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Effect of Rho on Transcription of Bacterial Operons

Nature New Biology volume 241, pages 260–264 (1973)Cite this article

Abstract

In vitro experiments suggest that termination of transcription by the E. coli rho factor probably accounts for the natural polarity observed in certain bacterial operons, arrest of transcription at the end of operons and the severe polar properties of some insertion mutations.

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References

  1. Roberts, J., Cold Spring Harbor Symp. Quant. Biol., 35, 121 (1970).

    Article  CAS  Google Scholar 

  2. Takanami, M., Okamoto, T., and Sugiura, M., J. Mol. Biol., 62, 81 (1972).

    Article  Google Scholar 

  3. Roberts, J., Nature, 224, 1168 (1969).

    Article  CAS  Google Scholar 

  4. Buttin, G., J. Mol. Biol., 7, 183 (1963).

    Article  CAS  Google Scholar 

  5. Echols, H., Reznichek, J., and Adhya, S., Proc. US Nat. Acad. Sci., 50, 286 (1963).

    Article  CAS  Google Scholar 

  6. Wilson, D. B., and Hogness, D. S., J. Biol. Chem., 244, 2132 (1969).

    CAS  PubMed  Google Scholar 

  7. Saito, S., Ozutsumi, M., and Kurahashi, K., J. Biol. Chem., 242, 2362 (1967).

    CAS  PubMed  Google Scholar 

  8. Wilson, D. B., and Hogness, D. S., J. Biol. Chem., 244, 2137 (1967).

    Google Scholar 

  9. Shapiro, J. A., and Adhya, S. L., Genetics, 62, 249 (1969).

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Michaelis, G., and Starlinger, P., Mol. Gen. Genet., 100, 210 (1967).

    Article  CAS  Google Scholar 

  11. Miller, Z., Varmus, H. L., Parks, J., Perlman, R. L., and Pastan, I., J. Biol. Chem., 246, 2898 (1971).

    CAS  PubMed  Google Scholar 

  12. Nissley, P., Anderson, W. B., Gottesman, M. E., Perlman, R. L., and Pastan, I., J. Biol. Chem., 246, 4671 (1972).

    Google Scholar 

  13. Nakanishi, S., Adhya, S. L., Gottesman, M. E., and Pastan, I., Proc. US Nat. Acad. Sci. (in the press).

  14. Jacob, F., and Monod, J., J. Mol. Biol., 3, 318 (1961).

    Article  CAS  Google Scholar 

  15. Ullmann, A., Goldberg, M. E., Perrin, D., and Monod, J., Biochemistry, 1, 261 (1968).

    Article  Google Scholar 

  16. Zabin, I., and Fowler, A. V., in The Lactose Operon (edit. by Beckwith, J. R., and Zipser, D.), 27 (Cold Spring Harbor Laboratory, New York, 1970).

    Google Scholar 

  17. Jones, T. D. H., and Kennedy, E. P., J. Biol. Chem., 244, 5981 (1969).

    CAS  PubMed  Google Scholar 

  18. Brown, J. L., Brown, D. M., and Zabin, I., J. Biol. Chem., 242, 4524 (1967).

    Google Scholar 

  19. De Crombrugghe, B., Chen, B., Anderson, W. B., Nissley, P., Gottesman, M., Pastan, I., and Perlman, R., Nature New Biology, 231, 139 (1971).

    Article  CAS  Google Scholar 

  20. Adhya, S. L., and Shapiro, J. A., Genetics, 62, 231 (1969).

    CAS  PubMed  PubMed Central  Google Scholar 

  21. Shapiro, J. A., J. Mol. Biol., 40, 93 (1969).

    Article  CAS  Google Scholar 

  22. Jordan, E., Saedler, H., and Starlinger, P., Mol. Gen. Genet., 102, 353 (1968).

    Article  CAS  Google Scholar 

  23. Malamy, M. H., in The Lactose Operon (edit. by Beckwith, J. R., and Zipser, D.), 359 (Cold Spring Harbor Laboratory, New York, 1970).

