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Polarly localized kinase SGN1 is required for Casparian strip integrity and positioning

Nature Plants volume 2, Article number: 16113 (2016) Cite this article

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Abstract

Casparian strips are precisely localized and aligned ring-like cell wall modifications in the root of all higher plants. They set up an extracellular diffusion barrier analogous to animal tight junctions, and are crucial for maintaining the homeostatic capacity of plant roots. Casparian strips become localized because of the formation of a highly stable plasma membrane domain, consisting of a family of small transmembrane proteins called Casparian strip membrane domain proteins (CASPs). Here we report a large-scale forward genetic screen directly visualizing endodermal barrier function, which allowed us to identify factors required for the formation and integrity of Casparian strips. We present the identification and characterization of one of the mutants, schengen1 (sgn1), a receptor-like cytoplasmic kinase that we show localizes in a strictly polar fashion to the outer plasma membrane of endodermal cells and is required for the positioning and correct formation of the centrally located CASP domain.

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Figure 1: Isolation of Casparian-strip-impaired mutants and molecular features of SGN1.
Figure 2: SGN1 is a kinase required for CASP domain integrity and positioning.
Figure 3: SGN1 specifically expresses in differentiating endodermal cells and localizes to the cortex-facing plasma membrane.
Figure 4: Membrane localization and kinase activity are essential for SGN1 function.
Figure 5: Schematic of possible SGN1 roles in CASP domain formation and positioning.

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Acknowledgements

We thank the Central Imaging Facility (CIF) and the Genome Technology Facility (GTF) of the University of Lausanne for expert technical support, as well as V. Dénervaud Tendon and S. Ammar for technical assistance. We wish to thank D. Roppolo, E. N. M. Dohmann, Y. Lee and D. Salt for helpful discussions; D. Salt for sharing material before publication; and T. Kohchi and R. Nishihama from Kyoto University for unpublished sequences. This work was funded by grants from the Swiss National Science Foundation (SNSF) and the European Research Council (ERC) grants Plant-Memb-Traff and ENDOFUN to N.G. J.E.M.V. was supported by a Marie-Curie intra-European fellowship, M.B. by a EMBO long-term postdoctoral fellowship, S.F. by a JSPS postdoctoral fellowship for research abroad and V.G.D. by the Fundación Alfonso Martín Escudero.

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Author notes

  1. Julien Alassimone, Maritza van Dop, Joop E. M. Vermeer & Luca Santuari

    Present address: †Present addresses: Department of Biology, Stanford University, Stanford, California, 94305, USA (J.A.); Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703HA Wageningen, The Netherlands (M.v.D.); Department of Plant and Microbial Biology, University of Zürich, Zollikerstrasse 107, 8008 Zürich, Switzerland (J.E.M.V.); Plant Developmental Biology, Wageningen University Research, Building 107 (Radix), Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands (L.S.),

  2. Julien Alassimone and Satoshi Fujita: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Plant Molecular Biology, Biophore, Campus UNIL-Sorge, University of Lausanne, CH-1015 Lausanne, Switzerland

    Julien Alassimone, Satoshi Fujita, Verónica G. Doblas, Maritza van Dop, Marie Barberon, Lothar Kalmbach, Joop E. M. Vermeer, Nelson Rojas-Murcia, Luca Santuari, Christian S. Hardtke & Niko Geldner

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Contributions

N.G. and J.A. initiated the project. J.A., S.F., V.G.D., J.E.M.V. and N.G. designed experiments. J.A., S.F., V.G.D, M.v.D., M.B., L.K., J.E.M.V., N.R.M, L.S., C.H. and N.G. performed experiments and interpreted the results. L.K. provided unpublished plant materials. Manuscript written by J.A., S.F. and N.G. All authors edited and commented on the manuscript.

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Correspondence to Niko Geldner.

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Supplementary Figs 1–4, Supplementary Table 1 and Supplementary Materials and Methods. (PDF 1390 kb)

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Alassimone, J., Fujita, S., Doblas, V. et al. Polarly localized kinase SGN1 is required for Casparian strip integrity and positioning. Nature Plants 2, 16113 (2016). https://doi.org/10.1038/nplants.2016.113

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