Abstract
A cell free system has been developed in which only the components of the lactose operon are required for its transcription. Results obtained with this system suggest that the DNA of the operon contains a new regulator site, at which the cyclic AMP control system acts.
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References
de Crombrugghe, B., Chen, B., Gottesman, M., Pastan, I., Varmus, H. E., Emmer, M., and Perlman, R. L., Nature, 230, 37 (1971).
Shapiro, J., Machattie, L., Eron, L., Ihler, G., Ippen, K., and Beckwith, J., Nature, 224, 768 (1969).
Emmer, M., de Crombrugghe, B., Pastan, I., and Perlman, R. L., Proc. US Nat. Acad. Sci., 66, 480 (1970).
Zubay, G., Schwartz, D., and Beckwith, J., Proc. US Nat. Acad. Sci., 66, 104 (1970).
Ohshima, Y., Horiuchi, T., Iida, Y., and Kameyama, T., Mol. Gen. Genetics, 106, 307 (1970).
Arditti, R. R., Eron, L., Zubay, G., Tocchini-Valentini, G., Connaway, S., and Beckwith, J. R., Cold Spring Harbor Symp. Quant. Biol., 35, 437 (1970).
Eron, L., Arditti, R. R., Zubay, G., Connaway, S., and Beckwith, J. A., Proc. US Nat. Acad. Sci., 68, 215 (1971).
Echols, H., Pilarski, L., and Cheng, P. Y., Proc. US Nat. Acad. Sci., 59, 1891 (1968).
Ptashne, M., Nature, 214, 232 (1967).
Gilbert, W., and Muller-Hill, B., Proc. US Nat. Acad. Sci., 58, 2415 (1967).
Riggs, A. D., Bourgeois, S., Newby, R. F., and Cohn, M., J. Mol. Biol., 34, 365 (1968).
Chadwick, P., Pirotta, V., Hopkins, I., Steinberg, R., and Ptashne, M., Cold Spring Harbor Symp. Quant. Biol., 35, 283 (1970).
Riggs, A. D., Newby, R. F., and Bourgeois, S., J. Mol. Biol., 51, 303 (1970).
Burgess, R. B., J. Biol. Chem., 244, 6160 (1969).
Perlman, R. L., de Crombrugghe, B., and Pastan, I., Nature, 223, 810 (1969).
Silverstone, A. E., Magasanik, B., Reznikoff, W. S., Miller, J. H., and Beckwith, J. R., Nature, 221, 1012 (1969).
Silverstone, A. E., Arditti, R., and Magasanik, B., Proc. US Nat. Acad. Sci., 66, 773 (1970).
Jacquet, M., and Kepes, A., Biochem. Biophys. Res. Commun., 36, 84 (1969).
Bautz, E. K. F., and Bautz, F. A., Nature, 226, 1219 (1970).
Travers, A. A., Kamen, R. I., and Schleif, R. F., Nature, 228, 748 (1970).
Parks, J., Gottesman, M., Perlman, R. L., and Pastan, I., J. Biol. Chem. (in the press).
Pastan, I., de Crombrugghe, B., Chen, B., Anderson, W., Parks, I. J., Nissley, P., Straub, M., Gottesman, M., and Perlman, R. L., Proc. Third Ann. Biochem.-PCRI Winter Symp. (University I of Miami Press, 1971).
Martelo, O. J., Woo, S. C. L., Reimann, E. M., and Davie, E. W., Biochemistry, 9, 4807 (1970).
Jacob, F., and Monod, J., J. Mol. Biol., 3, 318 (1961).
Varmus, H. E., Perlman, R. L., and Pastan, L., J. Biol. Chem., 245, 2259 (1970).
de Crombrugghe, B., Varmus, H. E., Perlman, R. L., and Pastan, I., Biochem. Biophys. Res. Commun., 38, 894 (1970).
Gillespie, D., and Spiegelman, S., J. Mol. Biol., 12, 829 (1965).
Hradecna, Z., and Szybalski, W., Virology, 32, 663 (1967).
Roberts, J. W., Nature, 224, 1168 (1969).
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de CROMBRUGGHE, B., CHEN, B., ANDERSON, W. et al. Lac DNA, RNA Polymerase and Cyclic AMP Receptor Protein, Cyclic AMP, Lac Repressor and Inducer are the Essential Elements for Controlled Lac Transcription. Nature New Biology 231, 139–142 (1971). https://doi.org/10.1038/newbio231139a0
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DOI: https://doi.org/10.1038/newbio231139a0
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