    Google Scholar 

  24. Brachet, P., Eisen, H., and Rambach, A., Mol. Gen. Genet., 108, 266 (1970).

    Article  CAS  Google Scholar 

  25. Jordan, E., Saedler, H., and Starlinger, P., Mol. Gen. Genet., 100, 296 (1967).

    Article  CAS  Google Scholar 

  26. Ideda, H., Nature New Biology, 234, 198 (1971).

    Article  Google Scholar 

  27. Travers, A. A., Kamen, R. I., and Shleif, R. F., Nature, 228, 748 (1972).

    Article  Google Scholar 

  28. Haseltine, W. A., Nature, 235, 329 (1972).

    Article  CAS  Google Scholar 

  29. Dunn, J. J., McAllister, W. T., and Bautz, E. K. F., Virology, 48, 112 (1972).

    Article  CAS  Google Scholar 

  30. Travers, A. A., Nature, 225, 1009 (1970).

    Article  CAS  Google Scholar 

  31. Richardson, J. P., Cold Spring Harbor Symp. Quant. Biol., 35, 127 (1970).

    Article  CAS  Google Scholar 

  32. Goff, C. G., and Minkley, E. G., in RNA Polymerase and Transcription, LePetit Colloqu. Biol. Med. (edit. by Silvestri, L.), 124 (North-Holland, Amsterdam and London, 1970).

    Google Scholar 

  33. Okamoto, T., Sugiura, M., and Takanami, M., Biochemistry, 9, 3533 (1970).

    Article  CAS  Google Scholar 

  34. Goldberg, A. R., and Hurwitz, J., J. Biol. Chem., 247, 5637 (1972).

    CAS  PubMed  Google Scholar 

  35. Nishi, A., and Zabin, I., Biochem. Biophys. Res. Commun., 13, 320 (1963).

    Article  CAS  Google Scholar 

  36. Varmus, H. E., Perlman, R. L., and Pastan, I. H., J. Biol. Chem., 245, 6366 (1970).

    CAS  PubMed  Google Scholar 

  37. Hill, C. W., and Echols, H., J. Mol. Biol., 19, 38 (1966).

    Article  CAS  Google Scholar 

  38. Erickson, R. P., Biochem. J., 120, 255 (1970).

    Article  CAS  Google Scholar 

  39. Zipser, D., The Lactose Operon (edit. by Beckwith, J. R., and Zipser, D.), 221 (Cold Spring Harbor Laboratory, New York, 1970).

    Google Scholar 

  40. Wetekam, W., Staack, K., and Ehring, R., Mol. Gen. Genet., 116, 258 (1972).

    Article  CAS  Google Scholar 

  41. De Crombrugghe, B., Varmus, H. E., Perlman, R. L., and Pastan, I., Biochem. Biophys. Res. Commun., 38, 694 (1970).

    Article  Google Scholar 

  42. Berg, D., Barrett, K., and Chamberlin, M., Methods in Enzymology, 21, 506 (1971).

    Article  Google Scholar 

  43. Anderson, W. B., Schneider, A. B., Emmer, M., Perlman, R. L., and Pastan, I., J. Biol. Chem., 246, 5929 (1971).

    CAS  Google Scholar 

  44. Bovre, K., and Szybalski, W., Methods in Enzymology, 21, 350 (1971).

    Article  Google Scholar 

Download references

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Authors and Affiliations

  1. Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland, 20014

    BENOIT DE CROMBRUGGHE, SANKAR ADHYA, MAX GOTTESMAN & IRA PASTAN

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  1. BENOIT DE CROMBRUGGHE
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  2. SANKAR ADHYA
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  3. MAX GOTTESMAN
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  4. IRA PASTAN
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DE CROMBRUGGHE, B., ADHYA, S., GOTTESMAN, M. et al. Effect of Rho on Transcription of Bacterial Operons. Nature New Biology 241, 260–264 (1973). https://doi.org/10.1038/newbio241260a0

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  • DOI: https://doi.org/10.1038/newbio241260a0

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