frontier of glioma tissues. The Notch-induced transcription factor Sox9 promoted. the transcription of SOX2 and the methylation level of the NOTCH1 promoter was attenuated by the upregulation of SOX2 to reinforce. NOTCH1 expression in GSCs
Supplementary Figure 9 Distribution of Sox9+ cells in different regions of human glioma samples.
Seven samples, 3 sections per sample, 1-20 high-power fields per section. Upper panel: Representative image of IHC staining. Dottedline. boxes (1-3) indicate the center, invasive frontier, and distant site of the tumor mass. Scale bar=200 μm. Lower panel: expanded. view of the inset regions (red box) shown in the upper panel. Scale bar=50 μm
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Wang, J., Xu, SL., Duan, JJ. et al. Invasion of white matter tracts by glioma stem cells is regulated by a NOTCH1–SOX2 positive-feedback loop. Nat Neurosci 22, 91–105 (2019). https://doi.org/10.1038/s41593-018-0285-z
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DOI: https://doi.org/10.1038/s41593-018-0285-z
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