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Reply to “Properties of a disease-specific prion probe”

Nature Medicine volume 10, pages 11–12 (2004)Cite this article

Paramithiotis et al. reply:

We did indeed use 'regular' PrP-specific antibodies for the immunoblotting and ELISA detection of PrPSc captured by our YYR-specific antibodies. However, we were also able to detect native PrPSc at the surface of scrapie-infected sheep dendritic cells using direct immunostaining protocols1. In addition, because YYR-specific antibodies do not react with brain homogenate by immunoblot, and do not seem to possess significant surface reactivity on normal dissociated neurons or lymphoid cells, this epitope is apparently rare in the structural proteome.

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References

  1. Paramithiotis, E. et al. Nat. Med. 9, 893–899 (2003).

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  2. Korth, C. et al. Nature 390, 74–77 (1997).

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Authors and Affiliations

  1. Caprion Pharmaceuticals, 7150 Alexander-Fleming, St-Laurent, Quebec, H4S 2C8, Canada

    Eustache Paramithiotis, Marc Pinard, Sylvie LaBoissiere, Julie Lamontagne, Leslie H Kondejewski, Maria Papadopoulos, Ashkan Haghighat, Harry C Ledebur & Neil R Cashman

  2. IDEXX Laboratories, 1 IDEXX Drive, Westbrook, 04092, Maine, USA

    Trebor Lawton, Valerie L Leathers, Lisa A Estey, Gregory P Francoeur, Stephen J Spatz & Quentin Tonelli

  3. Centre for Research in Neurodegenerative Diseases, 6 Queen's Park Crescent West, University of Toronto, Toronto, M5S 3H2, Ontario, Canada

    Wen-Quan Zou, Marty T Lehto & Neil R Cashman

  4. The National Creutzfeldt-Jakob Disease Surveillance Unit, Western General Hospital, Crewe Road, Edinburgh, EH4 2XU, UK

    Mark Head, Robert Will & James Ironside

  5. USDA-ARS-ADRU, 3003 ADBF, Washington State University, Pullman, 99164-6630, Washington, USA

    Katherine O'Rourke

  6. Department of Medical Biophysics, University of Toronto, Ontario Cancer Institute, 610 University Avenue, Toronto, M5G 2M9, Ontario, Canada

    Avi Chakrabartty & Neil R Cashman

  7. Sunnybrook & Women's College Health Sciences Centre, University of Toronto, 2075 Bayview Avenue, Toronto, M4N 3M5, Ontario, Canada

    Neil R Cashman

Authors
  1. Eustache Paramithiotis
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  2. Marc Pinard
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Competing interests

This response letter represents an ongoing University-Industry collaboration between scientists at the University of Toronto and two biotechnology companies (Caprion Pharmaceuticals of Montreal, Canada, and Idexx Laboratories of Portland, Maine). N.R.C., the senior author, is a professor at the University of Toronto, as well as 'Founder and Scientific Advisor' of Caprion Pharmaceuticals, from which he has derived partial salary and laboratory operating grants. The commercial aspects of this discovery are being developed under license from Caprion to Idexx and Ortho Clinical Diagnostics.

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Paramithiotis, E., Pinard, M., Lawton, T. et al. Reply to “Properties of a disease-specific prion probe”. Nat Med 10, 11–12 (2004). https://doi.org/10.1038/nm0104-11b

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  • DOI: https://doi.org/10.1038/nm0104-11b

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