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Benchmarking a luciferase complementation assay for detecting protein complexes

Nature Methods volume 8, pages 990–992 (2011)Cite this article

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To the Editor:

Mapping protein-protein interactions (PPIs) has proven instrumental in functional proteomics. Large PPI databases have been established by literature mining or using high-throughput screening methods; this information requires cross-validation to increase coverage and accuracy. Previously published papers in Nature Methods have argued for the use of defined reference sets for the benchmarking of screening methods for PPIs1,2.

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Figure 1: Benchmarking a luciferase complementation assay against reference sets.
Figure 2: Detection of ternary protein complexes.

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Acknowledgements

We thank M. Vidal, P. Braun, D. Hill and members of Dana-Farber Cancer Institute Center for Cancer Systems Biology for providing the PRS entry clones. This work was supported in part by funding from the Institut Pasteur, and by grants from the Ligue Nationale Contre le Cancer (grants R05/75-129 and RS07/75-75), the Association pour la Recherche sur le Cancer (grants ARC A09/1/5031 and 4867), and the Agence Nationale de la Recherche (EPI-HPV-3D and NAB-HPV programs). G.N. was a recipient of the Conseil Général de la Vienne and Pasteur Weizmann foundation. C.R. was a recipient of a Ministère de l'Education Nationale de la Recherche et de la Technologie fellowship.

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  1. Patricia Cassonnet and Caroline Rolloy: These authors contributed equally to this work.

Authors and Affiliations

  1. Unité de Génétique, Papillomavirus et Cancer Humain, Institut Pasteur, Paris, France

    Patricia Cassonnet, Caroline Rolloy, Gregory Neveu, Mandy Muller, Caroline Demeret, Guillaume Gaud, Françoise Vuillier, Michel Favre & Yves Jacob

  2. Unité de Génomique Virale et Vaccination, Institut Pasteur, Centre National de la Recherche Scientifique, Unité de Recherche Associée 3015, Paris, France

    Pierre-Olivier Vidalain & Fréderic Tangy

  3. Infection Mapping Project Team, Université Lyon 1, Institut Fédératif de Recherche 128, Institut National de la Santé et de la Recherche Médicale, Unité 851, Lyon, France

    Thibault Chantier, Johann Pellet & Vincent Lotteau

  4. Groupe Projets et Développements en Bioinformatique, Institut Pasteur, Paris, France

    Louis Jones

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  1. Patricia Cassonnet
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Correspondence to Yves Jacob.

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Supplementary Figures 1–4, Supplementary Tables 1–7, Supplementary Methods (PDF 3544 kb)

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Cassonnet, P., Rolloy, C., Neveu, G. et al. Benchmarking a luciferase complementation assay for detecting protein complexes. Nat Methods 8, 990–992 (2011). https://doi.org/10.1038/nmeth.1773

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