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A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells

Nature Biotechnology volume 14, pages 1133–1138 (1996)Cite this article

Abstract

An optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (Beta vulgaris L.). A polyethylene glycol-mediated DNA transformation technique could be applied to protoplast populations enriched specifically for a single totipotent cell type derived from stomatal guard cells, to achieve high transformation frequencies. Bialaphos resistance, conferred by the pat gene, produced a highly efficient selection system. The majority of plants were obtained within 8 to 9 weeks and were appropriate for plant breeding purposes. All were resistant to glufosinate-ammonium–based herbicides. Detailed genomic characterization has verified transgene integration, and progeny analysis showed Mendelian inheritance.

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Author notes

  1. Robert D. Hall: r.d.hall@cpro.dlo.nl

Authors and Affiliations

  1. Department of Cell Biology, DLO-Centre for Plant Breeding and Reproduction Research (CPRO-DLO), P.O. Box 16, 6700 AA, Wageningen, The Netherlands

    Tjitske Riksen-Bruinsma, Arjen van Tunen & Frans A. Krens

  2. SES Europe NV/SA, Industriepark 15, B-3300, Tienen, Belgium

    Guy J. Weyens, Inge J. Rosquin, Pascale N. Denys, Jean E. Lathouwers & Marc P. Lefèbvre

  3. Zeneca Seeds, Jealott's Hill Research Station, Bracknell, Berkshire, RG12 6EY, UK

    Ian J. Evans & Jim M. Dun well

  4. Department of Agricultural Botany, University of Reading, Whiteknights, P.O. Box 221, Reading, RG6 6AS, UK

    Jim M. Dun well

Authors
  1. Robert D. Hall
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  2. Tjitske Riksen-Bruinsma
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  3. Guy J. Weyens
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  5. Pascale N. Denys
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  6. Ian J. Evans
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  7. Jean E. Lathouwers
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  8. Marc P. Lefèbvre
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  9. Jim M. Dun well
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  10. Arjen van Tunen
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  11. Frans A. Krens
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Hall, R., Riksen-Bruinsma, T., Weyens, G. et al. A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells. Nat Biotechnol 14, 1133–1138 (1996). https://doi.org/10.1038/nbt0996-1133

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