Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Lac DNA, RNA Polymerase and Cyclic AMP Receptor Protein, Cyclic AMP, Lac Repressor and Inducer are the Essential Elements for Controlled Lac Transcription

Abstract

A cell free system has been developed in which only the components of the lactose operon are required for its transcription. Results obtained with this system suggest that the DNA of the operon contains a new regulator site, at which the cyclic AMP control system acts.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. de Crombrugghe, B., Chen, B., Gottesman, M., Pastan, I., Varmus, H. E., Emmer, M., and Perlman, R. L., Nature, 230, 37 (1971).

    CAS  Google Scholar 

  2. Shapiro, J., Machattie, L., Eron, L., Ihler, G., Ippen, K., and Beckwith, J., Nature, 224, 768 (1969).

    Article  CAS  Google Scholar 

  3. Emmer, M., de Crombrugghe, B., Pastan, I., and Perlman, R. L., Proc. US Nat. Acad. Sci., 66, 480 (1970).

    Article  CAS  Google Scholar 

  4. Zubay, G., Schwartz, D., and Beckwith, J., Proc. US Nat. Acad. Sci., 66, 104 (1970).

    Article  CAS  Google Scholar 

  5. Ohshima, Y., Horiuchi, T., Iida, Y., and Kameyama, T., Mol. Gen. Genetics, 106, 307 (1970).

    Article  CAS  Google Scholar 

  6. Arditti, R. R., Eron, L., Zubay, G., Tocchini-Valentini, G., Connaway, S., and Beckwith, J. R., Cold Spring Harbor Symp. Quant. Biol., 35, 437 (1970).

    Article  CAS  Google Scholar 

  7. Eron, L., Arditti, R. R., Zubay, G., Connaway, S., and Beckwith, J. A., Proc. US Nat. Acad. Sci., 68, 215 (1971).

    Article  CAS  Google Scholar 

  8. Echols, H., Pilarski, L., and Cheng, P. Y., Proc. US Nat. Acad. Sci., 59, 1891 (1968).

    Article  Google Scholar 

  9. Ptashne, M., Nature, 214, 232 (1967).

    Article  CAS  Google Scholar 

  10. Gilbert, W., and Muller-Hill, B., Proc. US Nat. Acad. Sci., 58, 2415 (1967).

    Article  CAS  Google Scholar 

  11. Riggs, A. D., Bourgeois, S., Newby, R. F., and Cohn, M., J. Mol. Biol., 34, 365 (1968).

    Article  CAS  Google Scholar 

  12. Chadwick, P., Pirotta, V., Hopkins, I., Steinberg, R., and Ptashne, M., Cold Spring Harbor Symp. Quant. Biol., 35, 283 (1970).

    Article  CAS  Google Scholar 

  13. Riggs, A. D., Newby, R. F., and Bourgeois, S., J. Mol. Biol., 51, 303 (1970).

    Article  CAS  Google Scholar 

  14. Burgess, R. B., J. Biol. Chem., 244, 6160 (1969).

    CAS  PubMed  Google Scholar 

  15. Perlman, R. L., de Crombrugghe, B., and Pastan, I., Nature, 223, 810 (1969).

    Article  CAS  Google Scholar 

  16. Silverstone, A. E., Magasanik, B., Reznikoff, W. S., Miller, J. H., and Beckwith, J. R., Nature, 221, 1012 (1969).

    Article  CAS  Google Scholar 

  17. Silverstone, A. E., Arditti, R., and Magasanik, B., Proc. US Nat. Acad. Sci., 66, 773 (1970).

    Article  CAS  Google Scholar 

  18. Jacquet, M., and Kepes, A., Biochem. Biophys. Res. Commun., 36, 84 (1969).

    Article  CAS  Google Scholar 

  19. Bautz, E. K. F., and Bautz, F. A., Nature, 226, 1219 (1970).

    Article  CAS  Google Scholar 

  20. Travers, A. A., Kamen, R. I., and Schleif, R. F., Nature, 228, 748 (1970).

    Article  CAS  Google Scholar 

  21. Parks, J., Gottesman, M., Perlman, R. L., and Pastan, I., J. Biol. Chem. (in the press).

  22. Pastan, I., de Crombrugghe, B., Chen, B., Anderson, W., Parks, I. J., Nissley, P., Straub, M., Gottesman, M., and Perlman, R. L., Proc. Third Ann. Biochem.-PCRI Winter Symp. (University I of Miami Press, 1971).

    Google Scholar 

  23. Martelo, O. J., Woo, S. C. L., Reimann, E. M., and Davie, E. W., Biochemistry, 9, 4807 (1970).

    Article  CAS  Google Scholar 

  24. Jacob, F., and Monod, J., J. Mol. Biol., 3, 318 (1961).

    Article  CAS  Google Scholar 

  25. Varmus, H. E., Perlman, R. L., and Pastan, L., J. Biol. Chem., 245, 2259 (1970).

    CAS  Google Scholar 

  26. de Crombrugghe, B., Varmus, H. E., Perlman, R. L., and Pastan, I., Biochem. Biophys. Res. Commun., 38, 894 (1970).

    Article  CAS  Google Scholar 

  27. Gillespie, D., and Spiegelman, S., J. Mol. Biol., 12, 829 (1965).

    Article  CAS  Google Scholar 

  28. Hradecna, Z., and Szybalski, W., Virology, 32, 663 (1967).

    Article  Google Scholar 

  29. Roberts, J. W., Nature, 224, 1168 (1969).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

de CROMBRUGGHE, B., CHEN, B., ANDERSON, W. et al. Lac DNA, RNA Polymerase and Cyclic AMP Receptor Protein, Cyclic AMP, Lac Repressor and Inducer are the Essential Elements for Controlled Lac Transcription. Nature New Biology 231, 139–142 (1971). https://doi.org/10.1038/newbio231139a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/newbio231139a0

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing